Gastric microbiota features associated with cancer risk factors and clinical outcomes: A pilot study in gastric cardia cancer patients from Shanxi, China

Yu, Guoqin; Hu, Nan; Wang, Lemin; Wang, Chaoyu; Han, Xiao-You; Humphry, Mike S; Ravel, Jacques; Abnet, Christian C.; Taylor, Philip R.; Goldstein, Alisa M.

doi:10.1002/ijc.30700

Cited by 33 publications

(36 citation statements)

References 29 publications

(53 reference statements)

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“…The relative abundance of Helicobacter ranked second in our GCA tumor tissues after the relative abundance of Prevotella , and this is in contrast to the previous study . We did not find any significant difference in α diversity or the relative abundance of specific taxa by GCA stage, and this is in accordance with a previous study . However, we observed a nonsignificant decrease in Helicobacter and an increase in Prevotella with a more advanced GCA tumor stage.…”

Section: Discussionsupporting

confidence: 81%

“…28 We did not find any significant difference in α diversity or the relative abundance of specific taxa by GCA stage, and this is in accordance with a previous study. 29 However, we observed a nonsignificant decrease in Helicobacter and an increase in Prevotella with a more advanced GCA tumor stage.…”

Section: Discussioncontrasting

confidence: 52%

“…28 In addition, patients with GCA with a higher tumor grade had an increased relative abundance of H. pylori and lower α diversity in comparison with patients with a lower GCA tumor grade. 29 However, further data regarding the microbial characteristics of normal and tumor tissues from patients with ESCC and GCA are needed.…”

Section: Introductionmentioning

confidence: 99%

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Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high‐risk region of China

Shao

Vogtmann

Liu

et al. 2019

Cancer

Self Cite

105

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Background Little is known about the microbiota and upper gastrointestinal tumors. Esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinoma (GCA) occur in adjacent organs, co‐occur geographically, and share many risk factors despite being of different tissue types. Methods This study characterized the microbial communities of paired tumor and nontumor samples from 67 patients with ESCC and 36 patients with GCA in Henan, China. DNA was extracted with the MoBio PowerSoil kit. The V4 region of the 16S ribosomal RNA gene was sequenced with MiniSeq and was processed with Quantitative Insights Into Microbial Ecology 1. The linear discriminant analysis effect size method was used to identify differentially abundant microbes, the Wilcoxon rank‐sum test was used to test α diversity differences, and permutational multivariate analysis of variance was used to test for differences in β diversity. Results The microbial environments of ESCC and GCA tissues were all composed primarily of Firmicutes, Bacteroidetes, and Proteobacteria. ESCC tumor tissues contained more Fusobacterium (3.2% vs 1.3%) and less Streptococcus (12.0% vs 30.2%) than nontumor tissues. GCA nontumor tissues had a greater abundance of Helicobacter (60.5% vs 11.8%), which may have been linked to the lower α diversity (58.0 vs 102.5; P = .0012) in comparison with tumor tissues. A comparison of ESCC and GCA nontumor tissues showed that the microbial composition (P = .0040) and the α diversity (87.0 vs 58.0; P = .00052) were significantly different. No significant differences were detected for α diversity within ESCC and GCA tumor tissues. Conclusions This study showed differences in the microbial compositions of paired ESCC and GCA tumor and nontumor tissues and differences by organ site. Large‐scale, prospective cohort studies are needed to confirm these findings.

