Gastric Helicobacters in Cats

Lecoindre, P.; Chevallier, M.; Peyrol, S; Boude, M; Ferrero, Richard L.; Labigne, Agnès

doi:10.1053/jfms.2000.0063

Cited by 24 publications

(11 citation statements)

References 26 publications

(52 reference statements)

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“…Large spiral bacteria (4 to 10 μm) identified in the stomachs of dogs were initially called Gastrospirillum hominis. They were later reclassified as Helicobacter heilmannii (Lecoindre et al, 2000). The mode of transmission still unknown, although epidemiologic studies suggest close person to person contact and intra familial spread.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Helicobacter Pylori and Escherichia Coli From Stool of Human and Pet Animals

Elkassas¹,

Khaliel²,

Shawky³

2018

AJVS

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Key words:Helicobacter pylori, gastritis, Escherichia coli, PCR, Virulence genes, human, dog and catThe recent isolation of Helicobacter pylori from cats and human has potentially important public health implications as well as other bacteria associated with H.pylori in stool samples such as E.coli. A total of 202 stool samples from human, cats and dogs were examined for detection of H.pylori and E.coli. For isolation of H.pylori, 150 stool samples were cultivated on Dent , s medium (modified skirrow , s medium) containing selective Dent , s antibiotic supplement. while the remaining 52 samples were cultivated on MacConkey, Eosine methylen blue media in attempt to isolate other associated bacteria. Colonies grossly similar to H.pylori were tested for catalase, oxidase and urease test. All human samples were tested serologically by using rapid stool antigen test (SAT). PCR assay was performed on bacterial isolates and directly from stool samples by extraction of DNA from stool by boiling method. Positive cultures were subjected to serology testing and PCR. 45samples from humans gave positive results with rapid H.pylori stool antigen test (HPSA). Results revealed that H.pylori was detected in tested samples from human and cat at a rate of 52.8%, 33.3%, respectively and could not be detected in dog. Also detected serologically at percentage of 84.9% in human stool samples. H.pylori could not be detected by PCR, while the other 52 samples were found positive for Escherichia coli, isolation at a percentage of 48%. Nine serogroups of E.coli (O1, O20, O55, O86, O114, O125, and O146) were detected in 9 samples (3 samples from each species). All serotypes were positive for Iss and Fim genes. From all stool samples subjected for isolation of E.coli we obtained 15 isolates which were introduced to PCR. 14 isolates were positive for E.coli genus (phoA gene). 9 isolates were used for detection of virulence genes (Iss, Fim, and Tsh genes). 8 isolates were positive for (Iss and Fim) gene. None was positive for (Tsh) gene. We conclude that H.pylori is very difficult to culture due to the fastidious nature of the organism and its transport and culture requirements. Also H.pylori is the main species of Helicobacter present in human. While dogs and cats harbour many gastric Helicobacter like organisms (GHLOs) and E.coli isolated from human, dogs and cats share the same characters and virulence genes.

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Section: Introductionmentioning

confidence: 99%

Characterization of Helicobacter Pylori and Escherichia Coli From Stool of Human and Pet Animals

Elkassas¹,

Khaliel²,

Shawky³

2018

AJVS

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“…Helicobacter ‐like organisms are considered to cause gastric pathology in humans and domestic animals (Heilmann and Borchard, 1991; Lavelle et al., 1994). Helicobacter ‐like organisms are observed in biopsies from the stomachs of dogs, cats, pigs and other carnivores (Lavelle et al., 1994; Eaton et al., 1996; Fox and Lee, 1997; Jalava et al., 1997; Simpson et al., 1999; Lecoindre et al., 2000;Prachasilpchai et al., 2007; Robic et al., 2007), and this genus contains several dozen species from a wide range of hosts (Seymour et al., 1994; Harper et al., 2000). Five species of Helicobacters have been cultivated from canine stomachs including Helicobacter felis , Helicobacter bizzozeronii, Helicobacter salomonis , Helicobacter bilis and Flexispira rappini (Thompson et al., 1994; Eaton et al., 1996; Hanninen et al., 1996; Jalava et al., 1997; Lee et al., 1998).…”

Section: Introductionmentioning

confidence: 99%

Detection of Atypical Cultivable Canine Gastric Helicobacter Strain and its Biochemical and Morphological Characters in Naturally Infected Dogs

Asl

Jamshidi

Mohammadi

et al. 2009

Zoonoses and Public Health

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Helicobacter-like organisms are frequently found in canine stomachs, but the relationship between such organisms and gastric pathology has not been established. However, some such organisms have zoonotic importance. The aims of this study were to evaluate the morphological and biochemical characteristics of cultivable canine gastric Helicobacter-like organisms (GHLOs) in pets and stray dogs and their prevalence in these two groups of dogs. Specimens were taken by gastroscopy from 30 clinically healthy stray dogs and 30 pet dogs. Cultures were positive from biopsies of 11/30 of stray and 6/30 of pet dogs. The isolated Helicobacters were observed by light microscopy and studied by biochemical, physiological and PCR analysis. Some of the isolated GHLO's displayed atypical shapes that were similar to Helicobacter pylori or Helicobacter acinonychis in stray dogs' cytological examinations. They had 2-3 helices and were smaller than other canine GHLOs. One of these atypical Helicobacter strains was cultured. It was not possible to distinguish such strains by routine PCR and biochemical evaluations. Electron microscopy showed a smaller Helicobacter (2 microm in length) with 2 or 3 helixes. This study demonstrates that not all canine gastric Helicobacters are 5-15 microm in length, as has been previously proposed, and portrays the need for further investigation of canine GHLOs.

