Gastrodin Protects against Ethanol-Induced Liver Injury and Apoptosis in HepG2 Cells and Animal Models of Alcoholic Liver Disease

Abstract

This study aims to investigate the protective effects of gastrodin (GSTD), a natural compound isolated from the root of Gastrodia elata BL., on ethanol-induced liver injury and apoptosis in HepG2 cells and animal models. For in vitro studies, GSTD was used to pre-treat the cells for 4 h followed by 600 mM of ethanol co-administration for 24 h. Alcoholic liver disease (ALD) of Sprague-Dawley (SD) rats was induced by chronic ethanol-feeding plus a single dose (5 g/kg) of acute ethanol administration, GSTD at different doses were co-administered for 8 weeks. For acute liver injury experiment of ICR mice, GSTD (100 mg/kg/d) was pre-treated for 3 d followed by ethanol administration (5 g/kg) for 3 times. The results showed that GSTD protects HepG2 cells from ethanol-induced toxicity, injury, and apoptosis significantly. Co-administered with ethanol, GSTD prevented the loss of mitochondrial membrane potential, reduced the release cytochrome c from mitochondria, and inhibited the activation of caspase-3 in HepG2 cells. In SD rats induced by chronic ethanol-feeding, GSTD significantly restored liver function and ameliorated pathological changes of the liver. In rat liver, GSTD greatly suppressed the activation of caspase-3 and inhibited hepatocellular apoptosis. In ethanol-induced acute liver injury of ICR mice, GSTD reduced liver acetaldehyde and suppressed the up-regulation of alcohol dehydrogenase (ADH) and CYP2E1 significantly. Our results demonstrate that GSTD is efficacious in protecting liver cells from ethanol-induced injury and apoptosis; it may be useful for the development of novel agents for the treatment of ALD in the future.

Retracted: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC‐5 cells by up‐regulation of miR‐103

“…Thereby, gastrodin has been considered as a potential therapeutic agent for diverse diseases such as Tourette syndrome, 7 myocardial infarction, 8 alcoholic liver disease 6,9 and acute lung injury. Thereby, gastrodin has been considered as a potential therapeutic agent for diverse diseases such as Tourette syndrome, 7 myocardial infarction, 8 alcoholic liver disease 6,9 and acute lung injury.…”

The hepato-protective effect of eupatilin on an alcoholic liver disease model of rats

“…H&E staining was carried out to determine the alterations in the histological features of the liver [ 26 ]. Chronic ingestion of ethanol causes severe pathological damage in the liver, such as necrosis, inflammation, and tissue destruction [ 56 ]. Accordingly, necrosis of the hepatocytes, chromatin condensation, degeneration of the hepatocyte structure, and dilation of the sinusoid, were observed in the ALD model.…”

“…Lee HY et al necrosis, inflammation, and tissue destruction [56]. Accordingly, necrosis of the hepatocytes, chromatin condensation, degeneration of the hepatocyte structure, and dilation of the sinusoid, were observed in the ALD model.…”

Gastrodin Alleviates Cognitive Dysfunction and Depressive-Like Behaviors by Inhibiting ER Stress and NLRP3 Inflammasome Activation in db/db Mice

“…Traditionally, Gas is used on central nervous system disorders such as dementia [36], Alzheimer’s disease [37], Parkinson’s disease [38], affective disorders, and so on. New studies find that Gas can protect against alcohol-induced liver injury in mice (50, 80, 100mg/Kg) and rats (100 mg/Kg) through inhibition of oxidative stress [39,40]. Gas can also inhibit high-glucose-induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF-κBp65 signaling pathway [41].…”