GC MS/MS analysis of Bioactive Compounds in Alcoholic Seed Extract of Gauzuma ulmifolia Lam

Abstract

Introduction:Gauzuma ulmifolia Lam. (GU) belonging to the family Malvaceae, commonly called Bastard Cedar is native to tropical American countries. It was introduced into India more than 100 years ago. Methods: In the present study, alcoholic seed extract of Gauzuma ulmifolia Lam. was subjected to GC MS/MS analysis and chemical compounds were characterized. Results: Totally sixteen compounds were characterized. Chemical analysis of the extract showed that it includes bioactive compounds like D-Asarinin (65.02 %), 2,6-Bis (3,4-methylenedioxyphenyl)-3,7-dioxabicyclo (3.3.0) octane (20.12 %), 1-Dodecanone, 2-(imidazol-1-yl)-1-(4-methoxyphenyl) (7.54 %) and o-Anisic acid, tridec-2-ynyl ester (3.33 %) as major constituents. Minor components such as Vitamin E, γ -Tocopherol, Ergost-5-en-3-ol, (3β), Hexadecanoic acid, methyl ester is also present. Conclusion: Based on the above results, the seeds of this plant could posse's oxygen (92.27 %), hydrocarbon (0.19 %) and nitrogen (7.54 %) derivatives of volatile principle. This is the first-time report on the composition of seed of GU.

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“…9 GC-MS Analysis: The GC-MS instrument consists of two main components. The gas chromatography portion separates different compounds in the sample into pulses of pure chemicals based on their volatility by flowing an inert gas (mobile phase), which carries the sample, through a stationary phase fixed in column 10 . Spectra of compounds are collected as they exit a chromatographic column by the mass spectrometer, which identifies and quantifies the chemicals according to their mass-tocharge ratio (m/z).…”