GC-FID determination of nucleobases guanine, adenine, cytosine, and thymine from DNA by precolumn derivatization with isobutyl chloroformate

Brohi, Rafi O Zaman; Khuhawar, Muhammad Yar; Khuhawar, Taj Muhammad Jahangir

doi:10.1186/s40543-016-0090-9

Cited by 20 publications

(8 citation statements)

References 35 publications

(31 reference statements)

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“…The performance between classical chromatography methods and our proposed electrochemical method were compared in Table S2. The proposed modified electrode had the capacity to provide satisfactory recovery results and limit of detection in comparison to classical methods in literature in only 4 min [43][44][45]. To build calibration curves, the simultaneous detection of UA and DNA nucleobases using GCE/GO-MWCNT-CHT were investigated using DPV.…”

Section: Simultaneous Electrochemical Detection Of Nucleobases In Prementioning

confidence: 99%

Simultaneous Determination of Four DNA bases at Graphene Oxide/Multi-Walled Carbon Nanotube Nanocomposite-Modified Electrode

et al. 2020

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In this study, we developed a modified glassy carbon electrode (GCE) with graphene oxide, multi-walled carbon nanotube hybrid nanocomposite in chitosan (GCE/GO-MWCNT-CHT) to achieve simultaneous detection of four nucleobases (i.e., guanine (G), adenine (A), thymine (T) and cytosine (C)) along with uric acid (UA) as an internal standard. The nanocomposite was characterized using TEM and FT-IR. The linearity ranges were up to 151.0, 78.0, 79.5, 227.5, and 162.5 µM with a detection limit of 0.15, 0.12, 0.44, 4.02, 4.0, and 3.30 µM for UA, G, A, T, and C, respectively. Compared to a bare GCE, the nanocomposite-modified GCE demonstrated a large enhancement (~36.6%) of the electrochemical active surface area. Through chronoamperometric studies, the diffusion coefficients (D), standard catalytic rate constant (Ks), and heterogenous rate constant (Kh) were calculated for the analytes. Moreover, the nanocomposite-modified electrode was used for simultaneous detection in human serum, human saliva, and artificial saliva samples with recovery values ranging from 95% to 105%.

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Section: Simultaneous Electrochemical Detection Of Nucleobases In Prementioning

confidence: 99%

Simultaneous Determination of Four DNA bases at Graphene Oxide/Multi-Walled Carbon Nanotube Nanocomposite-Modified Electrode

et al. 2020

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“…These quantified metabolites belonged to 89 various metabolic pathways (and 35 pathways with at least 3 metabolites mapped onto each of them), such as methylhistidine metabolism, thiamine metabolism, glycine and serine metabolism, glucose-alanine cycle, biotin metabolism, carnitine synthesis, transfer of acetyl groups into mitochondria, urea cycle, methionine metabolism, homocysteine degradation, alanine metabolism among others ( Supplementary Table S3 ). Recently, GC-FID combined with precolumn derivatization with isobutyl chloroformate was used for confident determination of nucleobases guanine, adenine, cytosine, and thymine from DNA samples (33). Other studies have focused on detection of the food chemicals, i.e., caffeine in coffee grains using GC-FID as well (34).…”

Section: Resultsmentioning

confidence: 99%

Comparison of a GC-Orbitrap-MS with Parallel GC-FID Capabilities for Metabolomics of Human Serum

Misra

Bassey

Olivier

2019

Preprint

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Gas chromatography mass spectrometry (GC-MS) platforms for use in high throughput and discovery metabolomics have heavily relied on time of flight (ToF), and low resolution quadrupole and ion trap mass spectrometers and are typically run in electron ionization (EI) modes for matching spectral libraries. Traditionally, detectors such as flame ionization detection (FID), have also helped in identification and quantification of compounds in complex samples for diverse clinical applications, i.e., fatty acids. We probed if combination of FID in line with a high-resolution instrument like a GC-Orbitrap-MS may confer advantages over traditional mass spectrometry using EI.We used a commercially available human serum sample to enhance the chemical space of serum using an advanced high resolution mass spectrometry (HR-MS) platform (QExactive Orbitrap-MS) with an FID feature for confident metabolite identification to assess the suitability of the platform for routine clinical metabolomics research. Using the EI mode, we quantified 294 metabolites in human serum using GC-Orbitrap-MS. These metabolites belonged to 89 biological pathways in KEGG. Following a sample split, using an in-line FID analysis, 1117 peaks were quantified. Moreover, representative peaks from FID and their corresponding MS counterparts showed a good correspondence when compared for relative abundance.Our study highlights the benefits of the use of a higher mass accuracy instrument for untargeted GC-MS-based metabolomics not only with EI mode but also orthogonal detection method such as FID, for robust and orthogonal quantification, in future studies addressing complex biological samples in clinical set ups.

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“…The potential for using the AuNPs as a biochemical sensor for the determination of thymine was evaluated by comparing the linearity range and LOD of GC, 4 LC-MS, 37 glass carbon electrode-linear sweep voltammetry (GCE-LSV), 7 silicon carbon nanoparticles modified glassy carbon electrode-differential pulse voltammetry (SiCNPs/GCE-DPV), 38 and titanium dioxide nanoparticles-magnesium doped zeolite Y modified carbon paste electrode-differential pulse voltammetry (TiO 2 NPs-MgY/ZMCPE-DPV) 39 ( Table 3 ). The present method showed a high sensitivity for the detection of thymine compared to GC, GCE-LSV and SiCNP/GCE-DPV.…”

Section: Resultsmentioning

confidence: 99%

“…Various analytical techniques like gas chromatography (GC), 4 high performance liquid chromatography (HPLC), 5 nuclear magnetic resonance (NMR) spectrometry, 6 cyclic voltammetry (CV), 7,8 uorescence, 9 the immunocytochemical method (ICM) 10 and colorimetry 11 are used for the determination of thymine in a variety of samples. GC, HPLC, NMR, CV and the ICM method are found to be expensive and tedious with laborious sample preparation steps prior to instrumental analysis.…”

Section: Introductionmentioning

confidence: 99%

Experimental and theoretical approaches for the selective detection of thymine in real samples using gold nanoparticles as a biochemical sensor

et al. 2018

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GC-FID determination of nucleobases guanine, adenine, cytosine, and thymine from DNA by precolumn derivatization with isobutyl chloroformate

Abstract: Background: Adenine (A), guanine (G), cytosine (C), thymine (T) and uracil (U) are building blocks and major constituents of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The metabolic pathway of DNA and RNA is strongly related to A, G, C, T, and U.

Cited by 20 publications

References 35 publications

Simultaneous Determination of Four DNA bases at Graphene Oxide/Multi-Walled Carbon Nanotube Nanocomposite-Modified Electrode

Simultaneous Determination of Four DNA bases at Graphene Oxide/Multi-Walled Carbon Nanotube Nanocomposite-Modified Electrode

Comparison of a GC-Orbitrap-MS with Parallel GC-FID Capabilities for Metabolomics of Human Serum

Experimental and theoretical approaches for the selective detection of thymine in real samples using gold nanoparticles as a biochemical sensor

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