GC-MS-Based Metabolomics Analysis of Prawn Shell Waste Co-Fermentation by Lactobacillus plantarum and Bacillus subtilis

Abstract

GC-MS-based metabolomics were used to investigate metabolic changes in prawn shell waste during fermentation. Microbial strains Lactobacillus plantarum and Bacillus subtilis were co-fermented in a shake flask comprising of 5% (w/v) prawn shell waste and 20% (w/v) glucose as a carbon source. Analysis of the prawn shell waste fermentation showed a total of 376 metabolites detected in the culture supernatant, including 14 amino acids, 106 organic acids, and 90 antimicrobial molecules. Results show that the liquid fraction of the co-fermentation is promising for harvesting valuable metabolites for probiotics application.

The Antibacterial and Antibiofilm Properties of Different Strains of Lactobacillus spp. Isolated from Traditional Kefir Dough on Oral Pathogens with Real Time PCR

“…In 2020, the derivatives of Nonadecanoic acid, benzoic acid, hexadecanoic acid and pyrrol were separated from the Lactobacillus plantarum. The results of this research are in line with those reported here in [30]. We extracted LAB from ke r dough and tested its antibacterial properties against S. mutans and P. gingivalis bio lms.…”