Gastroprotective effect of garlic in indomethacin induced gastric ulcer in rats

El-Ashmawy, Nahla E.; Khedr, Eman G.; El-Bahrawy, Hoda A.; Selim, Hend M.

doi:10.1016/j.nut.2016.01.010

Cited by 83 publications

(70 citation statements)

References 36 publications

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“…Free radicals play a critical part in pathophysiology mechanism of NSAIDs-induced peptic ulcer [19]. The present study revealed induction of gastric level of TBARs by oral treatment with indomethacin however these free radicals depleted GSH gastric content.…”

Section: Discussionsupporting

confidence: 38%

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Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Ma¹,

Salahuddin²

2017

Clin Exp Pharmacol

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Indomethacin provokes aggressive ulcerogenic adverse effects. Natural products with fewer side effects are therefore highly requested to attenuate its gastric ulcer effect. Vanillin is a natural compound widely used as flavoring agent which has antioxidant activity. Therefore, the aim of this study was to investigate its gastroprotective effect against indomethacin induced gastric injury. Rats were divided into four groups; first group served as control, group 2: Treated with indomethacin (25 mg/kg, po.), group 3: Pre-treated with ranitidine (reference drug) (50 mg/kg, po., 5 days) before indomethacin and group 4: Pretreated with vanillin (100 mg/kg, po., 5 days). Pre-treatment with vanillin reduced ulcer index, gastric juice volume, free, total acidity and histopathological changes induced by indomethacin. Although it reduced gastric oxidative stress, it elevated enzymatic antioxidant activity and gastric nitric oxide content. Moreover, it reduced gastric NFκB protein expression and activity as well as inhibition in levels of proinflammatory cytokines, Myeloperoxidase (MPO) and caspase 3 activities. It down regulated gene expression of TNF-α, Cytokine-Induced Neutrophil Chemo Attractant (CINC-2α) and caspase-9 while lacking effect on mucosal prostaglandin E2 (PGE2) level. Collectively, vanillin displayed gastroprotective effects in indomethacin induced gastric ulcer by anti-secretory action and cytoprotective effect via antioxidant and anti-inflammatory activities.

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Section: Discussionsupporting

confidence: 38%

“…Previous studies also noted vanillin's anti-inflammatory property [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Moreover it was proved that also vanillic acid regulated ulcerative colitis via inhibition NF-κB p65 activation and reduction in pro-inflammatory cytokines production; IL-1, IL-6 and TNF-α [33].…”

Section: Discussionmentioning

confidence: 93%

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Ma¹,

Salahuddin²

2017

Clin Exp Pharmacol

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“…According to the standard of Okabe ulcer index, the length and width of ulcer surface were measured with a vernier caliper, calculated ulcer index, and ulcer inhibition rate; and then a part of ulcer gastric tissue was sheared to determinate the content of hexosamine in the supernatant of tissue homogenate; furthermore, the residual gastric tissue was fixed in 4% formaldehyde to prepare pathological sections for further observation of ulcer formation and healing. [34][35][36] The abovementioned animals were obtained from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). This study was approved by the Ethical Committee of the Laboratory Animal Center, Shanghai University of Traditional Chinese Medicine.…”

Section: Establishment Of Gastric Ulcer Modelsmentioning

confidence: 99%

Formulation Optimization of Gastro-Retention Tablets of Paeonol and Efficacy in Treatment of Experimental Gastric Ulcer

