Gas-stabilizing nanoparticles for ultrasound imaging and therapy of cancer

Sabuncu, Sinan; Yıldırım, Adem

doi:10.1186/s40580-021-00287-2

Cited by 17 publications

(13 citation statements)

References 112 publications

(202 reference statements)

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“…Nevertheless, the application in tumor imaging and treatment is limited due to the large size, poor stability, and short cavitation activity of microbubbles. 118,119 USI that could be activated by tumor specific signals has entered people's vision in recent years, especially the new responsive ultrasound contrast agent based on the bioactive inorganic nanomaterials, which has better circulatory stability and tumor accumulation, making this kind of unique nanomaterials suitable for activable USI. B-mode USI is an important anatomical technique in clinic, which shows the anatomical variation of tissues.…”

Section: Activated Ultrasound Imaging Of Bioactive Inorganic Nanomate...mentioning

confidence: 99%

Bioactive inorganic nanomaterials for cancer theranostics

2023

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Section: Activated Ultrasound Imaging Of Bioactive Inorganic Nanomate...mentioning

confidence: 99%

Bioactive inorganic nanomaterials for cancer theranostics

2023

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“…In recent years, several solid nanoparticles that can stabilize small gas pockets (i.e., nanobubbles) on their surfaces have been developed as contrast agents for ultrasound imaging and cavitation agents for ultrasound-based therapies. [1][2][3][4][5][6] Such A key challenge in producing biodegradable GSNs is the requirement of hydrophobicity for gas stabilization, which can also prevent nanoparticle degradation. One common method to prepare GSNs is to use inherently hydrophobic polymer nanoparticles such as divinylbenzene crosslinked poly(methyl methacrylate) or polytetrafluoroethylene, which are nondegradable.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, several solid nanoparticles that can stabilize small gas pockets (i.e., nanobubbles) on their surfaces have been developed as contrast agents for ultrasound imaging and cavitation agents for ultrasound‐based therapies. [ 1–6 ] Such nanoparticles, namely gas‐stabilizing nanoparticles (GSNs), produce micron‐sized bubbles when activated with sufficiently intense ultrasound pulses delivered using focused ultrasound (FUS) or standard ultrasound imaging transducers. The bubbles generated by GSNs enhance the contrast of ultrasound images as they are strong scatterers of ultrasound beams.…”

Section: Introductionmentioning

confidence: 99%

Protein‐Coated Biodegradable Gas‐Stabilizing Nanoparticles for Cancer Therapy and Diagnosis Using Focused Ultrasound

Sabuncu

Mira

Quentel

et al. 2022

Adv Materials Inter

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nanoparticles, namely gas-stabilizing nanoparticles (GSNs), produce micron-sized bubbles when activated with sufficiently intense ultrasound pulses delivered using focused ultrasound (FUS) or standard ultrasound imaging transducers. The bubbles generated by GSNs enhance the contrast of ultrasound images as they are strong scatterers of ultrasound beams. [7][8][9][10][11] In addition, the non-stabilized bubbles rapidly collapse (i.e., inertial cavitation) to release intense mechanical energy and induce various biological effects in the tissue, from permeabilization of cell membranes to ablation of cells. [4,5,12] Thus, GSNs have also been used to enhance the outcomes of therapeutic ultrasound applications such as drug delivery, antivascular therapy, and thrombolysis. [1,[13][14][15][16][17][18][19][20][21] Conventional ultrasound contrast/cavitation agents include lipid-, protein-, or polymer-stabilized gas microbubbles and micro/nanodroplets of low-boiling point perfluorocarbons, which can be vaporized to form acoustically active microbubbles. [4,[22][23][24] Compared with these more conventional ultrasound contrast agents, GSNs pose several advantages: small size (down to ≈50 nm), excellent storage and in vivo stability, and high and durable cavitation activity. [2,9,[13][14][15]25] However, the non-degradable nature of GSNs is a concern as such nanoparticles can accumulate and potentially induce toxicity in the liver, spleen, and bone marrow. [26][27][28][29] Surface-engineered hydrophobic nanoparticles that can stabilize small gas pockets on their surfaces (i.e., gas-stabilizing nanoparticles, GSNs) have been recently shown to be excellent contrast and cavitation agents for ultrasound theranostics. However, previously developed GSNs are not biodegradable, which limits their clinical translation potential. Here the development of biodegradable GSNs is shown by coating hydrophobically modified mesoporous silica nanoparticles with different protein solutions. It is found that these novel GSNs retain strong cavitation activity while rapidly degrading in simulated body fluid (SBF) or in vivo in days or a few weeks, respectively. Interestingly, GSNs coated with other stabilizing layers, Pluronic F127 polymer or phospholipids, demonstrated significantly slower degradation rates with only partial degradation even after a month of incubation in SBF. Next, it is shown that these biodegradable GSNs can be used to ablate tumor xenografts at lower ultrasound intensities, thus avoiding the side effects of high-intensity ultrasound. Finally, it is shown that only tumors treated with GSNs and ultrasound can specifically enrich for circulating tumor DNA, which will improve liquid biopsies for understanding tumor heterogeneity and treatment response. Overall, this study details a simple yet effective method for preparing biodegradable GSNs with broad potential for applications in cancer diagnosis and therapy.

