GC/MS determination of guanidinoacetate and creatine in urine: A routine method for creatine deficiency syndrome diagnosis

Nasrallah, Fahmi; Feki, Moez; Briand, Gilbert; Kaabachi, Naziha

doi:10.1016/j.clinbiochem.2010.08.004

Cited by 15 publications

(8 citation statements)

References 29 publications

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“…Almeida et al 2004;Arias et al 2006;Bodamer et al 2001;Nasrallah et al 2010;Valongo et al 2004;Marescau et al 1992;Verhoeven et al 2005;Joncquel-Chevalier Curt et al 2013). In urine, the creatine/ creatinine (Cr/Crn) ratio and guanidinoacetate levels decrease with age.…”

mentioning

confidence: 99%

X‐linked creatine transporter deficiency: clinical aspects and pathophysiology

Kamp

Mancini

Salomons

2014

J of Inher Metab Disea

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Creatine transporter deficiency was discovered in 2001 as an X-linked cause of intellectual disability characterized by cerebral creatine deficiency. This review describes the current knowledge regarding creatine metabolism, the creatine transporter and the clinical aspects of creatine transporter deficiency. The condition mainly affects the brain while other creatine requiring organs, such as the muscles, are relatively spared. Recent studies have provided strong evidence that creatine synthesis also occurs in the brain, leading to the intriguing question of why cerebral creatine is deficient in creatine transporter deficiency. The possible mechanisms explaining the cerebral creatine deficiency are discussed. The creatine transporter knockout mouse provides a good model to study the disease. Over the past years several treatment options have been explored but no treatment has been proven effective. Understanding the pathogenesis of creatine transporter deficiency is of paramount importance in the development of an effective treatment.

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mentioning

confidence: 99%

X‐linked creatine transporter deficiency: clinical aspects and pathophysiology

Kamp

Mancini

Salomons

2014

J of Inher Metab Disea

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“…Urinary creatinine was determined using the Jaffe method [17]. Urinary Cr was determined by mass spectrometry coupled gas chromatography (GC/ MS) as described by Nasrallah et al [21]. Briefly, 100 µL of urine is mixed with 100 µL of 2-phenylbutyric acid (internal standard), 50 µL of saturated sodium bicarbonate solution, 600 µL of toluene and 50 µL of hexafluoro-acetylacetone.…”

Section: Methodsmentioning

confidence: 99%

Urinary Creatine at Rest and After Repeated Sprints in Athletes: A Pilot Study

Bezrati-Benayed¹,

Nasrallah²,

Feki³

et al. 2014

Biol Sport

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Creatine plays a key role in muscle function and its evaluation is important in athletes. In this study, urinary creatine concentration was measured in order to highlight its possible significance in monitoring sprinters. The study included 51 sprinters and 25 age- and sex-matched untrained subjects as a control group. Body composition was measured and dietary intake estimated. Urine samples were collected before and after standardized physical exercise. Creatine was assessed by gas chromatography mass spectrometry. Basal urinary creatine (UC) was significantly lower in sprinters than controls (34±30 vs. 74±3 µmol/mmol creatinine, p < 0.05). UC was inversely correlated with body mass (r = −0.34, p < 0.01) and lean mass (r = −0.30, p < 0.05), and positively correlated with fat mass (r = 0.32, p < 0.05). After acute exercise, urinary creatine significantly decreased in both athletes and controls. UC is low in sprinters at rest and further decreases after exercise, most likely due to a high uptake and use of creatine by muscles, as muscle mass and physical activity are supposed to be greater in athletes than untrained subjects. Further studies are needed to test the value of urinary creatine as a non-invasive marker of physical condition and as a parameter for managing Cr supplementation in athletes.

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“…It is urgent and beneficial to develop a sensitive method for injury detection in early stage. Due to the sensitive characteristics of the endogenous substances with low molecular weights, they were usually selected as the biomarkers for monitoring different diseases or exploring the activity mechanism [13][14][15][16]. In the present study, a total of ten sensitive biomarkers for liver and kidney injury were summarized and selected according to the relative reports, including hippuric acid (HIP), phenylacetylglycine (PA), 5-oxoproline (5-OP), cholic acid (CA), taurine (TA), indoleacetic acid (IA), 3-indoxyl sulfate (3-IS), guanidinosuccinic acid (GSA), guanidinoacetic acid (GAA), and uric acid (UA).…”

Section: Liquid Chromatography 3937mentioning

confidence: 99%

“…To date, although some approaches, such as CE , GC , HPLC–UV , LC–MS , have been reported to determine one or several of the biomarkers, separately, none of comprehensive methods were reported to determine all of them for simultaneous monitoring of liver and kidney injury, nor applications to injury induced by EPR. Besides, CE may lack robustness for routine tests of biosamples.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of biomarkers of liver and kidney injury in serum and urine using ultra-fast liquid chromatography with tandem mass spectrometry coupled with a hydrophilic interaction chromatography column: Application to monitor injury induced by E

Zhang

Jiang

et al. 2016

J. Sep. Science

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Fast and sensitive monitoring of drug-induced liver and kidney injury in early stage is beneficial. An ultrafast liquid chromatography with tandem mass spectrometry assay was developed and validated to simultaneously determine ten endogenous biomarkers in serum and urine, including hippuric acid, phenylacetylglycine, 5-oxoproline, cholic acid, taurine, indoleacetic acid, 3-indoxyl sulfate, guanidinosuccinic acid, guanidinoacetic acid and uric acid. A CAPCELL CORE PC column (2.1 × 150 mm, 2.7 μm) was adopted for analytes separation. Gradient elution was performed with acetonitrile and water containing 5 mM ammonium acetate. Simple protein precipitation was applied in sample preparation. Good linearities were achieved with all the regression coefficients above 0.9911. Accuracy was 2.9-14.2% in serum and 4.1-14.6% in urine. The mean recovery was above 70% with acceptable matrix effects. The method was applied to monitor injury induced by Euphorbiae pekinensis Radix with a subacute rats model. All the biomarkers showed obvious concentration changes during the injury period. Furthermore, several biomarkers showed significant changes in earlier stage when compared with the current clinical serum bio-parameters. The method might be helpful for early diagnosis of drug induced liver and kidney injury in clinical after tested on more drugs.

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GC/MS determination of guanidinoacetate and creatine in urine: A routine method for creatine deficiency syndrome diagnosis

Cited by 15 publications

References 29 publications

X‐linked creatine transporter deficiency: clinical aspects and pathophysiology

X‐linked creatine transporter deficiency: clinical aspects and pathophysiology

Urinary Creatine at Rest and After Repeated Sprints in Athletes: A Pilot Study

Quantitative analysis of biomarkers of liver and kidney injury in serum and urine using ultra-fast liquid chromatography with tandem mass spectrometry coupled with a hydrophilic interaction chromatography column: Application to monitor injury induced by E

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