GbMPK3, a mitogen-activated protein kinase from cotton, enhances drought and oxidative stress tolerance in tobacco

Long, Lu; Gao, Wei; Xu, Li; Liu, Min; Luo, Xiangyin; He, Xin; Yang, Xiyan; Zhang, Xianlong; Zhu, Longfu

doi:10.1007/s11240-013-0392-1

Cited by 43 publications

(20 citation statements)

References 48 publications

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“…Tolerance to drought stress, which is enhanced by NPK1 , may activate the oxidative signaling pathway [ 19 ]. In tobacco, GbMPK3 increases ROS scavenging under drought stress [ 74 ]. The MAPKKKs that are activated under drought stress may promote ROS scavenging to control the concentrations of ROS.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize

Zhou

Gao

et al. 2015

PLoS ONE

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The mitogen-activated protein kinase (MAPK) cascade is an evolutionarily conserved signal transduction pathway that is involved in plant development and stress responses. As the first component of this phosphorelay cascade, mitogen-activated protein kinase kinase kinases (MAPKKKs) act as adaptors linking upstream signaling steps to the core MAPK cascade to promote the appropriate cellular responses; however, the functions of MAPKKKs in maize are unclear. Here, we identified 71 MAPKKK genes, of which 14 were novel, based on a computational analysis of the maize (Zea mays L.) genome. Using an RNA-seq analysis in the leaf, stem and root of maize under well-watered and drought-stress conditions, we identified 5,866 differentially expressed genes (DEGs), including 8 MAPKKK genes responsive to drought stress. Many of the DEGs were enriched in processes such as drought stress, abiotic stimulus, oxidation-reduction, and metabolic processes. The other way round, DEGs involved in processes such as oxidation, photosynthesis, and starch, proline, ethylene, and salicylic acid metabolism were clearly co-expressed with the MAPKKK genes. Furthermore, a quantitative real-time PCR (qRT-PCR) analysis was performed to assess the relative expression levels of MAPKKKs. Correlation analysis revealed that there was a significant correlation between expression levels of two MAPKKKs and relative biomass responsive to drought in 8 inbred lines. Our results indicate that MAPKKKs may have important regulatory functions in drought tolerance in maize.

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Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize

Zhou

Gao

et al. 2015

PLoS ONE

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“…To date, an increasing number of studies have shown that MAPKs can regulate plant development, growth and responses to abiotic/biotic stress. In cotton, GhMPK2 and GbMPK3 are upregulated by diverse abiotic stresses and likely play a role in drought and oxidative stress tolerance [ 21 , 22 ]. GhMPK6 plays an important role in ABA-induced CAT1 expression and H 2 O 2 production [ 23 ], whereas GhMPK6a negatively regulates responses to osmotic stress and bacterial infection [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cotton

Zhang

Wang

et al. 2014

BMC Plant Biol

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BackgroundMitogen-activated protein kinase (MAPK) cascades play a crucial role in plant growth and development as well as biotic and abiotic stress responses. Knowledge about the MAPK gene family in cotton is limited, and systematic investigation of MAPK family proteins has not been reported.ResultsBy performing a bioinformatics homology search, we identified 28 putative MAPK genes in the Gossypium raimondii genome. These MAPK members were anchored onto 11 chromosomes in G. raimondii, with uneven distribution. Phylogenetic analysis showed that the MAPK candidates could be classified into the four known A, B, C and D groups, with more MAPKs containing the TEY phosphorylation site (18 members) than the TDY motif (10 members). Furthermore, 21 cDNA sequences of MAPKs with complete open reading frames (ORFs) were identified in G. hirsutum via PCR-based approaches, including 13 novel MAPKs and eight with homologs reported previously in tetraploid cotton. The expression patterns of 23 MAPK genes reveal their important roles in diverse functions in cotton, in both various developmental stages of vegetative and reproductive growth and in the stress response. Using a reverse genetics approach based on tobacco rattle virus-induced gene silencing (TRV-VIGS), we further verified that MPK9, MPK13 and MPK25 confer resistance to defoliating isolates of Verticillium dahliae in cotton. Silencing of MPK9, MPK13 and MPK25 can significantly enhance cotton susceptibility to this pathogen.ConclusionsThis study presents a comprehensive identification of 28 mitogen-activated protein kinase genes in G. raimondii. Their phylogenetic relationships, transcript expression patterns and responses to various stressors were verified. This study provides the first systematic analysis of MAPKs in cotton, improving our understanding of defense responses in general and laying the foundation for future crop improvement using MAPKs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0345-9) contains supplementary material, which is available to authorized users.

