Gastroprotective potential of frutalin, a d-galactose binding lectin, against ethanol-induced gastric lesions

Abstract

The present study was designed to verify whether frutalin (FTL) affords gastroprotection against the ethanol-induced gastric damage and to examine the underlying mechanism(s). Gastric damage was induced by intragastric administration of 0.2 ml of ethanol (96%). Mice in groups were pretreated with FTL (0.25, 0.5 and 1 mg/kg; i.p.), cimetidine (100 mg/kg; p.o.), or vehicle (0.9% of NaCl, 10 mL/kg; p.o.), 30 min before ethanol administration. They were sacrificed 30 min later, the stomachs excised, and the mucosal lesion area (mm²) measured by planimetry. Gastroprotection was assessed in relation to inhibition of gastric lesion area. To study the gastroprotective mechanism(s), its relations to capsaicin-sensitive fibers, endogenous prostaglandins, nitric oxide, sulphydryls, ATP-sensitive potassium channels, adrenoceptors, opioid receptors and calcium channels were analyzed. Treatments effects on ethanol-associated oxidative stress markers GSH and MDA were measured in gastric tissue. FTL afforded a dose-unrelated gastroprotection against the ethanol damage. However, it failed to prevent the ethanol-induced changes in the levels of GSH and MDA. It was observed that the gastroprotection by FTL was greatly reduced in animals pretreated with capsazepine, indomethacin, L-NAME or glibenclamide. Considering the results, it is suggested that the FTL could probably be a good therapeutic agent for the development of new medicine for the treatment of gastric ulcer.

The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/KATPSignaling Pathway

“…An ATP-dependent potassium channel (K ATP ) is associated with the regulation of blood flow, acid secretion, and muscle contractility of the gastric mucosa [ 44 ]. Some compounds, such as diazoxide, inhibit ethanol-induced gastric mucosal damage through the opening of K ATP channels, while glibenclamide, a blocker of these channels, attenuates gastric injuries [ 25 , 45 , 46 ]. Thus, to assess the contribution of K ATP channels to the gastroprotective effects of PrLP, pharmacological approaches were employed.…”

“…The activation of capsaicin-sensitive primary afferents was investigated using a TRPV1 antagonist, capsazepine [ 25 ]. The animals ( n = 8 per group) were pretreated with capsazepine (5 mg/kg, i.p.…”

Hydrogel and membrane scaffold formulations of Frutalin (breadfruit lectin) within a polysaccharide galactomannan matrix have potential for wound healing

“…In normal wound healing, reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and superoxide (O 2 -) act as cellular messengers to stimulate key processes, including cellular mobility, cytokine signaling and angiogenesis [50]. Such pathways are predicted to be stimulated by our Frutalin formulations, as in our previous pre-clinical studies we showed that antinociceptive and gastroprotective effects are mediated by nitrergic mechanisms [6,7].…”

Pharmacological targeting of gastric mucosal barrier with traditional Chinese medications for repairing gastric mucosal injury

“…Similar agglutinating effects are observed in plant-derived D-galactose lectins, such as frutalin (a lectin belongs to the JRL family derived from Artocarpus incisa L.). It provides gastric protection at a significantly low dose of 0.5 mg/kg and exhibits greater efficacy, far superior to cimetidine control group (100 mg/kg), therefore exhibiting favorable repair for mucosal wounds by their own cohesive properties ( Abdon, et al, 2012 ).…”