Gc-ms analysis and post-mortem distribution of felbamate in humans

Friel, Patrick N.; Logan, Barry K.; Formoso, Edward J.

doi:10.1016/s0896-6974(97)00063-7

Cited by 2 publications

(1 citation statement)

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“…A previously published GC–MS method [16] used liquid–liquid extraction, larger sample sizes (1 mL) and had a run time greater than 12 min. No LLOQ was published for this assay and blood was the only biological matrix utilized where the method was described.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive LC–MS/MS method for determination of felbamate in mouse plasma and tissues and human plasma

Hansen

Samber

Gustafson

2010

Journal of Chromatography B

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Felbamate (2-phenyl-1,3-propanediol dicarbamate) is a second generation antiepileptic drug used to treat seizures refractory to other antiepileptic drugs. With approximately 3500 new patients exposed annually, several important pharmacologic interaction questions remain unanswered necessitating the need for rapid and accurate methods of felbamate analysis in biological matrices. To this end, a rapid liquid chromatography tandem mass spectrometry (LC–MS/MS) method was developed for the measurement of felbamate in mouse plasma and tissues and human plasma. Plasma (100 µL) and tissues homogenates (100 µL of 100 mg/mL) were spiked with internal standard (carisoprodol) prior to protein precipitation with acetonitrile. Samples were chromatographed on a XBridge Phenyl, 2.5 µm, 4.6 mm × 50 mm column with quantitation by internal standard reference monitoring of the ion transitions m/z 239→117 for felbamate and m/z 261→176 for carisoprodol. Calibration curves were linear from 2.5 to 500 ng/mL in mouse or human plasma and 25–5000 pg/mg in tissue homogenates. Recoveries were greater than 97% for plasma and homogenates with accuracies >92% in any of the mouse matrices and >88% in human plasma. Comparable accuracies and precision were found with and without the use of the internal standard in preparation of the calibration curves and suggest that the internal standard may not be required.

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Section: Introductionmentioning

confidence: 99%