Zhang

Han

et al. 2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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This study aims to develop a gastroretentive sustained-release drug delivery system of paeonol using floating properties and to investigate its therapeutic effects in rat models. The gastric retention tablets of paeonol (GRT-Ps) were prepared by a direct compression method, and the Box-Behnken design was used to optimize its formulation. The optimized formulation containing 15% NaHCO 3 and a 2 : 1 ratio of paeonol and HPMC-K4M floated within 1 min and remained afloat for more than 8 h in the simulated gastric fluid (200 mL, pH 1.2) and simultaneously showed the desired sustained drug release. Moreover, small tablets (3 mm) were prepared according to the same formulation and the process technology of big tablets (8 mm). A similar drug release behavior was observed between two kinds of tablets ( f 2 52), and then the evaluations of efficacy and retention capacity in vivo were conducted with small tablets. In vivo retention studies showed that the T max (2 h) of GRT-P in rat stomachs was significantly extended compared with the T max (0.5 h) of normal reference preparation. Compared with the model group, low and high doses of GRT-P could significantly inhibit the increase of malondialdehyde (MDA) in serum. Studies showed that the higher MDA content in inflammation tissue increases the inflammatory response. The ulcer inhibition rates of GRT-P in the highdose group were 59.0 and 64.1% in the ranitidine group. Results indicated that GRT-Ps had the potential for a sustained drug release and an enhanced gastric residence time with relatively high drug concentrations in the tissue distribution.Key words paeonol; gastric retention tablet; sustained release; gastric ulcer; efficacy Paeonol, also called peony phenol, is the active component of a traditional Chinese medicine, namely, moutan cortex (Paeonia suffruticosa ANDREWS, Ranunculaceae).1) Paeonol has been extensively studied for its anti-inflammatory, antioxidant, anti-atherosclerosis, anti-diabetic, and anti-mutagenic effects.2,3) However, paeonol has significant growth-inhibitory and apoptosis-inducing effects in gastric cancer cells in vitro and in vivo.4) Moreover, studies have shown that paeonol prevents inflammatory diseases of the alimentary canal, such as irritable gastric ulcer, with minimal side effects.5) However, studies on the ethanol-induced gastric ulcer effect of paeonol are lacking. Currently, the listed paeonol dosages are administered via ordinary oral tablets and injection. However, paeonol has poor water solubility, easy oxidation, and a short biological half-life. [6][7][8] The paeonol products are unable to prolong effective drug concentration in blood to influence the oral bioavailability of a drug. Therefore, the present study, which is based on the properties of paeonol, has been designed to develop gastric retention tablets using modern technology to achieve prolonged presence in the stomach, thereby improving treatment of gastric ulcer. This approach has a profound clinical significance in developing a novel sustained-release delivery syst...

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“…The parameters for determination of UI were color of the mucosa, hyperemia, hemorrhagic spots, and number of ulcers (0 = no lesions; 0.5 = hyperemia (red coloration); 1 = hemorrhagic spots, 2 = 1–5 small ulcers; 3 = many small ulcers, 4 = many small and large ulcers; 6 = stomach full of ulcers along with perforations) (Shay et al, ). Further, the percentage of the preventive index (%PI), which was related to UI, calculated using the formula (El‐Ashmawy, Khedr, El‐Bahrawy, & Selim, ):

T h e p r e v e n t i v e i n d e x = \frac{()({italicUI}_{u l c e r a t e d g r o u p} - {italicUI}_{t r e a t e d g r o u p})}{{UI}_{normalu normall normalc normale normalr normala normalt normale normald normalg normalr normalo normalu normalp}} \times 100

…”

Section: Methodsmentioning

confidence: 99%

Protective effects of aqueous extracts of some honeys against HCl/ethanol‐induced gastric ulceration in rats

et al. 2019

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This study focused on the concept of the possible protective effect of some honey types against HCl/ethanol‐induced gastric ulcers in male Wistar albino rats. Rats were pretreated with rhododendron, chestnut, and oak honey orally with doses of 1.25 and 2.5 g/kg, bw (body weight) for three consecutive days. On fourth day, nothing was applied, and after the administration of anesthesia on the fifth day, their stomachs were surgically removed to investigate the histopathological examinations. Besides analyses of some blood serum profiles and antioxidant parameters of gastric tissue, some biochemical properties of honeys were investigated to support the histopathological results. The degrees of ulcer lesions in all groups revealed a statistically significant difference (p = .011). Although this difference originated from the additional ulcerative inducing effect of some honeys, the lower concentration rhododendron honey indicated more promising data than the positive control group (pantoprazole) in consequence of the microscopic and macroscopic evaluations. Practical applications As being a member of natural products, honey has acquired fame among the studies in recent years due to its versatility as a source of food and complementary medicine. For contributing to this argument, this comprehensive study was performed and results were focused on the lower concentration of rhododendron honey thanks to its clinical potential with protecting the gastric mucosa. According to the obtained results, our suggestion came into prominence that this honey might be protecting the mucosa, better than the different concentrations of chestnut and oak honeys, by being better‐absorbed through the gastric mucosa.

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Gastroprotective effect of garlic in indomethacin induced gastric ulcer in rats

Cited by 83 publications

References 36 publications

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Gastroprotective Effect of Vanillin on Indomethacin-Induced Gastric Ulcer in Rats: Protective Pathways and Anti-Secretory Mechanism

Formulation Optimization of Gastro-Retention Tablets of Paeonol and Efficacy in Treatment of Experimental Gastric Ulcer

Protective effects of aqueous extracts of some honeys against HCl/ethanol‐induced gastric ulceration in rats

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