Gastric mucosal cell protection by epidermal growth factor in primary monolayer culture of guinea pig gastric mucous cells

Shimamoto, Chikao; Hirata, Ichiro; Umegaki, Eiji; Takiuchi, Hiroya; Hiraike, Yutaka; Fujiwara, Shoko; Katsu, Ken‐ichi

doi:10.1007/s00535-003-1137-4

Cited by 5 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results suggest that the stimulatory effects of α-LA act independently of endogenous PGE 2 . In this context, several gastroprotective agents, such as epidermal growth factor and geranylgeranylacetone, have been reported to increase mucin synthesis and secretion in cultured cells through prostaglandin-independent pathways (Terano et al, 1986;Shimamoto et al, 2003). To date, the prostaglandin-independent pathways for mucin metabolism remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Bovine α-Lactalbumin Stimulates Mucus Metabolism in Gastric Mucosa

Ushida¹,

Shimokawa²,

Toida³

et al. 2007

Journal of Dairy Science

View full text Add to dashboard Cite

Bovine alpha-lactalbumin (alpha-LA), a major milk protein, exerts strong gastroprotective activity against rat experimental gastric ulcers induced by ethanol or stress. To elucidate the mechanisms underlying this activity, the influence of alpha-LA on gastric mucus metabolism was investigated in vitro and in vivo. For the in vitro study, RGM1 cells (a rat gastric epithelial cell line) were selected for observation of the direct activity of alpha-LA on gastric mucosal cells and cultured in the presence of either alpha-LA or ovalbumin (OVA), a reference protein showing no gastroprotective activity. Amounts of synthesized and secreted mucin, a major component of mucus, were determined using [3H]glucosamine as a tracer, and prostaglandin E2 (PGE2) levels in the culture medium were determined by RIA. For the in vivo study, the thickness of the mucus gel layer, a protective barrier for gastric mucosa, was evaluated histochemically in rat gastric mucosa. alpha-Lactalbumin (3 mg/mL) significantly stimulated mucin synthesis and secretion in RGM1 cells and also increased PGE2 levels in the culture medium. In contrast, OVA showed no enhancing effects under identical conditions. Neither indomethacin, a cyclo-oxygenase inhibitor, nor AH23848, a prostaglandin EP4 receptor antagonist, affected alpha-LA-induced enhancement of mucin synthesis and secretion. In vivo, oral administration of alpha-LA (300 mg/kg x 3 times/d x 7 d) increased the thickness of the mucus gel layer in rats. These results indicate that alpha-LA fortifies the mucus gel layer by stimulating mucin production and secretion in gastric mucus-producing cells, and that this enhancing effect is independent of endogenous PGE2. Comparison of the efficacy of alpha-LA with OVA suggests that the activities observed in RGM1 cells are closely related to the gastroprotective effects in rat gastric ulcer models. In conclusion, alpha-LA stimulates mucus metabolism, and this action may be responsible for its gastroprotective activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Bovine α-Lactalbumin Stimulates Mucus Metabolism in Gastric Mucosa

Ushida¹,

Shimokawa²,

Toida³

et al. 2007

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…EGFR is the receptor for EGF, and EGF is secreted by the submandibular gland, salivary gland, pancreas, and duodenum 21 . It can restrain the secretion of gastric acid, protect the gastric mucosa, and promote gastric epithelial repair and regeneration 22,23 . These functions of EGF are achieved when EGF binds to its receptor, and changes in EGFR expression directly affect the function of EGF.…”

Section: Discussionmentioning

confidence: 99%

Study of the correlation between the expression of nuclear factor kappa B and proliferation regulatory proteins and chronic superficial gastritis

Ren

et al. 2022

VOJNOSANIT PREGL

View full text Add to dashboard Cite

Background/Aim. Cell proliferation and the regulation of protein expression play an important role in gastritis, but in chronic superficial gastritis (CSG), they are rarely reported. The aim of this study was to determine the relationship between the expression of nuclear factor kappa B (NF-?B) and regulatory proteins and the rat CSG. Methods. The CSG rat model was established artificially, by chemical agents and irregular diet. The expression of epidermal growth factor receptor (EGFR) and proliferating cell nuclear antigen (PCNA) in the gastric mucosa of CSG rats was measured by immunohistochemistry, while mRNA expression levels of NF-?B p65 were detected by in situ hybridization. Results. There was more obvious infiltration of inflammatory cells in the gastric mucosa of CSG rats than in that of control rats, and the inflammation score was significantly increased. The expression levels of PCNA, EGFR, and NF-?B p65 mRNA in the gastric mucosal cells of CSG model rats increased significantly. Correlation analysis showed that the inflammation score was positively correlated with the expression levels of NF-?B p65 mRNA and EGFR, while it presented no significant correlation with the expression level of PCNA. In addition, there was a significant positive correlation between NF-?B p65 mRNA and EGFR levels. Conclusion. High expression of NF-?B and EGFR plays an important role in the occurrence and progression of CSG, and it is significantly positively correlated with the degree of inflammation in the gastric mucosa. Therefore, changes in NF-?B and EGFR expression may be used as important indicators for the assessment of CSG; changes in their expression levels are helpful to assess the degree of gastric mucosal lesions and progression of CSG.

show abstract

“…В настоящее время описаны дополнительные эффекты рабепразола [67]. В экспериментальных исследованиях у животных цитопротективный (гастропротективный) эффект был отмечен только у рабепразола и отсутствовал или был существенно менее выражен у омепразола и лансопразола [68][69][70].…”

unclassified

Choice of protonic pump inhibitor with regard to multimorbidity: a focus on rabeprazol

Трухан¹,

Degovtsov²,

Мазуров³

2019

Med. sov.

View full text Add to dashboard Cite

Acid-related diseases occupy a leading place in the structure of the incidence of the gastrointestinal tract. One of the important aspects of studying them now is to consider them in combination with coronary heart disease, hypertension, diabetes, asthma and other common diseases and conditions. Proton pump inhibitors are currently the basis for the treatment of acid-related diseases. Although all PPIs are very effective, the antisecretory effects of various drugs of this class may differ in different patients, especially in the presence of comorbidities and comorbidities. The pharmacokinetics and metabolism of rabeprazole are significantly different from those of other IPPs. The clearance of rabeprazole is largely non-enzymatic and depends little on the functioning of the cytochrome P450 (CYP) 2C19 system, which determines the predictability of the effect of rabeprazole and its safety for patients taking several drugs at the same time. A distinctive effect of rabeprazole is the activation of rabeprazole in a wide pH range, the presence of a gastroprotective effect and anti-helicobacter activity.

show abstract

Gastric mucosal cell protection by epidermal growth factor in primary monolayer culture of guinea pig gastric mucous cells

Abstract: Increased mucin biosynthesis, induced by EGF independently of PGE(2), protects gastric mucous cells.

Cited by 5 publications

References 35 publications

Bovine α-Lactalbumin Stimulates Mucus Metabolism in Gastric Mucosa

Bovine α-Lactalbumin Stimulates Mucus Metabolism in Gastric Mucosa

Study of the correlation between the expression of nuclear factor kappa B and proliferation regulatory proteins and chronic superficial gastritis

Choice of protonic pump inhibitor with regard to multimorbidity: a focus on rabeprazol

Contact Info

Product

Resources

About