Gastric immunolocalization and plasma profiles of acyl-ghrelin in fasted and fasted-refed prepuberal gilts

Govoni, Nadia; Iasio, Rosaria De; Cocco, Cristina; Parmeggiani, Albamaria; Galeati, Giovanna; Pagotto, Uberto; Brancia, Carla; Spinaci, Marcella; Tamanini, Carlo; Pasquali, Renato; Ferri, Gian Luca; Seren, E.

doi:10.1677/joe.1.06262

Cited by 38 publications

(43 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It plays a crucial role in regulation of many biological functions like food intake, gastrointestinal motility, hormone secretion, glucose and enzymes release. In porcine stomach, ghrelin-immunoreactive cells are most abundant in oxyntic and cardiac glands while less numerous in pyloric glands (Govoni et al, 2005). On the other hand, Hayashida (2001) reported that immunoreactive cells are abundant in all gastric mucosal areas.…”

Section: Discussionmentioning

confidence: 98%

A new set of endogenous reference genes for gene expression studies of porcine stomach

Oczkowicz¹,

Różycki²,

Piórkowska³

et al. 2010

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Gene expression analysis by Real-Time PCR requires careful selection of endogenous reference genes to obtain accurate results. We evaluated usefulness of six reference genes to expression studies of porcine stomach. We selected genes which were recently described as very stable (RPL27, RPS29, RPS13, OAZ1) and two commonly used housekeeping genes (GAPDH, ACTB). Our results indicate that OAZ1, RPS29 and RPL27 are more suitable reference genes than ACTB and GAPDH in expression studies of porcine stomach. In our study the most stable genes were OAZ1 (M=0.856), RPS29 (M=0.862) and RPL27 (M=0.892), while ACTB (M=1.03) and GAPDH (M=1.005) were less stable. RPS13 (M=1.913) appeared to be highly unstable.Set of three the most stable genes has been used to compare ghrelin (GHRL) expression in two different regions (diverticulum ventriculi and fundus ventriculi) of porcine stomach. Ghrelin mRNA was highly expressed in both regions of stomach, however level of GHRL mRNA was approximately 5-fold higher in fundus ventriculi than in diverticulum ventriculi.

show abstract

Section: Discussionmentioning

confidence: 98%

A new set of endogenous reference genes for gene expression studies of porcine stomach

Oczkowicz¹,

Różycki²,

Piórkowska³

et al. 2010

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…In most mammals, ghrelin is mainly produced in the mucosa of the stomach fundus, where it is abundant in the oxyntic, pyloric and cardiac glands (Date et al 2000, Tomasetto et al 2000, Dornonville de la Cour et al 2001, Hayashida et al 2001, Rindi et al 2002, Govoni et al 2005. A recent study (Govoni et al 2005) in pigs has shown that ghrelin is most abundant in the oxyntic and cardiac glands and less numerous in the pyloric glands.…”

Section: Introductionmentioning

confidence: 99%

Ghrelin secretion is more closely aligned to energy balance than with feeding behaviour in the grower pig

Scrimgeour¹,

Gresham²,

Giles³

et al. 2008

Journal of Endocrinology

View full text Add to dashboard Cite

Secretory characteristics of the ghrelin profile for the pig are still unknown. Our objective was to clarify the mechanisms that influence ghrelin secretion during differing feeding patterns. Pigs were initially fed a commercial pelleted diet offered ad libitum and blood samples collected for 24 h at intervals of 1 h. The pigs were then entrained for 17 days to a twice daily interval feeding regimen (0900-1000 and 1600-1700 h) and blood samples were collected for 12 h (0800-2000 h). This was followed by a similar interval feeding and blood sampling regimen with the 0900-1000 h feeding period being replaced by a sham feed where pigs were shown their usual feed but none offered. During the ad libitum feeding regimen, there was no preprandial rise or postprandial fall in circulating plasma total ghrelin concentration, which remained constant throughout the sampling period. In addition, no preprandial rise or postprandial fall in ghrelin concentrations was observed when pigs were fed either twice or once daily; however, plasma ghrelin concentration rose gradually over the 12-h sampling period during the twice daily feeding regimen and increased further when pigs were fed once per day. This increase in ghrelin levels coincided with an increase in plasma GH and non-esterified fatty acid concentrations and was not associated with either plasma glucose or insulin concentrations. These results suggest that circulating total plasma ghrelin concentrations in the pig appear to be influenced by chronic changes in energy balance rather than the feeding pattern per se.

show abstract

“…In the same way, in the present work, we could not assess whether an abnormal expression of GOAT in tumor tissue may account for the low levels of acyl ghrelin detected in our patients. The recent availability of antibodies directed against acyl ghrelin and GOAT (22,23), and adapted for immunohistochemical studies makes it possible to plan further studies to investigate the metabolism of ghrelin in endocrine tumor tissues.…”

Section: Discussionmentioning

confidence: 99%

Major hyperghrelinemia in advanced well-differentiated neuroendocrine carcinomas: report of three cases

Walter

Chardon²,

Hervieu³

et al. 2009

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: We aimed to gain insight into the functional consequences of ghrelin overproduction in patients with neuroendocrine tumors and its relations with disease characteristics and evolution. Design: We retrospectively analyzed three cases of neuroendocrine carcinomas associated with very high levels of circulating ghrelin. Methods: Between February and October 2007, serum ghrelin levels were determined in all patients with well-differentiated endocrine carcinoma referred to our center (nZ72). Three patients were found to have circulating ghrelin levels O10-fold the upper limit of normal. The clinical, biochemical, and pathological characteristics of these three patients were reviewed. The ratio between circulating acyl and total ghrelin was determined, and tumor tissue expression of ghrelin was assayed by immunohistochemistry. Results: The three patients had massive hyperghrelinemia (respectively 49 028, 63 711, and 101 996 pg/ml), with !10% of acyl ghrelin. The corresponding primary tumors were located in the pancreas, rectum, and gallbladder; all were metastatic. There was no acromegaly; there was a decrease in appetite; and body mass index was low. Serum GH levels were only slightly increased and serum IGF1 levels were normal. Immunoreactive ghrelin was detected in the tumor tissue in the two cases in which tissue material was available. All three patients died before 12 months after the diagnosis of hyperghrelinemia. Conclusion: Well-differentiated neuroendocrine carcinomas of various origins may produce markedly high levels of circulating ghrelin, without evidence of clinical or functional consequences.

show abstract

Gastric immunolocalization and plasma profiles of acyl-ghrelin in fasted and fasted-refed prepuberal gilts

Cited by 38 publications

References 41 publications

A new set of endogenous reference genes for gene expression studies of porcine stomach

A new set of endogenous reference genes for gene expression studies of porcine stomach

Ghrelin secretion is more closely aligned to energy balance than with feeding behaviour in the grower pig

Major hyperghrelinemia in advanced well-differentiated neuroendocrine carcinomas: report of three cases

Contact Info

Product

Resources

About