Gc-MS Based Chemical Profiling and Evaluation of Antioxidant Potential of Leaves and Stems of Alternanthera Sessilis Red From Sabah, Malaysia

Abstract

Objectives: The aim of the current study was to perform phytochemical screening and determination of total phenolics, total flavonoids and antioxidant activity of various crude extracts of Alternanthera sessilis red leaves.Methods: Determination of antioxidant nature of Alternanthera sessilis red was carried out by DPPH radical scavenging method. GC-MS analysis of various crude extracts resulted in the presence of different types of low and high molecular weight compounds consisting of carbohydrates, fatty acid and vitamins.Results: Among all the extracts, methanol extracts exhibited higher DPPH radical scavenging activity with IC50 value of 0.183 mg/ml. BHT used as the positive control showed IC50 value of 0.089 mg/mL. The results suggested that the crudes extracts of Alternanthera sessilis red exhibit moderate antioxidant activity. The total phenolic content (279.8 ± 0.02 mg GAE/g) and total flavonoid content (250.7 ± 0.03 mg QE/g) in methanol was estimated higher as compared to other solvents.Conclusion: The total flavonoid content and total phenolic content were found to be higher in methanolic extract followed by ethyl acetate and hexane extracts.

The Genus Alternanthera: Phytochemical and Ethnopharmacological Perspectives

“…ex DC. : Borah and the team had used 90% methanolic, 70% acetone, 80% ethanolic extracts of leaves and stems ( Borah et al, 2011 ); Chai and the team had used hexane, chloroform, ethyl acetate, butanolic, and aqueous fractions of leaves and callus methanol extracts ( Chai et al, 2016 ); Sharma and the team 30% hydroethanolic extract of the whole plant ( Sharma et al, 2013 ); Khan and the team had used separate Methanolic and hexane extracts of leaves and stems ( Khan et al, 2018 ); Azizah and the team had used ethanolic and aqueous extracts of aerial parts ( Azizah et al, 2015 ); Muniandy and the team had used 90% hydroethanolic extract of stem ( Muniandy et al, 2018b ); Othman and the team had used ethanolic and aqueous extracts of aerial parts ( Othman et al, 2016 ); Tiwari and the team had used juice ( Tiwari et al, 2013 ); Rajamurugan and the team had used ethanolic extract of leaves ( Rajamurugan et al, 2013 ); Jain and the team had used methanolic extract of leaves ( Jain et al, 2016 ); Suganya and the team had used aqueous extract of leaves and stems ( Suganya et al, 2019 ); Mohd Hazli and the team had used hexane, ethyl acetate, ethanolic, and aqueous extracts of leaves and stem ( Mohd Hazli et al, 2019 ); Niraimathi and the team had used silver nanoparticles from aqueous extract of leaves ( Niraimathi et al, 2013 ); Yap and the team had used 100% ethanolic, 70% ethanolic, 80% methanolic, ethyl acetate, and aqueous extracts of the whole plant ( Yap et al, 2019 ); Kota and the team had used petroleum ether, ethyl acetate, chloroform, and methanolic extract of leaves ( Kota et al, 2017 ); Sundar and the team had used petroleum ether and methanolic extracts of leaves ( Sundar et al, 2019 ); Pathak and the team had used n-hexane and methanolic extracts of aerial parts ( Pathak et al, 2020 ); Khan and the team had used the volatile oil of leaves and flowers ( Khan et al, 2016 ); while Salvador and the team had used ethanolic extract and its four fractions; Acacetin 8-c-[ α -L-rhamnopyranoyl-(1→2)- β -D-glucopyranoside]; 2″- O -α-L-rhamnopyranosyl-vitexin; 2″- O - β -D-glucopyranosyl vitexin and Vitexin ( Salvador et al, 2006 ). Results from these researches left no doubt in the credibility and applicability of Alternanthera sessilis (L.) R.Br.…”

Amaranthaceae plants of Israel and Palestine: Medicinal activities and unique compounds

“…246 The following references reported chemical compositions (some are very detailed) of known natural products. 233,234,[247][248][249][250] Special method for detection and quantification of gallic acid in the plant. 251 Very extensive work was invested in this research, especially in spectroscopic characterization of the reported compound.…”

Untitled

“…Preparation of organic crude extracts was based on Khan et al, with some minor modification 20 . 300 g of the powdered sample was distributed equally among three beakers, having 400 ml methanol.…”