GC-MS Analysis of Essential Oil Composition and Insecticidal Activity of Syzygium aromaticum against Callosobruchus Maculatus of Chickpea

Abstract

The beetle Callosobruchus maculatus (Chrysomelidae: Bruchinae) is a destructive pest of stored chickpea seeds. The use of plant materials rather than conventional pesticides to control these pests is a promising alternative to chemical insecticides. The present study evaluated the insecticidal activity of clove essential oils by three different methods on Callosobruchus maculatus. The oils were obtained by hydro-distillation with a yield of 6.3 ± 0.23% and analyzed by GC-MS. In total, 65 compounds representing 99.92% of the oil were identified and the main components 80.26% Eugenol, 9.62% Eugenyl acetate, 6.74% β-Caryophyllene and 1.14% C-Humulene. The results of the insecticidal activity of S. aromaticum on C. maculatus performed by inhalation shows that a significant mortality was observed at a concentration of 20 μl/l against insects exposed at 24 hours (6.67%), 48 hours (36.67%) and 72 hours (100%).Contact test results are lower than those produced by inhalation. Essential oil decreased oviposition and fully suppressed adult growth at the highest dose of 20 μl/l of air per contact and inhalation test. The results of the present research have shown that EO had a high effectiveness against Callosobruchus maculatus, and in another hand these results can be used as a source of natural product to avoid the contamination of chickpea seeds, and also as an alternative to chemical products.

Antioxidant, Antimicrobial, and Insecticidal Properties of a Chemically Characterized Essential Oil from the Leaves of Dittrichia viscosa L.

“…The test was performed according to the protocol described by Allali and co-workers [ 31 ], where a small piece of cotton was placed into the bottom of a glass flask, and 10 C. maculatus individuals were placed in each flask. EOD was added to the cotton at different doses (1, 5, 10, 20 μL/L of air).…”

Chemical Composition and Antifungal, Insecticidal and Repellent Activity of Essential Oils From Origanum compactum Benth. Used in the Mediterranean Diet

“…This EO reduced significantly the life span of C. maculatus adult bruchids, even at the lowest doses used. This high efficiency resulted in a low value of the LC 50 , 5.53 μL/100 g (contact test) that might be induced by the action of major compounds of these EO ( Allali et al, 2021 ).…”

“…Medicinal and aromatic plants are important sources of EO that find applications in various areas of life. EO is mainly used as a food flavoring but can be successfully used for various non-food applications, as it exhibits many biological properties, including antifungal, antimicrobial, antioxidant, and insecticidal activity ( Mssillou et al, 2020 ; Allali et al, 2021 ; El Moussaoui et al, 2021 ).…”