GC/MS identification and quantification of constituents of bacterial lipids and glycoconjugates obtained after methanolysis as heptafluorobutyrate derivatives

Bohin, Anne; Bouchart, Franck; Richet, Colette; Kol, Osarath; Leroy, Y.; Timmerman, Philippe; Huet, Guillemette; Bohin, Jean-Pierre; Zanetta, Jean‐Pierre

doi:10.1016/j.ab.2005.02.017

Cited by 26 publications

(11 citation statements)

References 30 publications

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“…Mass analysis was performed at 70 eV with an ion source temperature of 200 • C. Integration of the peaks was performed on the total ion chromatogram using Xcalibur software (Thermo Finnigan). The results had a procedure error of <5%, in agreement with similar reports [16].…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms)supporting

confidence: 92%

Influence of selected antimicrobials on the viability, endotoxicity and lipopolysaccharide composition of Pseudomonas aeruginosa in vitro

Abraham

Venter

Lues

et al. 2009

International Journal of Antimicrobial Agents

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Section: Gas Chromatography-mass Spectrometry (Gc-ms)supporting

confidence: 92%

Influence of selected antimicrobials on the viability, endotoxicity and lipopolysaccharide composition of Pseudomonas aeruginosa in vitro

Abraham

Venter

Lues

et al. 2009

International Journal of Antimicrobial Agents

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“…The authors further sufficed with the direct correlation between compound quantity and the total ion count expressed in this paper as MS signal (Venter et al, 2006a(Venter et al, , 2006b. A procedure error of less than 5% was upheld, confirming the report by Bohin et al (2005).…”

Section: Resultssupporting

confidence: 65%

The exopolysaccharide (EPS) ultra structure ofStaphylococcus aureus: changes occurring in EPS resulting from exposure to physical and chemical food preservation practises in South Africa

et al. 2009

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-This paper aimed to investigate the ultra structure of Staphylococcus aureus EPS and describe the changes that occurred following exposure to pasteurisation, sodium benzoate preservation and treatment with a bacteriostatic hand cleanser. Mass spectrometry was employed to identify and monitor the presence and distribution of certain saccharides and fatty acid constituents. Fatty acid and saccharide profiles demonstrated that the ultra structure was subject to change and diversification as a result of exposure. Components generally associated with Gram-negative LPS, 2-keto-3-deoxyoctulosonic acid (KDO) and β-hydroxy fatty acids, were also identified in the EPS of S. aureus.

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“…Advances in these areas will likely extend the current foundation upon which the studies herein were built. For a review of these methods, the reader should consult references [70][71][72][73][74][75][76][77][78][79] for a treatment of the analytical methods and references [80][81][82][83][84][85][86][87] for synthetic methods used to study KDN and KDN-containing glycoconjugates.…”

Section: Development Of Analytical and Synthetic Methods For The Studmentioning

confidence: 99%

KDN (Deaminated neuraminic acid): Dreamful past and exciting future of the newest member of the sialic acid family

2006

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KDN is an abbreviation for 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid, and its natural occurrence was revealed in 1986 by a research group including the present authors. Since sialic acid was used as a synonym for N-acylneuraminic acid at that time, there was an argument if this deaminated neuraminic acid belongs to the family of sialic acids. In this review, we describe the 20 years history of studies on KDN (KDNology), through which KDN has established its position as a distinct member of the sialic acid family. These studies have clarified that: (1) KDN occurs widely among vertebrates and bacteria similar to the occurrence of the more common sialic acid, N-acetylneuraminic acid (Neu5Ac), but its abundant occurrence in animals is limited to lower vertebrates. (2) KDN is found in almost all types of glycoconjugates, including glycolipids, glycoproteins and capsular polysaccharides. (3) KDN residues are linked to almost all glycan structures in place of Neu5Ac. All linkage types known for Neu5Ac; alpha2,3-, alpha2,4-, alpha2,6-, and alpha2,8- are also found for KDN. (4) KDN is biosynthesized de novo using mannose as a precursor sugar, which is activated to CMP-KDN and transferred to acceptor sugar residues. These reactions are catalyzed by enzymes, some of which preferably recognize KDN, but many others prefer Neu5Ac to KDN. In addition to these basic findings, elevated expression of KDN was found in fetal human red blood cells compared with adult red blood cells, and ovarian tumor tissues compared with normal controls. KDNase, an enzyme which specifically cleaves KDN-linkages, was discovered in a bacterium and monoclonal antibodies that specifically recognize KDN residues in KDNalpha2,3-Gal- and KDNalpha2,8-KDN-linkages have been developed. These have been used for identification of KDN-containing molecules. Based on past basic studies and variety of findings, future perspective of KDNology is presented.

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GC/MS identification and quantification of constituents of bacterial lipids and glycoconjugates obtained after methanolysis as heptafluorobutyrate derivatives

Cited by 26 publications

References 30 publications

Influence of selected antimicrobials on the viability, endotoxicity and lipopolysaccharide composition of Pseudomonas aeruginosa in vitro

Influence of selected antimicrobials on the viability, endotoxicity and lipopolysaccharide composition of Pseudomonas aeruginosa in vitro

The exopolysaccharide (EPS) ultra structure ofStaphylococcus aureus: changes occurring in EPS resulting from exposure to physical and chemical food preservation practises in South Africa

KDN (Deaminated neuraminic acid): Dreamful past and exciting future of the newest member of the sialic acid family

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