Gastrointestinal absorption and metabolism of apple polyphenols ex vivo by the pig intestinal mucosa in the Ussing chamber

Deußer, Hannah; Rogoll, Dorothee; Scheppach, Wolfgang; Volk, Antje; Melcher, Ralph; Richling, Elke

doi:10.1002/biot.201200303

Cited by 25 publications

(19 citation statements)

References 38 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the subgroups of CGA, the following qualitative SAR was determined: CQA (≈ 1%) < CA (≈ 1.5%) < FQA (≈ 2%) < QA (≈ 4%). This is in accordance with the existing literature for CQA and CA absorption in other parts of the GIT . The abovementioned order can be explained in part by the different physicochemical properties of the subgroups (Fig.…”

Section: Discussionsupporting

confidence: 91%

“…The position of esterification of the QA moiety did not affect the absorption in the pig jejunum at the investigated concentration of 20 lM. Previous intestinal absorption models have considered other parts of the GIT and used different CGA concentrations, preventing an appropriate comparison of results [20,37]. Nevertheless, the results of Farrell et al [19] hinted at a higher absorption of 3,5-diCQA than other diCQAs.…”

Section: Discussionmentioning

confidence: 85%

“…As a consequence of the low dose of CGA used in the SAR experiments of this study (20 μM), metabolites were not detected (Table ). At higher concentrations (≥100 μM), using pig ileal mucosa, conjugation reactions of FA have been detected . Two recent in vivo studies found that a significant proportion of CGA metabolism occurs in the human upper GIT, except for 5‐CQA with metabolites including free and sulfated caffeic and FAs and others.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Structure– and dose–absorption relationships of coffee polyphenols

Erk

Hauser

Williamson³

et al. 2013

BioFactors

Self Cite

View full text Add to dashboard Cite

Chlorogenic acids (CGAs) from coffee have biological effects related to human health. Thus, specific data on their bioavailability in the upper gastrointestinal tract are of high interest, since some molecules are absorbed here and so are not metabolized by colonic microflora. Up to now, no data on structure-absorption relationships for CGAs have been published, despite this being the most consumed group of polyphenols in the western diet. To address this gap, we performed ex vivo absorption experiments with pig jejunal mucosa using the Ussing chamber model (a model simulating the mucosa and its luminal/apical side). The main coffee polyphenols, caffeoylquinic acid (CQA), feruloylquinic acid (FQA), caffeic acid (CA), dicaffeoylquinic acid (diCQA), and D-(-)-quinic acid (QA), were incubated in individual experiments equivalent to gut lumen physiologically achievable concentrations (0.2-3.5 mM). Identification and quantification were performed with HPLC-diode array detection and HPLC-MS/MS. Additionally, the presence of ABC-efflux transporters was determined by Western blot analysis. The percentages of initially applied CGAs that were absorbed through the jejunal pig mucosa were, in increasing order: diCQA, trace; CQA, ≈ 1%; CA, ≈ 1.5%; FQA, ≈ 2%; and QA, ≈ 4%. No differences were observed within the CGA subgroups. Dose-absorption experiments with 5-CQA suggested a passive diffusion (nonsaturable absorption and a linear dose-flux relationship) and its secretion was affected by NaN3 , indicating an active efflux. The ABC-efflux transporters MDR 1 and MRP 2 were identified in pig jejunal mucosa for the first time. We conclude that active efflux plays a significant role in CGA bioavailability and, further, that the mechanism of CGA absorption in the jejunum is governed by their physicochemical properties.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Structure– and dose–absorption relationships of coffee polyphenols

Erk

Hauser

Williamson³

et al. 2013

BioFactors

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is a model that simulates the mucosa and its luminal/apical side (Bergmann and others ; Clarke ). For example, Deusser and others () have used the Ussing chamber to evaluate apple polyphenol transport and their effect on mucosal integrity.…”

Section: Setting Up the Modelmentioning

confidence: 99%

In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility

Alminger

Aura

Bohn³

et al. 2014

Comp Rev Food Sci Food Safe

292

224

View full text Add to dashboard Cite

There is an increased interest in secondary plant metabolites, such as polyphenols and carotenoids, due to their proposed health benefits. Much attention has focused on their bioavailability, a prerequisite for further physiological functions. As human studies are time consuming, costly, and restricted by ethical concerns, in vitro models for investigating the effects of digestion on these compounds have been developed and employed to predict their release from the food matrix, bioaccessibility, and assess changes in their profiles prior to absorption. Most typically, models simulate digestion in the oral cavity, the stomach, the small intestine, and, occasionally, the large intestine. A plethora of models have been reported, the choice mostly driven by the type of phytochemical studied, whether the purpose is screening or studying under close physiological conditions, and the availability of the model systems. Unfortunately, the diversity of model conditions has hampered the ability to compare results across different studies. For example, there is substantial variability in the time of digestion, concentrations of salts, enzymes, and bile acids used, pH, the inclusion of various digestion stages; and whether chosen conditions are static (with fixed concentrations of enzymes, bile salts, digesta, and so on) or dynamic (varying concentrations of these constituents). This review presents an overview of models that have been employed to study the digestion of both lipophilic and hydrophilic phytochemicals, comparing digestive conditions in vitro and in vivo and, finally, suggests a set of parameters for static models that resemble physiological conditions.

show abstract

“…Samples were stored at −80 °C until analysis. LC–MS/MS system was used to analyze the amount of drug transported (Deusser et al ., ).…”

Section: Matericals and Methodsmentioning

confidence: 97%

LC–MS/MS determination and interaction of the main components from the traditional Chinese drug pair Danshen–Sanqi based on rat intestinal absorption

Huang

Zhang

Bai

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

The Chinese drug pair Danshen (Salvia miltiorrhiza)-Sanqi (Panax ginseng) has been widely used for centuries treating various cardiovascular disorders, among which salvianlic acid B (SAB), ginsenoside Rg (GRg ), ginsenoside Rb (GRb ) and notoginsenoside R (NGR ) were identified as the major components. The present study focused on the interaction between these components based on investigating their intestinal absorption using the Ussing chamber technique. The concentrations of SAB, GRg , GRb1 and NGR in the intestinal perfusate were determined by LC-MS/MS method, followed by Q (accumulative quantity) and P (apparent permeability). The results showed that all these four main components displayed very low permeabilities, which implied their poor absorption in the rat intestine. The intestinal absorption level of SAB displayed regioselectivity: duodenum < jejunum < ileum. However, there was no significant difference in the absorption of GRg and GRb in the different segments. The Q and P values of the four main components were obviously increased in jejunum when co-administrating Danshen extract with Sanqi extract. In conclusion, compatibility of Danshen and Sanqi could remarkably improve the intestinal absorption level of the main components in the pair. To some extent, this might explain the nature of the compatibility mechanisms of composite formulae in TCMs.

show abstract

Gastrointestinal absorption and metabolism of apple polyphenols ex vivo by the pig intestinal mucosa in the Ussing chamber

Cited by 25 publications

References 38 publications

Structure– and dose–absorption relationships of coffee polyphenols

Structure– and dose–absorption relationships of coffee polyphenols

In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility

LC–MS/MS determination and interaction of the main components from the traditional Chinese drug pair Danshen–Sanqi based on rat intestinal absorption

Contact Info

Product

Resources

About