GC × GC-TOFMS metabolomics analysis identifies elevated levels of plasma sugars and sugar alcohols in diabetic mellitus patients with kidney failure

Duangkumpha, Kassaporn; Jariyasopit, Narumol; Wanichthanarak, Kwanjeera; Dhakal, Esha; Wisanpitayakorn, Pattipong; Thotsiri, Sansanee; Sirivatanauksorn, Yongyut; Kitiyakara, Chagriya; Sathirapongsasuti, Nuankanya; Khoomrung, Sakda

doi:10.1016/j.jbc.2022.102445

Cited by 8 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most widespread combination is GC×GC with TOF MS. This method has several advantages over the common GC-MS, such as better metabolite coverage, separation peak capacity, sensitivity, and resolution [ 19 , 20 ]. These enhancements render GC×GC-MS a powerful tool for untargeted metabolomics, as it is capable of detecting three times the number of metabolites compared to traditional GC-MS [ 21 ].…”

Section: Methods Landscapementioning

confidence: 99%

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Demicheva,

Dordiuk,

Polanco Espino

et al. 2024

Metabolites

View full text Add to dashboard Cite

Blood metabolomics profiling using mass spectrometry has emerged as a powerful approach for investigating non-cancer diseases and understanding their underlying metabolic alterations. Blood, as a readily accessible physiological fluid, contains a diverse repertoire of metabolites derived from various physiological systems. Mass spectrometry offers a universal and precise analytical platform for the comprehensive analysis of blood metabolites, encompassing proteins, lipids, peptides, glycans, and immunoglobulins. In this comprehensive review, we present an overview of the research landscape in mass spectrometry-based blood metabolomics profiling. While the field of metabolomics research is primarily focused on cancer, this review specifically highlights studies related to non-cancer diseases, aiming to bring attention to valuable research that often remains overshadowed. Employing natural language processing methods, we processed 507 articles to provide insights into the application of metabolomic studies for specific diseases and physiological systems. The review encompasses a wide range of non-cancer diseases, with emphasis on cardiovascular disease, reproductive disease, diabetes, inflammation, and immunodeficiency states. By analyzing blood samples, researchers gain valuable insights into the metabolic perturbations associated with these diseases, potentially leading to the identification of novel biomarkers and the development of personalized therapeutic approaches. Furthermore, we provide a comprehensive overview of various mass spectrometry approaches utilized in blood metabolomics research, including GC-MS, LC-MS, and others discussing their advantages and limitations. To enhance the scope, we propose including recent review articles supporting the applicability of GC×GC-MS for metabolomics-based studies. This addition will contribute to a more exhaustive understanding of the available analytical techniques. The Integration of mass spectrometry-based blood profiling into clinical practice holds promise for improving disease diagnosis, treatment monitoring, and patient outcomes. By unraveling the complex metabolic alterations associated with non-cancer diseases, researchers and healthcare professionals can pave the way for precision medicine and personalized therapeutic interventions. Continuous advancements in mass spectrometry technology and data analysis methods will further enhance the potential of blood metabolomics profiling in non-cancer diseases, facilitating its translation from the laboratory to routine clinical application.

show abstract

Section: Methods Landscapementioning

confidence: 99%

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Demicheva,

Dordiuk,

Polanco Espino

et al. 2024

Metabolites

View full text Add to dashboard Cite

show abstract

“…GC-MS is used for monitoring monosaccharides [45,69,203] which need always derivatization (Table S2, [51,76,118,202,[205][206][207][208][209][210][211][212][213][214][215][216][217][218][219]). TFAA [118] and TMS [75] derivatization methods are simple to use and the chemicals are effective for a wide range of saccharides.…”

Section: Analysis Of Saccharides By Gcmentioning

confidence: 99%

“…However, the processes produce two products of each saccharide at least, which complicates quantitation [118,204]. Other derivatization methods used are acetylation [205,210], MSTFA-TMCS [211,212,214], TBME [212], BSTFA-TMCS [118,213,216,219], BSTFA [76,205,218], and PFBO-TMS [51] labeling. Temperatures in derivatization were from ambient to 70 • C (Table S2).…”

Section: Analysis Of Saccharides By Gcmentioning

confidence: 99%

“…The minimum incubation time in reactions was 20 min. Furthermore, comprehensive 2D-separation with GC x GC-TOF-MS for plasma saccharides and sugar alcohols derivatized with MSTFA-TMCS [211] and BSTFA derivatization for mono-and disaccharides from tea leaves [76] allowed good accuracy in identification. Mostly, saccharides were analyzed with temperature gradients from 60, 75, or 100 • C to 300 or 325 • C when they were studied as silylated compounds.…”

