Gastrodin protects against LPS-induced acute lung injury by activating Nrf2 signaling pathway

Zhang, Zhuo; Zhou, Jie; Song, Daqiang; Sun, Yuhong; Liao, Changli; Jiang, Xian

doi:10.18632/oncotarget.16740

Cited by 30 publications

(23 citation statements)

References 45 publications

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“…25 gastrodin may even attenuate LPS-induced acute lung injury. 10 Thus, it seems rationality that gastrodin could also protect MRC-5 cells against…”

Section: Discussionmentioning

confidence: 99%

Retracted: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC‐5 cells by up‐regulation of miR‐103

Qiao

Wang

et al. 2019

J Cellular Molecular Medi

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The beneficial function of gastrodin towards many inflammatory diseases has been identified. This study designed to see the influence of gastrodin in a cell model of chronic obstructive pulmonary disease (COPD). MRC‐5 cells were treated by LPS, before which gastrodin was administrated. The effects of gastrodin were evaluated by conducting CCK‐8, FITC‐PI double staining, Western blot, qRT‐PCR and ELISA. Besides this, the downstream effector and signalling were studied to decode how gastrodin exerted its function. And dual‐luciferase assay was used to detect the targeting link between miR‐103 and lipoprotein receptor‐related protein 1 (LRP1). LPS induced apoptosis and the release of MCP‐1, IL‐6 and TNF‐α in MRC‐5 cells. Pre‐treating MRC‐5 cells with gastrodin attenuated LPS‐induced cell damage. Meanwhile, p38/JNK and NF‐κB pathways induced by LPS were repressed by gastrodin. miR‐103 expression was elevated by gastrodin. Further, the protective functions of gastrodin were attenuated by miR‐103 silencing. And LRP1 was a target of miR‐103 and negatively regulated by miR‐103. The in vitro data illustrated the protective function of gastrodin in LPS‐injured MRC‐5 cells. Gastrodin exerted its function possibly by up‐regulating miR‐103 and modulating p38/JNK and NF‐κB pathways.

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“…25 gastrodin may even attenuate LPS-induced acute lung injury. 10 Thus, it seems rationality that gastrodin could also protect MRC-5 cells against…”

Section: Discussionmentioning

confidence: 99%

Retracted: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC‐5 cells by up‐regulation of miR‐103

Qiao

Wang

et al. 2019

J Cellular Molecular Medi

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“…4-HBA and β-gastrodin are the main phenolic constituents of GE [16]. They reduce oxidative stress by upregulating Nrf2 [17,18]. β-Gastrodin downregulated IL-6 and MAPKs [6].…”

Section: Effect Of Ege's Active Components On Mmp-1 Mmp-3 and Procomentioning

confidence: 99%

Evaluation of protective effect of cyclodextrin glucanotransferase-treated <i>Gastrodia elata</i> Blume extract on ultraviolet B-induced premature skin aging

Hwang¹,

Ngo²,

Yang³

et al. 2019

Trop. J. Pharm Res

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Purpose: To investigate the protective effect of Gastrodia elata Blume (G. elata, GE) and cyclodextrin glucanotransferase (CGTase) enzyme-treated G. elata extract (EGE) against premature skin aging using ultraviolet B (UVB)-exposed normal human dermal fibroblasts (NHDFs). Methods: The extract was characterized by liquid chromatography with tandem mass spectrometry (LC-MS/MS), ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS) and nuclear magnetic resonance spectroscopy (NMR). The expression of matrix metalloproteinases (MMP-1,3), interleukin-6 (IL-6), transforming growth factor (TGF-β1) and procollagen type I was assayed using ELISA kits. Safety evaluation of EGE's dietary administration and topical application was performed by in vivo acute oral toxicity and local lymph node tests. Results: Lower MMP-1 and IL-6 and higher procollagen type I and TGF-β1 levels were observed after treatment with EGE than with GE, indicating that EGE was more effective than GE in treating UVBinduced photoaging. With respect to phenolic composition, EGE had lower 4-hydroxybenzaldehyde (4-HBA) level and higher α-gastrodin level than GE. In UVB-irradiated NHDFs, α-gastrodin exhibited higher anti-aging activity than 4-HBA and β-gastrodin based on the expression of MMP-1, MMP-3, and procollagen type I. The in vivo data indicate that EGE was safe at concentrations of up to 2000 mg/kg for dietary administration and 0.1 % for topical application. Conclusion: EGE protects UVB-induced photoaged human skin better than GE owing to its higher αgastrodin content. Thus, EGE may be potentially useful agent in anti-aging cosmetic products.

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“…Gastrodin, a major active ingredient of Chinese herbal medicine called Gastrodia elata Blume, has been reported to exert anti-inflammatory and anti-apoptotic effects in various diseases (10,11). Importantly, Peng et al (12) found gastrodin treatment reduced reactive oxygen species production in macrophages and protected macrophages against oxidative stress-induced apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway

et al. 2018

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Diabetic retinopathy (DR), one of the most common complications of late‑phase diabetes, is associated with the ectopic apoptosis of microvascular cells. Gastrodin, a phenolic glucoside derived from Gastrodia elata Blume, has been reported to have antioxidant and anti‑inflammation activities. The aim of the present study was to investigate the effects of gastrodin on high glucose (HG)‑induced human retinal endothelial cell (HREC) injury and its underlying mechanism. The results demonstrated that HG induced cell apoptosis in HRECs, which was accompanied by increased levels of reactive oxygen species production. Gastrodin treatment significantly alleviated HG‑induced apoptosis and oxidative stress. Furthermore, HG stimulation decreased the levels of SIRT1, which was accompanied by an increase in Toll‑like receptor 4 (TLR4) expression and the levels of phosphorylated nuclear factor (NF)‑κBp65. However, the administration of gastrodin significantly inhibited the activation of the sirtuin 1 (SIRT1)/TLR4/NF‑κBp65 signaling pathway in HRECs exposed to HG. Collectively, the present study demonstrated that gastrodin may be effective against HG‑induced apoptosis and its action may be exerted through the regulation of the SIRT1/TLR4/NF‑κBp65 signaling pathway.

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Gastrodin protects against LPS-induced acute lung injury by activating Nrf2 signaling pathway

Cited by 30 publications

References 45 publications

Retracted: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC‐5 cells by up‐regulation of miR‐103

Retracted: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC‐5 cells by up‐regulation of miR‐103

Evaluation of protective effect of cyclodextrin glucanotransferase-treated <i>Gastrodia elata</i> Blume extract on ultraviolet B-induced premature skin aging

Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway

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