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“…7 The most widely used substances are perfluorocarbons (PFH) with low boiling point (56°C) liquid-gas phase transition properties, which are used for synthesizing nanoscale HIFU synergistic agents. [8][9][10][11][12][13][14] Nano drug delivery system has been widely used in the treatment of tumors, [15][16][17][18] but the HIFU synergist developed based on nanomaterials still has some problems of poor targeting and loss to normal tissues. Moreover, HIFU treatment is inseparable from image monitoring, but single ultrasound imaging makes it hard to meet the growing demand for HIFU treatment.…”

Section: Introductionmentioning

confidence: 99%

US/MR Bimodal Imaging-Guided Bio-Targeting Synergistic Agent for Tumor Therapy

Jiang¹,

Wang²,

Tang³

et al. 2022

IJN

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Breast cancer is detrimental to the health of women due to the difficulty of early diagnosis and unsatisfactory therapeutic efficacy of available breast cancer therapies. High intensity focused ultrasound (HIFU) ablation is a new method for the treatment of breast tumors, but there is a problem of low ablation efficiency. Therefore, the improvement of HIFU efficiency to combat breast cancer is immediately needed. This study aimed to describe a novel anaerobic bacteria-mediated nanoplatform, comprising synergistic HIFU therapy for breast cancer under guidance of ultrasound (US) and magnetic resonance (MR) bimodal imaging. Methods: The PFH@CL/Fe 3 O 4 nanoparticles (NPs) (Perfluorohexane (PFH) and superparamagnetic iron oxides (SPIO, Fe 3 O 4 ) with cationic lipid (CL) NPs) were synthesized using the thin membrane hydration method. The novel nanoplatform Bifidobacterium bifidum-mediated PFH@CL/Fe 3 O 4 NPs were constructed by electrostatic adsorption. Thereafter, US and MR bimodal imaging ability of B. bifidum-mediated PFH@CL/Fe 3 O 4 NPs was evaluated in vitro and in vivo. Finally, the efficacy of HIFU ablation based on B. bifidum-PFH@CL/Fe 3 O 4 NPs was studied. Results: B. bifidum combined with PFH@CL/Fe 3 O 4 NPs by electrostatic adsorption and enhanced the tumor targeting ability of PFH@CL/Fe 3 O 4 NPs. US and MR bimodal imaging clearly displayed the distribution of the bio-targeting nanoplatform in vivo. It was conducive for accurate and effective guidance of HIFU synergistic treatment of tumors. Furthermore, PFH@CL/Fe 3 O 4 NPs could form microbubbles by acoustic droplet evaporation and promote efficiency of HIFU ablation under guidance of bimodal imaging. Conclusion:A bio-targeting nanoplatform with high stability and good physicochemical properties was constructed. The HIFU synergistic agent achieved early precision imaging of tumors and promoted therapeutic effect, monitored by US and MR bimodal imaging during the treatment process.

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Gas-stabilizing nanoparticles for ultrasound imaging and therapy of cancer

Cited by 17 publications

References 112 publications

Bioactive inorganic nanomaterials for cancer theranostics

Bioactive inorganic nanomaterials for cancer theranostics

Protein‐Coated Biodegradable Gas‐Stabilizing Nanoparticles for Cancer Therapy and Diagnosis Using Focused Ultrasound

US/MR Bimodal Imaging-Guided Bio-Targeting Synergistic Agent for Tumor Therapy

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