Gastric cancer – Authors' reply

Hartgrink, H.H.; Jansen, Edwin P.M.; Grieken, Nicole C.T. van; Velde, Cjh van de

doi:10.1016/s0140-6736(09)61948-6

Cited by 14 publications

(5 citation statements)

References 5 publications

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“…Gastric cancer (GC) is one of the leading causes of cancer related mortality globally (1). China has a high prevalence of GC (2), where the incidence ranked second and the mortality ranked third for all types of cancers (3). Although comprehensive studies of GC have been performed, the mechanisms of its development, progression, metastasis and invasion remain largely unclear, which greatly limits GC therapeutic treatments.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑9 inhibits gastric cancer cell proliferation and migration by targeting neuropilin‑1

et al. 2019

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Gastric cancer (GC) is a global health problem with poor clinical outcomes. The mechanism of its development and progression remains largely unclear. The present study investigated the role of microRNA-9 (miR-9-5p) in the development and progression of GC. Overexpression of miR-9-5p led to reduced expression of neuropilin-1 (NRP-1) in GC cells. Dual-luciferase reporter assay results indicated that miR-9-5p directly targeted NRP-1. Furthermore, overexpression of miR-9-5p in GC cells increased the expression of mesenchymal markers, N-cadherin and vimentin, and decreased the expression of epithelial markers, E-cadherin and β-catenin. Overexpression of miR-9-5p inhibited GC cell proliferation, migration and invasion, and increased the sensitivity of GC cells to the anti-cancer drug cisplatin. By contrast, the opposite effects were observed in GC cells following downregulation of miR-9-5p. Taken together, the present findings suggested that miR-9-5p suppressed NRP-1 expression and inhibited GC cell proliferation and invasion. In addition, miR-9-5p overexpression attenuated GC cell resistance to anti-cancer drugs, which highlighted the potential of miR-9-5p as a target for the treatment of GC.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‑9 inhibits gastric cancer cell proliferation and migration by targeting neuropilin‑1

et al. 2019

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“…Radiotherapy is the major loco-regional control method for unresectable GC. Unfortunately, intrinsic radio-resistance of cells results in failure of radiotherapy in numerous patients (5). The guidelines of the National Comprehensive Cancer Network recommend radiotherapy as a standard therapy for patients with GC.…”

Section: Introductionmentioning

confidence: 99%

DNA‑PKcs inhibitor increases the sensitivity of gastric cancer cells to radiotherapy

et al. 2019

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Gastric cancer (GC) is a severe public health problem worldwide, particularly in China. Radiotherapy is the main locoregional treatment for various types of unresectable tumor, including GC. However, many patients fail to respond to radiotherapy due to the intrinsic radioresistance of cancer cells. This study was designed to investigate the effects and potential mechanism of radiosensitization associated with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) inhibitor in human GC cell lines in vitro . Among the six GC cell lines (SGC7901, HGC-27, MKN45, MKN74, BGC823 and MGC803) that were exposed to increasing doses of IR (0, 2, 4, 6 and 8 Gy), the mean lethal dose and quasi-threshold dose measurements indicated that BGC823 and MGC803 were relatively insensitive to ionizing radiation (IR). IR induced significant elevation of γ H2A histone family member X (γH2AX) in MKN45 cells compared with BGC823 cells. DNA-PKcs and phospho-DNA-PKcs protein levels were increased in BGC823 and MGC803 cells compared with other GC cell lines (SGC7901, HGC-27, MKN45 and MKN74). DNA-PKcs inhibition led to increased sensitivity of BGC823 and MGC803 cells to IR. NU7441 increased γH2AX expression in the nuclei of BGC823 cells following IR. Combination of DNA-PKcs and CK2 inhibition further increased the sensitivity of GC cells to IR. The combination of NU7441 and CX4945 increased γH2AX expression in the nucleus of BGC823 cells following IR compared with treatment with NU7441 alone. Taken together, the findings suggest that DNA-PKcs inhibitor increased the sensitivity of radioresistant BGC823 and MGC803 cells to radiotherapy through the cleaved-caspase3/γH2AX signaling pathway, thus presenting a potential treatment method for GC.

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“…Gastric cancer has been proven to be one of the most common and most commonly diagnosed malignant tumors worldwide (1), with a low 5-year survival rate (2). As is already known, early diagnosis and effective therapeutic targets are critical factors influencing clinical treatment and prognosis.…”

Section: Introductionmentioning

confidence: 99%

Flap endonuclease 1 is a promising candidate biomarker in gastric cancer and is involved in cell proliferation and apoptosis

Wang

Xie

Daorong

2014

International Journal of Molecular Medicine

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As a DNA repair protein, flap endonuclease 1 (FEN1), a structure-specific 5' nuclease, plays pivotal roles in the maturation of Okazaki fragments, long-patch base excision repair, restarting of stalled replication forks and telomere maintenance. FEN1 possesses 5' endonuclease, 5' exonuclease and gap-endonuclease activities, which render it an essential node in maintaining genome fidelity. The aim of this study was to investigate the association between the expression level of FEN1 and gastric cancer and to explore the role of FEN1 in carcinogenesis and the progression of gastric cancer. The mRNA and protein expression of FEN1 in 42 matched pairs of human gastric tumor tissues and corresponding normal tissues were measured by semiquantitative reverse transcription-PCR and immunohistochemical staining. FEN1 expression was downregulated in the SGC-7901 gastric cancer cells following transfection with siRNA targeting the FEN1 gene. Western blot analysis was used to evaluate the protein expression of FEN1 in SGC-7901 human gastric cancer cells in order to verify the transfection efficiency of FEN1 siRNA. Moreover, cell proliferation was analyzed by MTS assay. The apoptosis of the cells was determined by flow cytometry. Our results revealed that FEN1 was overexpressed in gastric cancer in comparison to the corresponding normal gastric tissues (P<0.01). We further confirmed that FEN1 expression has a positive correlation with the degree of differentiation (P=0.027), lymphatic metastasis (P=0.001), tumor size (P=0.026) and TNM stage (P=0.020) of gastric cancer. A high FEN1 expression in SGC-7901 cells can be effectively downregulated by siRNA constructed to target the FEN1 gene. Moreover, the inhibition of FEN1 expression suppressed the proliferation and induced the apoptosis of SGC-7901 cells. Taken together, our results indicate that FEN1 may be a promising biomarker for the diagnosis of gastric cancer and individual therapy.

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Gastric cancer – Authors' reply

Cited by 14 publications

References 5 publications

MicroRNA‑9 inhibits gastric cancer cell proliferation and migration by targeting neuropilin‑1

MicroRNA‑9 inhibits gastric cancer cell proliferation and migration by targeting neuropilin‑1

DNA‑PKcs inhibitor increases the sensitivity of gastric cancer cells to radiotherapy

Flap endonuclease 1 is a promising candidate biomarker in gastric cancer and is involved in cell proliferation and apoptosis

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