The article presents results of work aimed at determining the influence of hydrocarbon gasoline used to composed E85 ethanol fuel on the properties of engine oil used in spark ignition Flex Fuel automobile engine. For this purpose, two ethanol fuels were composed which consisting of 85% (V/V) ethanol and 15% (V/V) conventional hydrocarbon gasoline, which was obtained from two producers. As an object of the study, an engine oil was recommended for this type of engine lubrication, the SAE 5W-30 viscosity grades and ACEA A5/B5 quality grade engine oil was selected. Monitoring of the properties of the subjected oil was carried out before and after 300 hours of engine operation. Test samples of engine oil retained their rheological properties at levels corresponding to their viscosity grade.