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“…Existem diferentes métodos para o diagnóstico de infecção pelo Helicobacter spp em seres humanos que podem ser adaptados e usados para os animais (LECOINDRE, 2000).…”

Section: Considerações Sobre Os Métodos De Diagnósticounclassified

“…Vários métodos utilizam a endoscopia gástrica como meio de obtenção de material para exame, no entanto, outros métodos não invasivos também podem ser utilizados para obter as amostras (CHEY;WONG, 2007;CALVET et al, 2010 Outras colorações específicas têm sido aplamente utilizadas como Giemsa modificado, o azul de toluidina e a carbolfucsina na identificação das bactérias (GEYER et al, 1993;HERMANNS et al, 1995;HAPPONEN, 1996;LECOINDRE, 2000;COSTA et al, 2012).…”

Section: Considerações Sobre Os Métodos De Diagnósticounclassified

“…A frequência de ocorrência de Helicobacter spp em amostras gástricas, de biópsias endoscópicas ou necropsia, de gatos varia de 41 a 100% em animais clinicamente saudáveis (GEYER et al, 1993;OTTO, 1994;PAPASOULIOTIS et al, 1997;NEIGER et al, 1998;YAMASAKI, 1998) e de 57 a 100% em animais com êmeses recorrentes (GEYER et al, 1993;HERMANNS et al, 1995;PAPASOULIOTIS, 1997;YAMASAKI, 1998;TAKEMURA, 2009 (NEIGER et al, 1998;PAPASOULIOTIS et al, 1997;LECOINDRE et al, 2000). (HAPPONEN et al, 1996;VAIRA, 2000;OWEN, 2010 (HAPPONEN et al, 1996;.…”

Section: Estudo Da Helicobacteriose Em Cães: Frequência De Ocorrênciaunclassified

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Estudo da helicobacteriose em cães e gatos: determinação da frequência de ocorrência na mucosa gástrica de animais necropsiados e comparação entre métodos de diagnóstico

Romero¹

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Gostaria de agradecer a todas as pessoas que de qualquer forma me ajudaram e motivaram para a realização deste trabalho, nomeadamente:A Profª Lilian Rose Marques de Sá, orientadora desta tese, por me ter integrado na sua equipe, pela disponibilidade e confiança que sempre demonstrou todo o apoio e ensinamentos que me transmitiu ao longo destes anos, bem como toda a paciência para me esclarecer e motivar.Ao Departamento de Patologia pelo apoio e espaço concedido para realização deste projeto, aos funcionários da instituição, aos profissionais do laboratório de histopatologia (Cláudio Arroyo e Luciano A. Bugalho), aos técnicos da necropsia (Olivia, Raimundo N. Maciel e Edson Luiz de Souza), às secretárias da patologia (Cláudia Lima, Adriana S. Margarido e Milena F. de Oliveira).À CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) pela concessão da bolsa de mestrado para minha manutenção e despesas com a própria teste durante o período.À PROAP (Programa de Apoio à Pós-Graduação) pela concessão de auxílio técnico à pesquisa que possibilitou a compra do anticorpo anti-H.pyloria realização deste trabalho. À todas as bibliotecárias da FMVZ-USP, em especial a senhora Elza Faquim.Ao aluno de iniciação científica Leandro Haroutune Hassesian Galati e a minha amiga Aline Nardi pela ajuda nas aquisições dos prontuários e tabulações de dados para este teste.À aluna Sofia pela ajuda nos procedimentos de imuno-histoquímica e confecção de lâminas.Aos amigos e colegas do Laboratório de Gastroenterologia e Patologia Ambiental pelo convívio, conversas e boas risadas: Thiago, Alex, Maria Eugenia, Dennis, Lívia. In dogs the frequency of occurrence of gastric helicobacteriose stood at 57 %, with an association between infection and the age of the animals. In cats this frequency of occurrence was 87 % for the gold standard method (HE) and no association between infection, race, gender and age of the animals was found. Immunohistochemistry showed higher Youden index and Kappa in dogs. In cats, the specific staining based on silver, Warthin Starry, demonstrated the highest Youden and Kappa indexes among the other diagnostic methods.The determination of the frequency of occurrence of Helicobacter spp varies according to the diagnostic method adopted. The choice of method depends on the financial resources available, the samples under study, the time required, the practicality of the method and its performance.

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Gastric Helicobacters in Cats

Cited by 24 publications

References 26 publications

Characterization of Helicobacter Pylori and Escherichia Coli From Stool of Human and Pet Animals

Characterization of Helicobacter Pylori and Escherichia Coli From Stool of Human and Pet Animals

Detection of Atypical Cultivable Canine Gastric Helicobacter Strain and its Biochemical and Morphological Characters in Naturally Infected Dogs

Estudo da helicobacteriose em cães e gatos: determinação da frequência de ocorrência na mucosa gástrica de animais necropsiados e comparação entre métodos de diagnóstico

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