Gastroesophageal transmural potential difference measured by a new constant infusion method

Hernandez, Naomi A.; Beck, Ivan T.

doi:10.1007/bf02235884

Cited by 14 publications

(3 citation statements)

References 10 publications

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“…There are numerous methodological problems with obtaining accurate readings of transmural PD along the gastrointestinal tract in vivo. These have been the subject of several publications (Hernandez & Beck 1969;Way & Diamond, 1969; Barry & Diamond, 1970;Grantham, Code & Schlegel, 1970), but no single accepted method has yet been established. The present work describes results obtained on the human intestine in vivo using a method for recording potential difference which avoids many of the disadvantages noted previously.…”

Section: Introductionmentioning

confidence: 99%

Human intestinal potential difference: recording method and biophysical implications.

Gustke¹,

McCormick²,

Ruppin³

et al. 1981

The Journal of Physiology

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SUMMARY1. The transmural electrical potential difference (PD) of the intact human small intestine was recorded with close attention to electrical symmetry, shielding from electromagnetic waves and correction for junction potentials.2. The PD.is -12 mV (mucosa-negative) in the fasting jejunum and ileum and does not change during perfusion with isotonic NaCl.3. Absorption of Na and Cl appears to be non-electrogenic and the 'resting' PD is probably generated by active anion secretion of fasting intestinal contents.4. Diffusion potentials during isotonic D-mannitol perfusion indicated higher cation selectivity in the ileum than in the jejunum.5. The calculated contribution of a free-solution path to total paracellular permeability is 55 % in the jejunum but only 15 % in the ileum. 6. No 'streaming' potential was detected during osmotic water flow, suggesting that the cation-selectivity ofthe channels is temporarily inactivated during dilatation of the lateral intercellular space.

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Section: Introductionmentioning

confidence: 99%

Human intestinal potential difference: recording method and biophysical implications.

Gustke¹,

McCormick²,

Ruppin³

et al. 1981

The Journal of Physiology

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“…The measurement of buccal [19. 20], esophageal [21,22], small intestinal [24], colonic [25] and rectal [26] PD was indeed described as both a research and diagnostic tool.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Measurement and Recording of Gastric Potential Difference and Intragastric pH in Man

Scarpignato

Galmiche

1990

Dig Dis

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Gastric potential difference (GPD) is used increasingly as an index of mucosal integrity. The technique for GPD measurement currently employed is however laborious due to the preparation and check of KCl-agarose bridges prior to each experiment. In addition, calculations – usually handmade – are time-consuming and inaccurate. Therefore, a new apparatus allowing continuous recording of basal and drug-induced changes in GPD values was developed. A commercially available 24-hour pH monitor (Proxima Light®^, Synectics Medical) was modified in order to allow GPD measurement and recording with subsequent elaboration of data. Furthermore, simultaneous measurement of GPD and intragastric pH through the use of a single stomach probe was made possible. The new method was checked towards the classical PD measuring system employing KCl-agarose bridges. The readings obtained with both systems, either in basal conditions or after aspirin, correlated in a highly significant way. Readings proved to be quite stable with a variation, for 150 consecutive values, of less than 2 mV. With this system the effect of pH modifications, obtained through H₂ receptor stimulation and inhibition, on the behaviour of GPD values was investigated in healthy volunteers. Analysis of paired data obtained from these experiments showed an inverse relationship between these two parameters. This suggests that GPD and pH should be measured together, especially during experiments aiming to study drug effects on gastric mucosa. Although the apparatus described was developed for PD measurement and recording in the stomach, it can be used easily for PD measurement across the mucosa of the entire digestive tract, for example in the esophagus, colon or rectum.

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“…In order to avoid the use of saturated KCI, that is irritant for the gut mucosa and, therefore, may induce abnormal motor activity, we used the method de scribed by Hernandez and Beck (8).…”

mentioning

confidence: 99%

Prolonged Manometric Study of the Gastroduodenal Junction in Man

Pandolfo¹,

Bortolotti²,

Nebiacolombo³

et al. 1979

Digestion

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A study of the motor activity of the gastroduodenal (GD) junction has been carried out on 8 subjects by using an original probe provided with a suction cup, that allows not only a duodenogastric (DG) pull-through, but also a prolonged recording of the pyloric, antral and duodenal motor activity, in basal condition and after intraduodenal instillation of HCl 0.1 N and intravenous infusion of cerulein. The position of the probe was controlled with fluoroscopy and transmural potential difference. The DG pull-through did not show a zone of high pressure at the GD junction, but the prolonged study showed, during HCl and cerulein administrations, a significant increase of the pyloric tonus.

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Gastroesophageal transmural potential difference measured by a new constant infusion method

Cited by 14 publications

References 10 publications

Human intestinal potential difference: recording method and biophysical implications.

Human intestinal potential difference: recording method and biophysical implications.

Simultaneous Measurement and Recording of Gastric Potential Difference and Intragastric pH in Man

Prolonged Manometric Study of the Gastroduodenal Junction in Man

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