GC/MS analysis and in vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas and its main constituent carvacrol

Safaei‐Ghomi, Javad; Ebrahimabadi, Abdolrasoul H.; Djafari-Bidgoli, Zahra; Batooli, Hossein

doi:10.1016/j.foodchem.2009.01.051

Cited by 148 publications

(94 citation statements)

References 26 publications

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“…A diet supplemented with 100 mg/kg carvacrol-thymol (1 : 1) blend reduced weaning-induced oxidative stress. Both carvacrol and thymol have free radical scavenging activity (Mastelic et al, 2008;Archana et al, 2009;Safaei-Ghomi et al, 2009). In the current study, increased activity of antioxidants in the jejunum was observed in the weaned-CB group compared with the weaned group.…”

Section: Discussionmentioning

confidence: 99%

A carvacrol–thymol blend decreased intestinal oxidative stress and influenced selected microbes without changing the messenger RNA levels of tight junction proteins in jejunal mucosa of weaning piglets

Wei

Xue

Zhou

et al. 2017

Animal

113

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Recent studies indicate that intestinal oxidative stress and microbiota imbalance is involved in weaning-induced intestinal dysfunction in piglets. We have investigated the effect of feeding a carvacrol-thymol blend supplemented diet on intestinal redox status, selected microbial populations and the intestinal barrier in weaning piglets. The piglets (weaned at 21 days of age) were randomly allocated to two groups with six pens per treatment and 10 piglets per pen. At weaning day (21 days of age), six piglets were sacrificed before weaning to serve as the preweaning group. The weaned group was fed with a basal diet, while the weaned-CB group was fed with the basal diet supplemented with 100 mg/kg carvacrol-thymol (1 : 1) blend for 14 days. On day 7 post-weaning, six piglets from each group were sacrificed to determine intestinal redox status, selected microbial populations, messenger RNA (mRNA) transcript levels of proinflammatory cytokines and biomarkers of intestinal barrier function. Weaning resulted in intestinal oxidative stress, indicated by the increased concentration of reactive oxygen species and thiobarbituric acid-reactive substances present in the intestine. Weaning also reduced the population of Lactobacillus genus and increased the populations of Enterococcus genus and Escherichia coli in the jejunum, and increased mRNA levels of tumor necrosis factor α (TNF-α), interleukin 1β and interleukin 6 (IL-6). In addition, decreased mRNA levels of zonula occludens and occludin in the jejunal mucosa and increased plasma diamine oxidase concentrations indicated that weaning induced dysfunction of the intestinal barrier. On day 7 post-weaning, supplementation with the carvacrol-thymol blend restored weaning-induced intestinal oxidative stress. Compared with the weaned group, the weaned-CB group had an increased population of Lactobacillus genus but reduced populations of Enterococcus genus and E. coli in the jejunum and decreased mRNA levels of TNF-α. The results indicated that weaning induced intestinal oxidative stress and dysfunction of the intestinal barrier. Dietary supplementation with 100 mg/kg carvacrol-thymol (1 : 1) decreased the intestinal oxidative stress and influenced selected microbial populations without changing the biomarkers of intestinal barrier in weaning piglets.

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Section: Discussionmentioning

confidence: 99%

A carvacrol–thymol blend decreased intestinal oxidative stress and influenced selected microbes without changing the messenger RNA levels of tight junction proteins in jejunal mucosa of weaning piglets

Wei

Xue

Zhou

et al. 2017

Animal

113

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“…33,9 However, the total activity is not attributed only to the major compounds, because inter actions between different compounds can exist in a synergistic or antagonistic way to reduce free radicals. 36 …”

Section: Ic 50 Determinationmentioning

confidence: 99%

In vitro study of anti-glycation and radical scavenging activities of the essential oils of three plants from Morocco: Origanum compactum, Rosmarinus officinalis and Pelargonium asperum

Ramdan¹,

Raja²,

Amakran³

et al. 2015

Phcog J

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Introduction:We have carried out our study on the chemical composition; anti-glycation andradical scavenging activities of Origanum compactum, Rosmarinus officinalis and Pelargonium asperum essential oils (EO) that were harvested in the northeast of Morocco. These plants have been traditionally used in medicine as a chief ingredient of many polyherbal formulations for the treatment of several pathologies. Method: The phytochemical study was revealed by GC-MS. The protein glycation inhibitory activity of EO extracted from these plant tissues was evaluated in vitro using the model system of bovine serum albumin and methylglyoxal. The measure of DPPH • radical reducing power was used to evaluate the antiradical activity. To test each fraction, we used the IC50 value previously obtained for the crude oils. Results: The phytochemical study of these essential oils showed that p-Thymol, Eucalyptol and Citronellal were respectively the major components in the three investigated EO of O.compactum, R. officinal is and P. asperum. More than 90% of the total components were detected. The extracts and fractions with glycation inhibitory activity also showed antiradical activity when the DPPH • radical reducing power was measured. Conclusion: The glycation inhibitory activity was correlated with the antiradical potency of the extracts. Thus, the positive glycation inhibitory and antiradical activities of these plants might suggest a possible role in targeting aging and diabetic complications.The presence of various bioactive compounds confirms the application of these plants for various diseases by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.

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“…carvacrol or thymol. Various techniques were used: 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging assay [63,69,70,71,72], β-carotene bleaching test [68,70,72], thiobarbituric acid reactive species (TBARS) assay [67,69,73], ferric ion reducing antioxidant power (FRAP) assay [72], oxygen radical absorbance capacity assay [74] and method measuring the formation of hydroperoxydienes from linoleic acid [67]. With help of electron spin resonance (ESR) spectroscopy Fujisawa et al [75] found that eugenol and eugenol-related synthetic compounds possessed radical scavenging activity comparable with the activity of conventional antioxidants.…”

Section: Mutagenicity Anti-mutagenicity and Dna-protective Effectsmentioning

confidence: 99%

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Slameñová¹,

Horváthová²

2013

neo

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Flowers, berries, leaves, barks and roots of different plants have been used through the ages as a source of flavor in food and perfume preparations. The volatiles responsible for the flavor of botanicals can be extracted from the plant material as "essential oils" (EOs), called also volatile oils or ethereal oils. The term essential is intended to indicate that the oil is the fragrant essence of the plant from which it is extracted. EOs are constituted by hydrocarbons (monoterpenes and sesquiterpenes) and oxygenated compounds (alcohols, esters, ethers, aldehydes, ketones, lactones, phenols and phenol ethers). Of the numerous groups of naturally occurring compounds examined so far terpenes are known as fragrances and flavoring agents. The data reported in this review including the data obtained in our laboratory show that many of EOs exhibit a range of biological activities inclusive of antioxidative, anti-mutagenic and anti-carcinogenic activities. Most of them belong to phytochemicals with chemopreventive potential. On the other hand some herbal products can cause serious adverse effects. A complex research of toxic, genotoxic, anti-mutagenic and anti-carcinogenic effects of EOs is therefore very important.

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GC/MS analysis and in vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas and its main constituent carvacrol

Cited by 148 publications

References 26 publications

A carvacrol–thymol blend decreased intestinal oxidative stress and influenced selected microbes without changing the messenger RNA levels of tight junction proteins in jejunal mucosa of weaning piglets

A carvacrol–thymol blend decreased intestinal oxidative stress and influenced selected microbes without changing the messenger RNA levels of tight junction proteins in jejunal mucosa of weaning piglets

In vitro study of anti-glycation and radical scavenging activities of the essential oils of three plants from Morocco: Origanum compactum, Rosmarinus officinalis and Pelargonium asperum

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

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