GC-MS-FID and potentiation of the antibiotic activity of the essential oil of Baccharis reticulata (ruiz &amp; pav.) pers. and α-pinene

Freitas, Priscilla Ramos; Araújo, Ana Carolina Justino de; Barbosa, Cristina Rodrigues dos Santos; Muniz, Débora Feitosa; Silva, Ana Cristina Albuquerque da; Rocha, Janaína Esmeraldo; Oliveira-Tintino, Cícera Datiane de Morais; Ribeiro-Filho, Jaime; Silva, Luiz Everson da; Confortin, Camila; Amaral, Wanderlei do; Deschamps, Cícero; Barbosa-Filho, José Maria; Lima, Natanael Teles Ramos de; Tintino, Saulo Relison; Coutinho, Henrique Douglas Melo

doi:10.1016/j.indcrop.2020.112106

Cited by 24 publications

(15 citation statements)

References 27 publications

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“…On the other hand, the monoterpene hydrocarbons viz ., β -myrcene, α -pinene, and limonene are generally known to exert less antibacterial effects in comparison to oxygenated monoterpenes and phenolics [ 35 ] and might act to synergize the main action of cineole and eugenol in Pimenta oil, thus accounting for the superior antibiofilm effect of the oil compared to eugenol standard tested alone ( Figure 1 ). The lipophilic nature of terpene hydrocarbons greatly affects bacterial membrane permeability and might improve uptake of E.O components into bacterial cells enhancing its penetration into the bacterial biofilms and thus exerting its antimicrobial and antibiofilm action and further potentiating the effect of other concomitant antibiotics [ 36 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

et al. 2020

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Bacterial biofilm contributes to antibiotic resistance. Developing antibiofilm agents, more favored from natural origin, is a potential method for treatment of highly virulent multidrug resistant (MDR) bacterial strains; The potential of Pimenta dioica and Pimenta racemosa essential oils (E.Os) antibacterial and antibiofilm activities in relation to their chemical composition, in addition to their ability to treat Acinetobacter baumannii wound infection in mice model were investigated; P. dioica leaf E.O at 0.05 µg·mL−1 efficiently inhibited and eradicated biofilm formed by A. baumannii by 85% and 34%, respectively. Both P. diocia and P. racemosa leaf E.Os showed a bactericidal action against A. baumanii within 6h at 2.08 µg·mL−1. In addition, a significant reduction of A. baumannii microbial load in mice wound infection model was found. Furthermore, gas chromatography mass spectrometry analysis revealed qualitative and quantitative differences among P. racemosa and P. dioica leaf and berry E.Os. Monoterpene hydrocarbons, oxygenated monoterpenes, and phenolics were the major detected classes. β-Myrcene, limonene, 1,8-cineole, and eugenol were the most abundant volatiles. While, sesquiterpenes were found as minor components in Pimenta berries E.O; Our finding suggests the potential antimicrobial activity of Pimenta leaf E.O against MDR A. baumannii wound infections and their underlying mechanism and to be further tested clinically as treatment for MDR A. baumannii infections.

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Section: Resultsmentioning

confidence: 99%

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

et al. 2020

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“…Different studies confirmed the antibacterial properties of α-pinene [ 52 ]. Freitas et al [ 53 ] reported that α-pinene has antibacterial and antibiotic-modulating activities against S. aureus; it also increases the activity of norfloxacin against E. coli and norfloxacin and gentamicin against S. aureus . Furthermore, Hippeli et al [ 54 ] described an anti-inflammatory potential of P. mugo EOs and its main compound α-pinene, while Cole et al [ 55 ] showed anti-proliferative activity on the MCF-7 cell line.…”