Section: Analysis Of Saccharides By Gcmentioning

confidence: 99%

See 1 more Smart Citation

Research of saccharides and related biocomplexes: A review with recent techniques and applications

Sirén

2024

J of Separation Science

View full text Add to dashboard Cite

Saccharides and biocompounds as saccharide (sugar) complexes have various roles and biological functions in living organisms due to modifications via nucleophilic substitution, polymerization, and complex formation reactions. Mostly, mono‐, di‐, oligo‐, and polysaccharides are stabilized to inactive glycosides, which are formed in metabolic pathways. Natural saccharides are important in food and environmental monitoring. Glycosides with various functionalities are significant in clinical and medical research. Saccharides are often studied with the chromatographic methods of hydrophilic interaction liquid chromatography and anion exchange chromatograpy, but also with capillary electrophoresis and mass spectrometry with their on‐line coupling systems. Sample preparation is important in the identification of saccharide compounds. The cases discussed here focus on bioscience, clinical, and food applications.

show abstract

“…Recently, associations between increased erythritol blood levels and metabolic disorders and/or cardiovascular diseases have been reported (Rebholz et al, 2018;Wang et al, 2019;Witkowski et al, 2023). In addition, several metabolomic profiling studies reported that elevated circulating erythritol concentrations, together with other metabolites, were observed in patients with type II (T2) diabetes and related vascular and non-vascular complications (Chen et al, 2016;Duangkumpha et al, 2022;Menni et al, 2013;Moon et al, 2023;Shao et al, 2022) and/or cardiovascular disease (Fu et al, 2022). Witkowski et al (2023) examined the association between circulating blood erythritol levels and major adverse cardiovascular events (MACE): death, nonfatal myocardial infarction or nonfatal cerebrovascular accident (stroke) in one cohort (USA) consisting of 1157 stable patients aged 52-76 years and undergoing cardiac risk assessment for symptom evaluation.…”

Section: Studies On Circulating Erythritolmentioning

confidence: 99%

Re‐evaluation of erythritol (E 968) as a food additive

Younes,

Aquilina,

Castle

et al. 2023

EFS2

View full text Add to dashboard Cite

This opinion addresses the re-evaluation of erythritol (E 968) as food additive and an application for its exemption from the laxative warning label requirement as established under Regulation (EU) No 1169/2011. Erythritol is a polyol obtained by fermentation with Moniliella pollinis BC or Moniliella megachiliensis KW3-6, followed by purifications and drying. Erythritol is readily and dose-dependently absorbed in humans and can be metabolised to erythronate to a small extent. Erythritol is then excreted unchanged in the urine. It does not raise concerns regarding genotoxicity. The dataset evaluated consisted of human interventional studies. The Panel considered that erythritol has the potential to cause diarrhoea in humans, which was considered adverse because its potential association with electrolyte and water imbalance. The lower bound of the range of no observed adverse effect levels (NOAELs) for diarrhoea of 0.5 g/kg body weight (bw) was identified as reference point. The Panel considered appropriate to set a numerical acceptable daily intake (ADI) at the level of the reference point. An ADI of 0.5 g/kg bw per day was considered by the Panel to be protective for the immediate laxative effect as well as potential chronic effects, secondary to diarrhoea. The highest mean and 95th percentile chronic exposure was in children (742 mg/kg bw per day) and adolescents (1532 mg/kg bw per day). Acute exposure was maximally 3531 mg/kg bw per meal for children at the 99th percentile. Overall, the Panel considered both dietary exposure assessments an overestimation. The Panel concluded that the exposure estimates for both acute and chronic dietary exposure to erythritol (E 968) were above the ADI, indicating that individuals with high intake may be at risk of experiencing adverse effects after single and repeated exposure. Concerning the new application, the Panel concluded that the available data do not support the proposal for exemption.

show abstract

GC × GC-TOFMS metabolomics analysis identifies elevated levels of plasma sugars and sugar alcohols in diabetic mellitus patients with kidney failure

Cited by 8 publications

References 42 publications

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Research of saccharides and related biocomplexes: A review with recent techniques and applications

Re‐evaluation of erythritol (E 968) as a food additive

Contact Info

Product

Resources

About