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“…CRISPR/Cas9 has been successfully used for gene editing in important crops and model systems such as rice, wheat, Arabidopsis, Nicotiana , Sorghum, poplar, maize, and tomato ( Li et al, 2013 ; Nekrasov et al, 2013 ; Brooks et al, 2014 ; Fauser et al, 2014 ; Liang et al, 2014 ; Shan et al, 2014 ; Xu et al, 2014 ; Zhang H. et al, 2014 ; Fan et al, 2015 ; Mao et al, 2016 ). A growing number of agronomically useful genes are being identified in cotton, mostly involved in stress resistance and fiber development, and it is becoming urgent to develop a working CRISPR/Cas9 system for this crop ( Jin and Liu, 2008 ; Taliercio et al, 2010 ; Gao et al, 2011 , 2016b ; Pan, 2013 ; Long et al, 2014 ). Recently, several reports have described the application of CRISPR/Cas9 in cotton targeting MYB25, GFP, GhVP, GhCLA1 , or GhARG for genome editing, suggesting that CRISPR/Cas9 can be effectively used for cotton genome editing ( Chen et al, 2017 ; Janga et al, 2017 ; Li C. et al, 2017 ; Wang P. et al, 2017 ; Wang Y. et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genome Editing in Cotton with the CRISPR/Cas9 System

et al. 2017

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Genome editing is an important tool for gene functional studies as well as crop improvement. The recent development of the CRISPR/Cas9 system using single guide RNA molecules (sgRNAs) to direct precise double strand breaks in the genome has the potential to revolutionize agriculture. Unfortunately, not all sgRNAs are equally efficient and it is difficult to predict their efficiency by bioinformatics. In crops such as cotton (Gossypium hirsutum L.), with labor-intensive and lengthy transformation procedures, it is essential to minimize the risk of using an ineffective sgRNA that could result in the production of transgenic plants without the desired CRISPR-induced mutations. In this study, we have developed a fast and efficient method to validate the functionality of sgRNAs in cotton using a transient expression system. We have used this method to validate target sites for three different genes GhPDS, GhCLA1, and GhEF1 and analyzed the nature of the CRISPR/Cas9-induced mutations. In our experiments, the most frequent type of mutations observed in cotton cotyledons were deletions (∼64%). We prove that the CRISPR/Cas9 system can effectively produce mutations in homeologous cotton genes, an important requisite in this allotetraploid crop. We also show that multiple gene targeting can be achieved in cotton with the simultaneous expression of several sgRNAs and have generated mutations in GhPDS and GhEF1 at two target sites. Additionally, we have used the CRISPR/Cas9 system to produce targeted gene fragment deletions in the GhPDS locus. Finally, we obtained transgenic cotton plants containing CRISPR/Cas9-induced gene editing mutations in the GhCLA1 gene. The mutation efficiency was very high, with 80.6% of the transgenic lines containing mutations in the GhCLA1 target site resulting in an intense albino phenotype due to interference with chloroplast biogenesis.

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GbMPK3, a mitogen-activated protein kinase from cotton, enhances drought and oxidative stress tolerance in tobacco

Cited by 43 publications

References 48 publications

RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize

RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize

Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cotton

Genome Editing in Cotton with the CRISPR/Cas9 System

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