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Section: Discussionsupporting

confidence: 81%

Section: Discussioncontrasting

confidence: 52%

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Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high‐risk region of China

Shao

Vogtmann

Liu

et al. 2019

Cancer

Self Cite

105

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“…Other smaller studies detailing the GC microbiome also confirmed a reduction in H. pylori abundance in GC tissue alongside a concomitant increase in other bacterial species . The Castano‐Rodriguez study did not show the reduction in α‐diversity seen in other studies but showed that H. pylori ‐positive patients had higher species richness and serological status was a significant on diversity and composition suggesting the composition of the gastric microbiome is shaped by the immune response .…”

Section: Gastric Microbiomementioning

confidence: 74%

Other Helicobacters and the gastric microbiome

Kupčinskas

Hold

2018

Helicobacter

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The current article is a review of the most important, accessible, and relevant literature published between April 2017 and March 2018 on other Helicobacters and the gastric microbiome. The first part of the review focuses on literature describing non-Helicobacter pylori-Helicobacter (NHPH) infections in humans and animals whilst the subsequent section focuses specifically on the human gastric microbiome. Novel diagnostic methods as well as new NHPHs species have been identified in recent studies. Furthermore, our knowledge about the pathogenesis of NHPH infections has been further enhanced by important fundamental studies in cell lines and animal models. Over the last year, additional insights over the prevalence and potential prevention strategies of NHPHs have also been reported. With regard to understanding the gastric microbiome, new information detailing the structure of the gastric microbiota at different stages of H. pylori infection, within different patient geographical locations, was documented. There was also a study detailing the impact of proton-pump inhibitor usage and the effect on the gastric microbiome. Newer analysis approaches including defining the active microbiome through analysis of RNA rather than DNA-based sequencing were also published allowing the first assessments of the functional capabilities of the gastric microbiome.

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“…However, few studies focus on the microbial community in the gastric cardia. Recently, it had been reported that the gastric microflora was a major risk factor in GCC patients . Bacterial infection can cause inflammation, leading to the production of reactive oxygen species (ROS) and subsequent DNA oxidative damage and genomic instability thus posing a risk for the development of cancer .…”

Section: Introductionmentioning

confidence: 99%

Microbiological evidences for gastric cardiac microflora dysbiosis inducing the progression of inflammation

Yan

Guo

Gong

et al. 2020

J of Gastro and Hepatol

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Background and Aim Nowadays, anti‐inflammation treatment is a promising approach for preventing tumorigenesis, and human microflora is closely related to inflammation. This study aimed to investigate the gastric cardiac microbiome and identify inflammation‐related microorganisms for gastric cardiac inflammation. Methods We performed 16S rRNA sequencing on a total of 11 healthy individuals and 89 individuals with different degree of gastric cardiac inflammation. Immunohistochemistry was used for verifying candidate bacteria. Phylogenetic reconstruction of unobserved states (picrust) was used for predicting the pathways involved by cardiac microflora. Results The resident phyla in normal were Proteobacteria, Firmicutes, Bacteroides, and Actinobacteria, and the dominant genus in normal were Halomonas, shewanella, and Comamonas. In the progression of gastric cardiac inflammation, the diversity of cardiac microflora did not change (P > 0.05). However, the composition structure of cardiac microflora varied between healthy and inflamed tissues (P < 0.05). Meanwhile, there were 64 species parallel increased with inflammation degree, especially Helicobacter pylori, Lactobacillus spp. Additionally, inflammation‐related species were detected (P < 0.05), including H. pylori, Acinetobacter ursingii, and Streptococcus agalactiae. Higher H. pylori colonization was positively related to the progression of cardiac inflammation (γ coefficient = 0.678, P < 0.001), and it also influenced the cardiac microbial community structure. Cardiac microflora also participated in DNA repair pathways and is affected by the relative abundance of H. pylori (P < 0.0001). Conclusions Cardiac microflora dysbiosis, especially the increasing of the relevant abundance of H. pylori, promotes the progression of cardiac inflammation.

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Gastric microbiota features associated with cancer risk factors and clinical outcomes: A pilot study in gastric cardia cancer patients from Shanxi, China

Cited by 33 publications

References 29 publications

Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high‐risk region of China

Microbial characterization of esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high‐risk region of China

Other Helicobacters and the gastric microbiome

Microbiological evidences for gastric cardiac microflora dysbiosis inducing the progression of inflammation

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