Gas-Phase Bioreactors

Influence of Lipase Immobilization Mode on Ethyl Acetate Hydrolysis in a Continuous Solid–Gas Biocatalytic Membrane Reactor

“…This finding is in good agreement with literature data. 10,11,15 On the other hand, water could have a negative effect on the biocatalyst because, at a high temperature, it can denaturate it. 9 However, no enzyme denaturation can be supposed in the studied systems, since for the investigated molar flow rate range, no decreasing of reaction rate was observed.…”

“…4,10 They include higher thermostability of the dehydrated enzyme with respect to the hydrated form, reduction of microbial contamination, reduction of byproducts formation, as well as improvements in mass transfer and product recovery. 10,11 In the literature, several bioreactor configurations (fluidized bed, membrane bioreactor, air lift, etc. 10,11 ) for solid−gas biocatalysis are reported, in which different biocatalysts (hydrogenase, 12 alcohol oxidase, 13 carbonic anhydrase, 14 and lipases from different sources and cells) immobilized on different supports 10 (alumina silicate, molecular sieves, glass beads resins, and polymeric supports) or free in solution were used.…”

“…10,11 In the literature, several bioreactor configurations (fluidized bed, membrane bioreactor, air lift, etc. 10,11 ) for solid−gas biocatalysis are reported, in which different biocatalysts (hydrogenase, 12 alcohol oxidase, 13 carbonic anhydrase, 14 and lipases from different sources and cells) immobilized on different supports 10 (alumina silicate, molecular sieves, glass beads resins, and polymeric supports) or free in solution were used. The design of a solid−gas bioreactor is quite simple, and an important parameter in these systems is the water−enzyme interaction.…”

Biological removal of gas‐phase H2S in hollow fibre membrane bioreactors

“…Efficient H 2 S removal in a bioreactor depends on some key components: packing material for microbial attached growth, 4 gas-liquid mass transfer, 3 biofilm characteristics and microbial community composition, 5 and operating conditions, such as inlet concentration and loading rate, 3,12 gas flow rate corresponding to EBRT, 6,12 pH and temperature. 36 This is, to the authors' knowledge, the first report on the use of a submerged HFM module based HFMB configuration for H 2 S gas treatment. In contrast, hollow fibre membrane contactors (HFMCs) are well known in H 2 S treatment.…”

“…This study showed that the HFMB reactor configuration can efficiently treat gas‐phase H 2 S up to an ILR of ~17.0 g m −3 h −1 at an EBRT of 187 s (Figs 2 and 3). Efficient H 2 S removal in a bioreactor depends on some key components: packing material for microbial attached growth, 4 gas–liquid mass transfer, 3 biofilm characteristics and microbial community composition, 5 and operating conditions, such as inlet concentration and loading rate, 3,12 gas flow rate corresponding to EBRT, 6,12 pH and temperature 36 …”