GC-MS analysis and in vitro antibacterial potential of volatile chemical constituents from leaves of Murrayakoenigii L. Spreng

Abstract

Murraya koenigii L. Spreng, commonly known as curry leaves, is usually employed for their flavoring properties in the food due to its distinct aroma. It is a source of essential oil that has been studied extensively for its therapeutic potentials. The volatile constituents were obtained from leaves of M. koenigii by hydro-distillation method. It was then evaluated for its antibacterial potential on four different strains of bacteria, i.e., Micrococcus luteus, Bacillus subtilis, Escherichia coli, and Pasteurella multocida by agar well-diffusion method. The volatile constituents were then subjected to GC-MS analysis for their chemical composition. The volatile constituents showed the appreciable antibacterial spectrum against all four strains. The GC-MS analysis of volatile constituents revealed the presence of 4 unidentified and 7 identified major components. The unidentified and identified components constitutes 0.1932% and 99.1089%, respectively. The identified compounds were -pinene (3.4155%), -caryophyllene (15.8231%), sabinene (59.7176%), -pinene (10 .934 5%), -phella ndrene (5.25 56%), -terpinene (2.7974 %) a nd myrcene (1.8632%). The findings clearly suggest that the volatile chemical constituents obtained from M. koenigii has pronounced antibacterial activity against the strains used and the components responsible for it were scrutinized.

Original article Antifungal efficacies of plant extracts against Alternaria solani (Ellis and Martin) Jones and Grout under in vitro condition

“…The Acronychia pedunculata extracts tested offered maximum RSA (Radical Scavenging Activities) of 65.94 % against E. coli. The antimicrobial activity of M. koenigii was estimated by well diffusion method; the maximum antibacterial activity was observed against Pasteurella multocida at 75% concentration (Mahendra et al, 2016;Gireesha and Raju, 2016;Aslam et al, 2017).…”

Aromatic profiling of Murraya koenigii leaves by Thermal Desorption Gas chromatography-Mass Spectroscopy (TD-GC-MS)

“…Interestingly, the accessions were clearly grouped in two quadrants supported by key variable compounds. The sesquiterpenes and few monoterpenes are a few variables that influence genotype differentiation and may be chemotaxonomic markers [ 17 , 18 ].

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Pharmacognostical, physicochemical and phytochemical evaluation of Huberantha senjiana (Annonaceae) leaf: An endemic tree of Gingee Hills Tamil Nadu India

“…The fluorescence assay is a valued, simple, and absolute technique for the determination of fluorescence compounds. Diverse compounds produce fluorescence once exposed to the UV light short and long wavelength (Akbar et al, 2014;Aslam and Afridi, 2018). The fluorescence analysis is considered to be the most important step in standardization with absolute scientific validation of crude drugs.…”