Gastrointestinal stromal tumors: a clinicopathological and immunohistochemical study of 65 cases

Tepeoğlu, Merıh; Özgün, Gonca; Tunca, M Zeyneb; Tezcaner, Tugan; Ozdemır, B. Handan

doi:10.5146/tjpath.2018.01427

Cited by 4 publications

(4 citation statements)

References 19 publications

(45 reference statements)

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“…These data may be explained by the high proliferative rate of this tumour . This is also confirmed by the elevated expression of Ki‐67 protein, a widely used proliferation marker, in our COM cases as compared to other canine tumours such as mast cell tumours, indolent lymphoma, gastrointestinal stromal tumours, mammary tumours, peripheral nerve sheath tumours and gliomas …”

Section: Discussionsupporting

confidence: 83%

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Zamboni

Brocca

Ferraresso

et al. 2019

Vet Comparative Oncology

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Canine oral melanoma (COM) is the most frequent tumour with oral localization in dogs. Copy number gains and amplifications of CCND1, a gene coding for Cyclin D1, are the most frequent chromosomal aberrations described in human non-UV induced melanomas. Twenty-eight cases of COM were retrieved from paraffin-blocks archives. A total of 4 μm thick sections were immunostained with an antibody against human Cyclin D1 and Ki-67. Cyclin D1 and Ki-67 expressions were scored through two counting methods. DNA was extracted from 20 μm thick sections of formalin-fixed paraffin-embedded blocks. Pathological and surrounding healthy tissue was extracted independently. Cyclin D1 immunolabelling was detected in 69% (18/26) while Ki-67 was present in 88.5% (23/26) of cases. Statistical analysis revealed correlation between two counting methods for Cyclin D1 (r = 0.54; P = .004) and Ki-67 (r = 0.56; P = .003). The correlation found between Ki-67 and Cyclin D1 indexes in 16/26 cases labelled by both antibodies (r = 0.7947; P = .0002) suggests a possible use of Cyclin D1 index as prognostic marker. Polymerase chain reaction analysis on CCND1 coding sequence revealed the presence of nine somatic mutations in seven samples producing synonymous, missense and stop codons. Since none of the single-nucleotide polymorphisms was found to be recurrent, it is suggested that overexpression of Cyclin D1 may be the consequence of alterations of CCND1 upstream regions or other genetic aberrations not detectable with the methodology used in this study. Future studies are needed to verify the potential use of Cyclin D1 index as prognostic indicator and to highlight the molecular events responsible for Cyclin D1 overexpression in COMs. K E Y W O R D S CCND1, Cyclin D1, dog, immunohistochemistry, melanoma

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Section: Discussionsupporting

confidence: 83%

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Zamboni

Brocca

Ferraresso

et al. 2019

Vet Comparative Oncology

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“…1). A total of 20 studies that fulfilled the inclusion criteria were finally included in the analysis (15,(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40). The NOS was used to verify the quality of the evidence.…”

Section: Resultsmentioning

confidence: 99%

Ki67 for evaluating the prognosis of gastrointestinal stromal tumors: A systematic review and meta‑analysis

Li¹,

Wang²,

Chen³

et al. 2022

Oncol Lett

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Overexpression of Ki67 is observed in tumor cells, and it has been suggested to be a marker for cancer prognosis. However, the relationship between Ki67 expression and the risk of recurrence of gastrointestinal stromal tumors (GISTs) remains poorly defined. In the present study, a meta-analysis was used to examine the associations between Ki67 levels and GIST recurrence. Studies reporting GIST and Ki67 were found by searching Cochrane Library, PubMed and Embase until October 14, 2021. The Newcastle-Ottawa Scale (NOS) was used to verify the quality of the evidence. Totally, 1682 patient cases were included. The odds ratio (OR) estimates and 95% confidence interval (CI) for each publication were determined by a fixed-effects (Mantel-Haenszel) model. A total of 20 studies that fulfilled the inclusion criteria were finally included in the analysis. The average score of quality evaluation was 6.4 points according to NOS. It was found that Ki67 levels were significantly higher in the NIH L group compared with the NIH VL group (OR: 0.51; 95% CI: 0.26-0.99; P= 0.04; P heterogeneity= 0.44). There was also greater Ki67 overexpression in the NIH I group compared with the NIH L group (OR: 0.45, 95% CI: 0.31-0.65; P<0.0001; P heterogeneity= 0.32), while Ki67 levels were greater in the NIH H group than in the NIH I group (OR: 0.20; 95% CI: 0.15-0.28; P<0.00001; P heterogeneity= 0.56). In conclusion, Ki67 overexpression may be a useful marker of the risk of recurrent GIST transformation.

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“…Immunohistochemically, 90.8% of GIST cases are CD117 positive, whereas CD34+ (73.3%), smooth muscle actin+ (61.7%), desmin+ (11.7%) and S100+ (28.3%). All CD117-negative cases express DOG-1 [ 19 ]. Our case was only DOG-1 positive and helped to clinch the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Extensive Degenerative Change Masquerading Histomorphology in a Giant Cystic Gastrointestinal Stromal Tumor With Rare PDGFRA Mutation

Rahman

Rahmanuddin

Sham

et al. 2020

Cureus

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Gastrointestinal stromal tumors (GISTs) are the most frequent type of mesenchymal tumors of the gastrointestinal (GI) tract, and most of the time they acquire the mutation of special kinds of genes. GISTs may be familial or inherited and affect several family members of the family or can be sporadic. The risk of GIST is increased in people with mutations in the receptor tyrosine kinase (KIT) and platelet-derived growth factor receptor alpha (PDGFRA) genes. In this report, we present a case of a large GIST with extensive cystic and degenerative change in a 76-year-old female patient with a rare Asp842-His845 deletion mutation detected in PDGFRA exon 18, that required subtotal gastrectomy with en bloc resection.

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Gastrointestinal stromal tumors: a clinicopathological and immunohistochemical study of 65 cases

Cited by 4 publications

References 19 publications

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Ki67 for evaluating the prognosis of gastrointestinal stromal tumors: A systematic review and meta‑analysis

Extensive Degenerative Change Masquerading Histomorphology in a Giant Cystic Gastrointestinal Stromal Tumor With Rare PDGFRA Mutation

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