Gastric secretions affected by esophageal distention in the rat

Mard, Seyyed Ali; Naseri, Mohammad Kazem Gharib; Badavi, Mohammad

doi:10.1007/s00535-008-2288-0

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…For rats, which normally do not have reflux, it has been shown that selective destruction of the gastro-esophageal sphincter and the crural sling of the distal esophagus was significantly associated with reflux (Montedonico, Diez-Pardo et al 1999). Since the vagal nerve also represents the principal motor control of the esophagus in rats (Dong et al 2001; Mard et al 2009), any vagal disruption by a direct or indirect pharmacological effect of a test article might therefore result in weakening of the gastro-esophageal sphincter and subsequent reflux. In the literature, however, such a compound-induced sphincter weakening had not yet been described, but the reason may be the difficulty of assessing such an effect.…”

Section: Dysfunction Of the Gastro-esophageal Sphinctermentioning

confidence: 99%

Gavage-Related Reflux in Rats

et al. 2010

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After oral gavage dosing of rats, reflux may occur, resulting in serious respiratory effects and mortality. Published information on gavage-related reflux is limited, as it has not yet been a focus of research. Nevertheless, it represents a recurrent challenge in daily toxicology practice of oral gavage dosing. The absence of clear guidance and criteria for the identification and management of reflux-induced effects can limit the ability to properly interpret toxicity study results. The review presented herein includes an overview of experimental data from gavage studies in rats, in which reflux was observed, and provides a comprehensive analysis of the literature on reflux in general and the different potential pathways contributing to gavage-related reflux in rats. The article aims to increase the awareness and understanding of the pathogenesis of gavage-related reflux and provides guidance on identification of potential risk factors, as well as interpretation of histological changes and their toxicological relevance. Furthermore, differentiation of reflux-induced effects from direct compound-related toxicity and from gavage errors is addressed in particular, and the importance of nasal histology is discussed.

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Section: Dysfunction Of the Gastro-esophageal Sphinctermentioning

confidence: 99%

Gavage-Related Reflux in Rats

et al. 2010

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Simultaneous assessment of gastric emptying and secretion in rats by a novel computed tomography-based method

Jordi

Verrey

Lutz

2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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Gastric emptying and gastric secretion are two major physiological functions of the stomach. The assessment of these functions in particular in small animals is challenging; no method currently available allows the simultaneous measurement of both functions, and methods used are lethal or invasive and often limited by spatial, temporal, or quantitative resolution. Here, we report the establishment and validation of a quantitative noninvasive high-throughput computed tomography-based method to measure simultaneously gastric emptying and secretion in rats in vivo. The imaging strategy enables one to visualize stomach anatomy and to quantify stomach volume and stomach contrast agent content. The method was validated by comparing the results to classical lethal methods (stomach phenol red content and stomach wet weight). Additionally, we showed that the use of a mild anesthetic does not interfere with normal gastric function, thereby enabling high-resolution temporal studies within single animals. These combined advantages were applied to reevaluate the impact of cholecystokinin (CCK), histamine, and oral glucose solutions on gastric function with high temporal resolution. CCK inhibited gastric emptying completely for 20 min, leading to the accumulation of gastric juice in the stomach. The CCK antagonist devazepide blocked this effect. Histamine stimulated both gastric secretion and delayed emptying. Oral glucose solution emptied at a fixed rate of 24-31 cal/min and stimulated gastric secretion. These results confirm previous observations and add volumetric changes as a new dimension. As computed tomography scanners become broadly available, this method is an excellent approach to measure the combined gastric functional readout and to reduce the number of animals used.

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Gastric secretions affected by esophageal distention in the rat

Abstract: These results indicate that the vagus nerves are involved in the inhibitory effect of esophageal distension on gastric secretory function.

Cited by 2 publications

References 26 publications

Gavage-Related Reflux in Rats

Gavage-Related Reflux in Rats

Simultaneous assessment of gastric emptying and secretion in rats by a novel computed tomography-based method

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