Section: Discussionmentioning

confidence: 99%

Liquid and Vapor Phase of Four Conifer-Derived Essential Oils: Comparison of Chemical Compositions and Antimicrobial and Antioxidant Properties

et al. 2021

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In this study, the chemical composition of the vapor and liquid phase of Pinus cembra L., Pinus mugo Turra, Picea abies L., and Abies Alba M. needles essential oils (EOs) was investigated by Headspace-Gas Chromatography/Mass Spectrometry (HS-GC/MS). In the examined EOs, a total of twenty-eight components were identified, most of which belong to the monoterpenes family. α-Pinene (16.6–44.0%), β-pinene (7.5–44.7%), limonene (9.5–32.5%), and γ-terpinene (0.3–19.7%) were the most abundant components of the liquid phase. Such major compounds were also detected in the vapor phase of all EOs, and α-pinene reached higher relative percentages than in the liquid phase. Then, both the liquid and vapor phases were evaluated in terms of antibacterial activity against three Gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens, and Acinetobacter bohemicus) and two Gram-positive bacteria (Kocuria marina and Bacillus cereus) using a microwell dilution assay, disc diffusion assay, and vapor phase test. The lowest Minimum Inhibitory Concentration (MIC) (13.28 mg/mL) and Minimal Bactericidal Concentration (MBC) (26.56 mg/mL) values, which correspond to the highest antibacterial activities, were reported for P. abies EO against A. bohemicus and for A. alba EO against A. bohemicus and B. cereus. The vapor phase of all the tested EOs was more active than liquid phase, showing the inhibition halos from 41.00 ± 10.15 mm to 80.00 ± 0.00 mm for three bacterial strains (A. bohemicus, K. marina, and B. cereus). Furthermore, antioxidant activities were also investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3- ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays, and a concentration-dependent antioxidant capacity for all EOs was found. P. mugo EO showed the best antioxidant activity than the other Pinaceae EOs. The four Pinaceae EOs could be further investigated for their promising antibacterial and antioxidant properties, and, in particular, α-pinene seems to have interesting possibilities for use as a novel natural antibacterial agent.

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“…These monoterpenes have been found as major constituents of essential oils obtained from a wide variety of aromatic plants [8]. Accordingly, the essential oil of the leaves of Aloysia gratissima and Baccharis reticulata, which have 1,8-cineole, germacrene D, α-pinene, β-caryophyllene, and β -pinene as major constituents, demonstrated bactericidal activity against both Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacterial strains [9,10].…”

Section: Introductionmentioning

confidence: 99%

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

et al. 2020

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Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil Ocotea odorífera (EOOO) and safrole against efflux pump (EP)-carrying strains. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial and antibiotic-modulating activities of the EOOO and safrole against resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were analyzed through the broth microdilution method. The EP-inhibiting potential of safrole in association with ethidium bromide or antibiotics was evaluated using the S. aureus 1199B and K2068 strains, which carry genes encoding efflux proteins associated with antibiotic resistance to norfloxacin and ciprofloxacin, respectively. A reduction in the MIC of ethidium bromide or antibiotics was used as a parameter of EP inhibition. The phytochemical analysis identified 16 different compounds in the EOOO including safrole as the principal constituent. While the EOOO and safrole exerted clinically relevant antibacterial effects against S. aureus only, they potentiated the antibacterial activity of norfloxacin against all strains evaluated by our study. The ethidium bromide and antibiotic assays using the strains of S. aureus SA1119B and K2068, as well as molecular docking analysis, indicated that safrole inhibits the NorA and MepA efflux pumps in S. aureus. In conclusion, Ocotea odorifera and safrole presented promising antibacterial and antibiotic-enhancing properties, which should be explored in the development of drugs to combat antibacterial resistance, especially in strains bearing genes encoding efflux proteins.

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GC-MS-FID and potentiation of the antibiotic activity of the essential oil of Baccharis reticulata (ruiz & pav.) pers. and α-pinene

Cited by 24 publications

References 27 publications

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

Pimenta Oil as a Potential Treatment for Acinetobacter baumannii Wound Infection: In Vitro and In Vivo Bioassays in Relation to Its Chemical Composition

Liquid and Vapor Phase of Four Conifer-Derived Essential Oils: Comparison of Chemical Compositions and Antimicrobial and Antioxidant Properties

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

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