GC/MS Analysis and Potential Cytotoxic Activity of Haplophyllum tuberculatum Essential Oils Against Lung and Liver Cancer Cells

Abstract

Background: Haplophyllum tuberculatum is a plant belongs to family rutacee. It is rich in volatile oils, fixed oils, alkaloids, and furanocoumarins. It is well known for the huge number of folkloric uses in middle east. Objective: The aim of this study is to establish the chemical composition of the essential oils of Libyan H. tuberculatum and to investigate their cytotoxic potentialities.Materials and Methods: The essential oils of the aerial parts and flowers of H. tuberculatum growing in Libya were prepared by hydrodistillation. GC/MS analyses were performed on a Shimaduz capillary gas chromatograph (GC 17A ver.3) instrument directly coupled to mass spectrometer-MS QP5050A. Oil A and F of H. tuberculatum at different concentrations (0-50 µg/ml) in DMSO were tested for cytotoxicity against human tumor cell lines. Results: Oil yield was found 0.4 and 1.5 (v/w %) on dry weight basis respectively. GC/MS analysis resulted in identification of total 35 compounds. 15 compounds were common to both oils. oil A of H. tuberculatum exhibited antitumor activities against. liver carcinoma

<i>Haplophyllum tuberculatum</i> (Forssk.) A. Juss Essential Oils: Seasonal Contents Variation, Bioactivity of the Traditionally-favored, High-yield and Frequent-use Summer Season Oil

“…The findings also indicated remarkable cytotoxic effects of the oil against the MCF 7 (breast cancer, IC 50 value of 8.91 μg/mL as compared to the 2.4 μg/mL for the doxorubicin) , HEP2, IC 50 value of 7.9 μg/mL) , and HCT116 (IC 50 value of 6.3 μg/mL compared to 1.9 μg/mL for the doxorubicin) cell lines. These finding also corroborated the previous study, which exhibited the cytotoxic potential of the H. tuberculatum oil against the HEPG2 and H1299 35) cells.…”

Phytochemical Analysis Using cLC-DAD, Nutritional Importance and Assessment of Antioxidant, Antidiabetic and Anticholinesterase Activities of Ruta tuberculata Forssk Organic Extracts

“…While no reports concerning the phytochemical composition and the pharmacological proprieties of the acetonic extract were published for all species of genus Ruta, as the best of the author's acknowledgment. Indeed, most studies of Ruta plants focused mostly on their alkaloid derivatives and essential oil compounds [12,20,22,39], whereas their phenolic contents and pharmacotherapies of them were rarely investigated [17,19,21,40]. That heightened our desire to characterize for the first time the individual phenolic profiles of the EtOAc and acetonic extracts of R. tubercultata aerial parts and to investigate their pharmacological properties.…”

Effect of Microfluidization on the Volatiles and Antibacterial, Antifungal, and Cytotoxic Activities of Algerian Satureja hortensis L. Oil-Loaded Nanoemulsions: In Vitro and In Silico Study

“…Studying the chemical composition of essential oil extracted from the Algerian cultivar of S. hortensis L. (Table 1) allows us to know its potential use in various applications such as foods, pharmaceuticals, biopreservation, cosmetics, etc. 12 The extraction process of the present study resulted in a yield of 2.78%, which is higher than that obtained in other Algerian regions like Tizi-Ouzou (0.06%), Bordj-Meńai ̈el (2.2%), and jedioua Relizane (0.68%), as reported by Djenane et al 6,13 Compared to the literature dealing with AHEO reported the present study's findings agree with Djenane et al 6 regarding the volatile profile of ASHEO, with carvacrol, γterpinene, and p-cymene as the predominant compounds. In contrast, Chouitah et al 7 showed a different ASHEO chemotype, where cadinol, thymol, himachalene, and selinene were the major constituents of the volatile fraction.…”

“…Studying the chemical composition of essential oil extracted from the Algerian cultivar of S. hortensis L. ( Table 1 ) allows us to know its potential use in various applications such as foods, pharmaceuticals, biopreservation, cosmetics, etc. 12 The extraction process of the present study resulted in a yield of 2.78%, which is higher than that obtained in other Algerian regions like Tizi-Ouzou (0.06%), Bordj-Ménaïel (2.2%), and jedioua Relizane (0.68%), as reported by Djenane et al 6 , 13 and Chouitah et al 7 At the same time, no data are available in the literature about the extraction yield of ASHEO collected from Bou Saada, M’Sila Province in Algeria. Many factors affect the oil yield and the plant’s productivity, such as age, genotype, agronomics, climate, and the harvesting period, Djenane et al 6 …”