GC–MS analysis of volatile compounds of  Perilla frutescens  Britton var.  Japonica  accessions: Morphological and seasonal variability

Thujone is a volatile monoterpene ketone of plant origin which is produced by several plants that are frequently used for flavoring foods and beverages. The use of thujone and thujone-containing plant parts for human consumption is currently regulated by the European Parliament and Council and the European Medicines Agency. The best known neurotoxic effects are connected to the GABA-gated chloride channel, where a-thujone is a modulator roughly two to three times as potent as the b isomer. Based primarily on in vitro experiments, genotoxicity and carcinogenic properties of thujones have also been detected in parallel with antimutagenic and immune-modulatory effects. Some of the controversial effects seem to be strongly dose-dependent. Data on antidiabetic and antimicrobial activities of thujones may show new ways to use them. This review also describes the main steps of the biosynthetic route of thujones and their occurrence in the plant kingdom. The accumulation of these compounds seems to be more abundant in some plant families (e.g. Asteraceae or Cupressaceae) than in others. Four species (Artemisia absinthium, Salvia

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“…Seidler-Ło_ zykowska et al 2017; Németh-Zámboriné et al 2019); or Perilla frutescens (e.g. Chauhan et al 2013;Ghimire et al 2017;Yu et al 1997).…”

Section: Occurence Of Thujone In Aromatic Plants Chemotaxonomic Aspectsunclassified

Thujone, a widely debated volatile compound: What do we know about it?

Németh

Nguyen

2020

Phytochem Rev

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“…The green-leaf chemotypes show only trace amount of anthocyanin type 261 compounds among all polyphenol compounds encountered [12]. 262 [36,37,38] based on the main components of the EOs, different chemotypes has been described in perilla such as perillaketone (PK) (isoegomaketone), perillaldehyde (PA), elsholtziaketone (EK), citral (C), phenylpropanoids (PP) (myristicin, dillapiole, elemicin), perillene (PL), beta-caryophyllene, myristicine (MT), limonene, piperitenone (PT). EOs have known to posses various bioactivities including antibacterial, antiviral, antifungal, antiinflammatory, antimutagenic, anticarcinogenic, antidiabetic, antiprotozoal and antioxidants as well [35].…”

Section: Phenolic Compounds Flavonoids Anthocyaninsmentioning

confidence: 99%

Ethnomedicinal, Phytochemical and Pharmacological Investigations of <em>Perilla frutescens</em> (L.) Britt<strong> </strong>

Ahmed

2018

Preprint

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Perilla frutescens (L.) Britt. (PF) is an annual herbal medicinal, aromatic, functional food and ornamental plant that belongs to the mint family, Lamiaceae. The origin of perilla traces back to East Asian countries (China, Japan, Korea, Taiwan, Vietnam and India), where it has been used as a valuable source of culinary and traditional medicinal uses. Leaves, seeds and stems of P. frutescens are used for various therapeutic applications in folk medicine. In the absence of comprehensive review regarding all aspects of perilla, thus this review aims to present an overview pertaining to the botanical drug, ethnobotany, phytochemistry and biological activity. It was found that the taxonomic classification of perilla species is quite confused, and the number of species is vague. Perilla has traditionally been prescribed to treat depression-related disease, anxiety, asthma, chest stuffiness, vomiting, cough, cold, flus, phlegm, tumour, allergy, intoxication, fever, headache, stuffy nose, constipation, abdominal pain, indigestion, analgesic, anti-abortive agent, and sedative. Until now, 271 natural molecules have been identified in perilla organs including; polyphenols, flavonoids, essential oils, triterpenes, carotenoids, phytosterols, fatty acids, tocopherols and policosanols. In addition to solvent extracts, these individual compounds (rosmarinic acid, perillaldehyde, luteolin, apigenin, tormentic acid, isoegomaketone) have attracted researchers' interest for pharmacological properties. Its bioactivity showed antioxidant, antimicrobial, anti-allergic, antidepressant, anti-inflammatory, anticancer, neuroprotection activity. Although the results are promising in preclinical studies (in vitro and in vivo) as well, clinical studies are insufficient, therefore further study needs to be done to validate its therapeutic effects and to ensure its safety and efficacy.

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“…Perilla ( Perilla frutescens ) is a spicy vegetable widely cultivated in Asian countries [ 23 , 24 , 25 ]. The leaves and seeds of perilla have long been used as traditional Chinese medicines to treat various conditions, such as the common cold, headaches and coughing [ 23 , 24 , 25 ]. Perilla leaves produce a wide array of phenolics and terpenes [ 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…The leaves and seeds of perilla have long been used as traditional Chinese medicines to treat various conditions, such as the common cold, headaches and coughing [ 23 , 24 , 25 ]. Perilla leaves produce a wide array of phenolics and terpenes [ 23 , 24 , 25 , 26 ]. Volatile compounds in perilla leaves are mainly monoterpenes (perillaldehydes and perillaketones) and phenylpropenes (myristicin, elemicin and dillapiole) [ 23 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Perilla leaves produce a wide array of phenolics and terpenes [ 23 , 24 , 25 , 26 ]. Volatile compounds in perilla leaves are mainly monoterpenes (perillaldehydes and perillaketones) and phenylpropenes (myristicin, elemicin and dillapiole) [ 23 , 25 , 26 ]. The hydrophilic secondary metabolites in perilla leaves include rosmarinic acid and its derivatives, and flavonoids including apigenin, luteolin and chrysoeriol [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Biochemical Characterization of a Flavonoid O-methyltransferase from Perilla Leaves and Its Application in 7-Methoxyflavonoid Production

Park

Lee

et al. 2020

Molecules

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Methylation is a common structural modification that can alter and improve the biological activities of natural compounds. O-Methyltransferases (OMTs) catalyze the methylation of a wide array of secondary metabolites, including flavonoids, and are potentially useful tools for the biotechnological production of valuable natural products. An OMT gene (PfOMT3) was isolated from perilla leaves as a putative flavonoid OMT (FOMT). Phylogenetic analysis and sequence comparisons showed that PfOMT3 is a class II OMT. Recombinant PfOMT3 catalyzed the methylation of flavonoid substrates, whereas no methylated product was detected in PfOMT3 reactions with phenylpropanoid substrates. Structural analyses of the methylation products revealed that PfOMT3 regiospecifically transfers a methyl group to the 7-OH of flavonoids. These results indicate that PfOMT3 is an FOMT that catalyzes the 7-O-methylation of flavonoids. PfOMT3 methylated diverse flavonoids regardless of their backbone structure. Chrysin, naringenin and apigenin were found to be the preferred substrates of PfOMT3. Recombinant PfOMT3 showed moderate OMT activity toward eriodictyol, luteolin and kaempferol. To assess the biotechnological potential of PfOMT3, the biotransformation of flavonoids was performed using PfOMT3-transformed Escherichia coli. Naringenin and kaempferol were successfully bioconverted to the 7-methylated products sakuranetin and rhamnocitrin, respectively, by E. coli harboring PfOMT3.

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GC–MS analysis of volatile compounds of Perilla frutescens Britton var. Japonica accessions: Morphological and seasonal variability

Cited by 47 publications

References 52 publications

Thujone, a widely debated volatile compound: What do we know about it?

Thujone, a widely debated volatile compound: What do we know about it?

Ethnomedicinal, Phytochemical and Pharmacological Investigations of <em>Perilla frutescens</em> (L.) Britt<strong> </strong>

Biochemical Characterization of a Flavonoid O-methyltransferase from Perilla Leaves and Its Application in 7-Methoxyflavonoid Production

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