GC-MS Analysis of Ethanol Extract of Taxithelium napalense (Schwaerg) Broth along with its alpha-Glucosidase Inhibitory Activity

Abstract

Tatipamula et al.: GC-MS analysis of Taxithelium napalense Considering the antidiabetic potentiality of the moss Taxithelium napalense, the present study was undertaken to explore the chemical constituents that are present in this species. The entire moss Taxithelium napalense was extracted by ethanol and the extract obtained was subjected to gas chromatography-mass spectrometry. Sixty nine compounds were identifi ed, which are reported for the fi rst time from this species. From the gas chromatography-mass spectrometry analysis, it was determined that the octadecanoic acid methyl ester was the chief component present in Taxithelium napalense. In addition, the total phenolic and fl avonoid contents of ethanol extract of Taxithelium napalense were found to be 101.43±0.38 mg Q/g and 229.73±3.07 mg GA/g, respectively. Additionally, the IC 50 of the ethanol extract of Taxithelium napalense on α-glucosidase was found to be 34.5 μg/ml, while acarbose value was 29.5 μg/ml. This is the fi rst report on the chemical investigation of the moss Taxithelium napalense.

Role of Ethanolic Extract of Microdus brasiliensis (Duby) Ther. in Protecting Survival Rate in Sepsis: An In vivo Study

“…The total phenolic content of Mb was established through the Folin-Ciocalteu reagent [14] . Initially, five hundred microliters of Mb were mixed with 5 ml of Folin-Ciocalteu reagent 10 % (v/v) in distilled water, diluted and 4 ml of sodium bicarbonate solution (1 M) was added.…”

HPTLC Analysis, Antioxidant and Antidiabetic activities of Ethanol Extract of Moss Fissidens grandiflora

“…The total flavonoid content [22] of the extracts was determined using aluminium chloride spectrophotometric method, in which AlCl 3 forms a complex with hydroxyl groups of flavonoids in the test sample. To the extract (1 mg/ml) or standard quercetin solution (3.125, 6.25, 12.5, 25, 50, 100 µg/ml) 3 ml of methanol, 1 ml of 2 % AlCl 3 solution, 200 µl of 1 M potassium acetate was added and the volume was made up to 10 ml with distilled water and incubated for 60 min at room temperature.…”

“…The assay of α-glucosidase inhibitory activity [22] was performed in a triplicate. A stock solution of α-glucosidase acetone powder from rat intestine acetone powder was made prepared (1.0 mg/ml in 10 mM phosphate buffer, pH 6.8, diluted 40-fold with the same buffer) and 2.0 µl of this solution was mixed with 20 µl of the samples of different concentrations (25, 50, 75 and 100 µg/ml dissolved in DMSO) and 100 µl of 50 mM phosphate buffer (pH 6.8) in 96 well microplates and incubated for 5 min at 37°.…”

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“…For example, solvent removal may cause modifications which will influence the particle shape. [19] DLS, also known as PCS or quasi-elastic light scattering, records the variation in the intensity of scattered light on the microsecond time scale. This variation results from interference of light scattered by individual particles under the influence of Brownian motion and is quantified by compilation of an autocorrelation function.…”