Gas Plasma-Conditioned Ringer’s Lactate Enhances the Cytotoxic Activity of Cisplatin and Gemcitabine in Pancreatic Cancer In Vitro and In Ovo

Liedtke, Kim Rouven; Freund, Eric; Hermes, Maraike; Oswald, Stefan; Heidecke, Claus–Dieter; Partecke, Lars-Ivo; Bekeschus, Sander

doi:10.3390/cancers12010123

Cited by 34 publications

(35 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tumor-toxic injections with plasma-treated liquids have been recently demonstrated in vivo using a model of pancreatic cancer [88]. Finally, another timely question is whether plasma treatment might be able to revert chemotherapeutic resistance, but apart from first studies on drug-transporter expression in cancer cells after plasma treatment [89,90], such studies are yet awaited to come.…”

Section: Discussionmentioning

confidence: 99%

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

et al. 2020

Self Cite

View full text Add to dashboard Cite

Despite recent improvements in cancer treatment, with many of them being related to foster antitumor immunity, tumor-related deaths continue to be high. Novel avenues are needed to complement existing therapeutic strategies in oncology. Medical gas plasma technology recently gained attention due to its antitumor activity. Gas plasmas act via the local deposition of a plethora of reactive oxygen species (ROS) that promote the oxidative cancer cell death. The immunological consequences of plasma-mediated tumor cell death are only poorly understood, however. To this end, we exposed two prostate cancer cell lines (LNCaP, PC3) to gas plasma in vitro, and investigated the immunomodulatory effects of the supernatants in as well as of direct co-culturing with two human myeloid cell lines (THP-1, HL-60). After identifying the cytotoxic action of the kINPen plasma jet, the supernatants of plasma-treated prostate cancer cells modulated myeloid cell-related mitochondrial ROS production and their metabolic activity, proliferation, surface marker expression, and cytokine release. Direct co-culture amplified differentiation-like surface marker expression in myeloid cells and promoted their antitumor-toxicity in the gas plasma over the untreated control conditions. The results suggest that gas plasma-derived ROS not only promote prostate cancer cell death but also augment myeloid cell activity and cytotoxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is conceivable that gas plasma treatment may not only be used for tumor patient palliation, but also as adjuvant or neo-adjuvant therapy during radiotherapy or chemotherapy. This way, the tumor cells might be targeted by several mechanisms simultaneously, as we have recently demonstrated experimentally in other types of cancer cells [72][73][74].…”

Section: Clinical Studiesmentioning

confidence: 88%

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

et al. 2020

Self Cite

View full text Add to dashboard Cite

Despite progress in oncotherapy, cancer is still among the deadliest diseases in the Western world, emphasizing the demand for novel treatment avenues. Cold physical plasma has shown antitumor activity in experimental models of, e.g., glioblastoma, colorectal cancer, breast carcinoma, osteosarcoma, bladder cancer, and melanoma in vitro and in vivo. In addition, clinical case reports have demonstrated that physical plasma reduces the microbial contamination of severely infected tumor wounds and ulcerations, as is often seen with head and neck cancer patients. These antimicrobial and antitumor killing properties make physical plasma a promising tool for the treatment of head and neck cancer. Moreover, this type of cancer is easily accessible from the outside, facilitating the possibility of several rounds of topical gas plasma treatment of the same patient. Gas plasma treatment of head and neck cancer induces diverse effects via the deposition of a plethora of reactive oxygen and nitrogen species that mediate redox-biochemical processes, and ultimately, selective cancer cell death. The main advantage of medical gas plasma treatment in oncology is the lack of adverse events and significant side effects compared to other treatment modalities, such as surgical approaches, chemotherapeutics, and radiotherapy, making plasma treatment an attractive strategy for the adjuvant and palliative treatment of head and neck cancer. This review outlines the state of the art and progress in investigating physical plasma as a novel treatment modality in the therapy of head and neck squamous cell carcinoma.

show abstract

“…One such innovative procedure, which complements the intraoperative treatment of tumor tissue, could be CAP treatment of the resection margins. The antitumor effects of CAP have already been demonstrated in some cancer entities such as melanoma and pancreatic and breast carcinomas [23][24][25]. Many of the cellular effects, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) response as products of CAP treatment, have been already described in detail [26].…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

Haralambiev

Nitsch

Jacoby

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Chondrosarcoma is the second most common malign bone tumor in adults. Surgical resection of the tumor is recommended because of its resistance to clinical treatment such as chemotherapy and radiation therapy. Thus, the prognosis for patients mainly depends on sufficient surgical resection. Due to this, research on alternative therapies is needed. Cold atmospheric plasma (CAP) is an ionized gas that contains various reactive species. Previous studies have shown an anti-oncogenic potential of CAP on different cancer cell types. The current study examined the effects of treatment with CAP on two chondrosarcoma cell lines (CAL-78, SW1353). Through proliferation assay, the cell growth after CAP-treatment was determined. A strong antiproliferative effect for both cell lines was detected. By fluorescein diacetate (FDA) assay and ATP release assay, alterations in the cell membrane and associated translocation of low molecular weight particles through the cytoplasmic membrane were observed. In supernatant, the non-membrane-permeable FDA and endogenously synthesized ATP detected suggest an increased membrane permeability after CAP treatment. Similar results were shown by the dextran-uptake assay. Furthermore, fluorescence microscopic G-/F-actin assay was performed. G- and F-actin were selectively dyed, and the ratio was measured. The presented results indicate CAP-induced changes in cell membrane function and possible alterations in actin-cytoskeleton, which may contribute to the antiproliferative effects of CAP.

show abstract

Gas Plasma-Conditioned Ringer’s Lactate Enhances the Cytotoxic Activity of Cisplatin and Gemcitabine in Pancreatic Cancer In Vitro and In Ovo

Cited by 34 publications

References 65 publications

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

Contact Info

Product

Resources

About