Gastric epithelium under organotypic perfusion culture

Kloth, Sabine; Eckert, E; Klein, Stefan J.; Monzer, Jan; Wanke, Christiane; Minuth, Will W.

doi:10.1007/s11626-998-0107-9

Cited by 16 publications

(15 citation statements)

References 15 publications

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“…Because a perfusion culture method facilitates the continuous exchange of medium and the constant removal of metabolic wastes, it has been used for various cells. [14][15][16][17] Perfusion culture methods enhanced the viability and functions of murine osteosarcoma cells and bone marrow cells, 18,19 and it promoted matrix synthesis by chondrocytes cultured in 3-D collagen sponge and gave the cells better physiological conditions, resulting in their enhanced ability for secretion. 20 All these results suggest the feasibility of the perfusion method for cell attachment in 3-D scaffolds.…”

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confidence: 99%

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

et al. 2005

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The objective of this study was to obtain fundamental knowledge about in vitro culture systems to enhance the proliferation and differentiation of mesenchymal stem cells (MSCs) in collagen sponge reinforced by the incorporation of poly(glycolic acid) (PGA) fiber. A collagen solution with PGA fiber homogeneously localized at PGA:collagen weight ratios of 0.67, 1.25, 2.5, and 5 was freezedried, followed by cross-linking of combined dehydrothermal, glutaraldehyde, and ultraviolet treatment. Scanning electron microscopy revealed that collagen sponges exhibited homogeneous and interconnected pore structures with an average size of 180 microm, irrespective of PGA fiber incorporation. When rat MSCs were seeded into collagen sponge with or without PGA fiber incorporation, more attached cells were observed in collagen sponge incorporating PGA fiber than in collagen sponge without PGA fiber incorporation, irrespective of the PGA:collagen ratio. The proliferation and osteogenic differentiation of MSCs in PGA-reinforced sponge at a weight ratio of 5 were greatly influenced by the culture method and growth conditions. Alkaline phosphatase (ALP) activity and osteocalcin content of MSCs cultured in PGA-reinforced sponge by the perfusion method became maximum at a flow rate of 0.2 mL/min, although they increased with culture time period. It may be concluded that appropriate perfusion conditions enable MSCs to positively improve the extent of proliferation and differentiation.

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confidence: 99%

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

et al. 2005

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“…Moreover, perfusion culture experiments gave new insights in the spatial development as well of renal microvasculature and glomeruli (Kloth et al , 1995(Kloth et al , 1998aKloth and Suter-Crazzolara 2000) as even of intact gastric glands (Kloth et al 1998b). In other experiments it was investigated to what extent regeneration can be influenced by engineered microvessels (Frerich et al 2006(Frerich et al , 2008 or isolated endothelial cells (Bakowsky et al 2005;Hayashi et al 2009).…”

Section: Featuring Development Of Epitheliamentioning

confidence: 99%

“…In analogy, only three papers were found dealing with the regeneration of muscular tissue in combination with the MINUSHEET Ò perfusion culture system. In detail, when a layer of gastric mucosa was mounted in a tissue carrier and kept in a perfusion culture container, it was observed that not only gastric glands but also smooth muscular tissue are developing within the lamina propria (Kloth et al 1998b). Further on, when formation of vessels in brain was investigated, the seeding of cerebral pericytes on selected biomaterials resulted in a high expression of site-specific pericytic aminopeptidase N/pAPN (Ramsauer et al 1998).…”

Section: Development Of Muscular Tissuementioning

confidence: 99%

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

Minuth

Denk

2015

Cytotechnology

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To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET Ò tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.

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“…Since a perfusion culture method facilitates the continuous exchange of medium and the constant removal of metabolic wastes, it has been used for several of cells [34,35,36,37,38,39]. Perfusion culture methods enhanced the viability and functions of murine osteosarcoma cells and bone marrow cells while it promoted matrix synthesis by chondrocytes cultured in a 3D collagen sponge and gave the cells better physiological conditions [40,41], resulting in their enhanced ability for secretion [42].…”

Section: 3d Technology For Development Of Tissue Engineeringmentioning

confidence: 99%

Development of 3D in Vitro Technology for Medical Applications

Hosseinkhani

2014

IJMS

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In the past few years, biomaterials technologies together with significant efforts on developing biology have revolutionized the process of engineered materials. Three dimensional (3D) in vitro technology aims to develop set of tools that are simple, inexpensive, portable and robust that could be commercialized and used in various fields of biomedical sciences such as drug discovery, diagnostic tools, and therapeutic approaches in regenerative medicine. The proliferation of cells in the 3D scaffold needs an oxygen and nutrition supply. 3D scaffold materials should provide such an environment for cells living in close proximity. 3D scaffolds that are able to regenerate or restore tissue and/or organs have begun to revolutionize medicine and biomedical science. Scaffolds have been used to support and promote the regeneration of tissues. Different processing techniques have been developed to design and fabricate three dimensional scaffolds for tissue engineering implants. Throughout the chapters we discuss in this review, we inform the reader about the potential applications of different 3D in vitro systems that can be applied for fabricating a wider range of novel biomaterials for use in tissue engineering.

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Gastric epithelium under organotypic perfusion culture

Cited by 16 publications

References 15 publications

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

Development of 3D in Vitro Technology for Medical Applications

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