GATA4 and GATA5 are essential for heart and liver development in Xenopusembryos

Haworth, Kim E.; Kotecha, Surendra; Mohun, Timothy J.; Latinkic, Branko

doi:10.1186/1471-213x-8-74

Cited by 49 publications

(54 citation statements)

References 57 publications

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“…These mouse findings seemed to be supported by initial Xenopus studies reporting only a minor role for GATA5 in heart formation (Peterkin et al, 2007). However, a recent report claims instead that Xenopus GATA5 is essential for early heart development (Haworth et al, 2008).…”

Section: Introductionmentioning

confidence: 81%

“…In contrast, when tlMOs against GATA4 and 5 are injected, the GATA4 tlMO causes a more severe phenotype than the GATA5 tlMO, which shows only minimal effects on cardiac differentiation marker gene expression (data not shown, as in Peterkin et al, 2007). The efficiency of both GATA4 and GATA5 spMOs was assessed by monitoring the status of the endogenous full-length genes by RT-PCR as previously shown (Haworth et al, 2008). Injection of GATA4 or GATA5 spMOs resulted in viable embryos and caused significant reduction in the level of the expected full-length mRNAs (Fig.…”

Section: Wnt11b Differentially Mediates Function Of Gata Factors Durimentioning

confidence: 93%

“…GATA proteins are transcription factors, which regulate diverse processes during embryogenesis and in adults (Patient and McGhee, 2002). The GATA4, 5, and 6 genes are all important in vertebrates for endoderm formation (Weber et al, 2000;Afouda et al, 2005) and are central regulators of heart development (Gove et al, 1997;Reiter et al, 1999;Peterkin et al, 2003Peterkin et al, , 2007Holtzinger and Evans, 2005;Afouda et al, 2008;Haworth et al, 2008;Takeuchi and Bruneau, 2009). They have overlapping spatial and temporal expression patterns and regulate cardiomyogenesis in a partially redundant manner (Holtzinger and Evans, 2007;Peterkin et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Peterkin et al (2007) used translation blocking morpholinos against GATA5 when they observed no significant effect on cardiogenesis, while Haworth et al (2008) designed new spliceblocking morpholinos for their investigation to suggest an important role for GATA5 in heart formation. While these observations may cast doubt on the efficiency of the original translation blocking GATA5 MO, we may also raise questions about the specificity of the newly designed splice morpholino, particularly since Haworth et al (2008) failed to reverse the effects caused by their splice-blocking GATA5 morpholino in attempted rescue experiments. It is clearly important to settle this controversy about the role of GATA5 in Xenopus cardiogenesis, not least because of the implications that solving this question in Xenopus has for the role of GATA5 in vertebrate heart development in general.…”

Section: Introductionmentioning

confidence: 99%

“…Using translation-blocking (Afouda et al, 2005;Peterkin et al, 2007) and splice junction-blocking morpholinos (Haworth et al, 2008), we uncover differences in the requirements of the GATA genes for different steps in cardiogenesis. We show that loss of GATA4 and 6 result in the inhibition of early (cardiogenic) and late (cardiomyocyte differentiation) maker gene expression, in contrast to GATA5 whose inhibition affects only differentiation markers.…”

Section: Introductionmentioning

confidence: 99%

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Different requirements for GATA factors in cardiogenesis are mediated by non‐canonical Wnt signaling

Afouda¹,

Hoppler²

2011

Developmental Dynamics

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GATA factors and Wnt signals are key regulators of vertebrate cardiogenesis, but specific roles for individual GATA factors and how they interact with Wnt signaling remain unknown. We use loss of function and overexpression approaches to elucidate how these molecules regulate early cardiogenesis in Xenopus. In order to minimize indirect effects due to abnormal early embryogenesis, we use pluripotent embryonic tissues as cardiogenic assays. We confirm central roles for GATA4, 5, and 6 in cardiogenesis, but also discover individual and different requirements. We show that GATA4 or 6 regulate both cardiogenic potential and subsequent cardiomyocyte differentiation but that GATA5 is involved in regulating cardiomyocyte differentiation. We also show that Wnt11b signaling can rescue reduced cardiac differentiation resulting from loss of function of GATA4 and 6 but not GATA5. We conclude that Wnt11b mediates the differential requirements for GATA factors during vertebrate cardiogenesis. Developmental Dynamics 240:649-662,

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Section: Introductionmentioning

confidence: 81%

Section: Wnt11b Differentially Mediates Function Of Gata Factors Durimentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Different requirements for GATA factors in cardiogenesis are mediated by non‐canonical Wnt signaling

Afouda¹,

Hoppler²

2011

Developmental Dynamics

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Xenopus: An emerging model for studying congenital heart disease

Kaltenbrun

Tandon

Amin

et al. 2011

Birth Defects Research

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Congenital heart defects affect nearly 1% of all newborns and are a significant cause of infant death. Clinical studies have identified a number of congenital heart syndromes associated with mutations in genes that are involved in the complex process of cardiogenesis. The African clawed frog, Xenopus, has been instrumental in studies of vertebrate heart development and provides a valuable tool to investigate the molecular mechanisms underlying human congenital heart diseases. In this review, we discuss the methodologies that make Xenopus an ideal model system to investigate heart development and disease. We also outline congenital heart conditions linked to cardiac genes that have been well-studied in Xenopus and describe some emerging technologies that will further aid in the study of these complex syndromes.

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Gene expression analysis of the Xenopus laevis early limb bud proximodistal axis

Hudson

Bromell

Day

et al. 2022

Developmental Dynamics

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Background Limb buds develop as bilateral outgrowths of the lateral plate mesoderm and are patterned along three axes. Current models of proximal to distal patterning of early amniote limb buds suggest that two signals, a distal organizing signal from the apical epithelial ridge (AER, Fgfs) and an opposing proximal (retinoic acid [RA]) act early on pattern this axis. Results Transcriptional analysis of stage 51 Xenopus laevis hindlimb buds sectioned along the proximal‐distal axis showed that the distal region is distinct from the rest of the limb. Expression of capn8.3, a novel calpain, was located in cells immediately flanking the AER. The Wnt antagonist Dkk1 was AER‐specific in Xenopus limbs. Two transcription factors, sall1 and zic5, were expressed in distal mesenchyme. Zic5 has no described association with limb development. We also describe expression of two proximal genes, gata5 and tnn, not previously associated with limb development. Differentially expressed genes were associated with Fgf, Wnt, and RA signaling as well as differential cell adhesion and proliferation. Conclusions We identify new candidate genes for early proximodistal limb patterning. Our analysis of RA‐regulated genes supports a role for transient RA gradients in early limb bud in proximal‐to‐distal patterning in this anamniote model organism.

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GATA4 and GATA5 are essential for heart and liver development in Xenopusembryos

Cited by 49 publications

References 57 publications

Different requirements for GATA factors in cardiogenesis are mediated by non‐canonical Wnt signaling

Different requirements for GATA factors in cardiogenesis are mediated by non‐canonical Wnt signaling

Xenopus: An emerging model for studying congenital heart disease

Gene expression analysis of the Xenopus laevis early limb bud proximodistal axis

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