Gas-liquid chromatography of bacterial fatty acids with a fused-silica capillary column

Moss, C W; Dees, S B; Guerrant, G O

doi:10.1128/jcm.12.1.127-130.1980

Cited by 56 publications

(25 citation statements)

References 8 publications

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“…Under these conditions, the unsaturated acids disappeared and the equivalent saturated methyl esters increased proportionally. Methyl esters of hydroxy fatty acids were confirmed by trifiuoroacetylation (Moss 1981). At completion of the reaction, the hydroxy acids disappeared and diester derivatives with shorter retention times appeared.…”

Section: Confirmatory Testsmentioning

confidence: 89%

“…Gas-liquid chromatography has become a more useful analytical tool with the advent of fused-silica capillary columns (Moss et al 1980). In general, capillary column can resolve and detect fatty acid components comprising as little as 0.01 % of total fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…Unsaturated fatty acids were confirmed by hydrogenation of methyl esters for 15 min by procedures described by Moss et al (1980). Under these conditions, the unsaturated acids disappeared and the equivalent saturated methyl esters increased proportionally.…”

Section: Confirmatory Testsmentioning

confidence: 99%

“…As decay-causing bacteria, E. carotovora and chrysanthemi, and also rhapontid, occur on rotting plant tissues and since they are not always host specific, can be isolated from the same field samples (PEROMBELON andKELMAN 1980, TOWNER andBERAHA 1976). Although definitive physiological and biological tests can be used for identification of these isolates, more rapid analytical techniques such as fatty acid analysis are being developed (Moss 1981).…”

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confidence: 99%

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Differentiation of the Soft‐Rotting Erwinias (the Carotovora Group) by Fatty Acid Composition

Wells

Moline

1991

Journal of Phytopathology

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About 90 % of total cellular fatty acids in E. carotovora, grown on KB medium for 1 day at 28°C, were the saturated, even‐carbon straight chains 12 : 0 (5.9%), 14 : 0 (1.7%) and 16 : 0 (30.1 %), and the unsaturated 16 : 1 (36.6%) and 18 : 1 (15.0%) fatty acids. Other components were the hydroxy‐substituted 3‐OH 14 : 0 (5.3%) and 21 minor fatty acids each occurring less than 0.1 % of the total — 14 of them reported herein for the first time in Erwinia. The ratio of 16 : 1/18 : 1 in KB‐grown cells was as useful in differentiating subspecies of E. carotovora as previously reported by other workers for TSA‐grown cells. A comparison of fatty acid profiles of E. carotovora on 4 different media, KB, TSA, NA and PDA, indicated that on KB there was the greatest proportion of Class A and C fatty acids, and the highest number of detectable components. Significant differences were noted in the 5 major fatty acids and in cyclic fatty acids among the 4 species of the carotovora group –E. carotovora, E. chrysanthemi, E. rhapontici and E. cypripedii. These differences could be expressed as algorithms that, when used in sequential dichotomous steps, could differentiate the 4 species.

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Section: Confirmatory Testsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Confirmatory Testsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Differentiation of the Soft‐Rotting Erwinias (the Carotovora Group) by Fatty Acid Composition

Wells

Moline

1991

Journal of Phytopathology

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“…Confirmation of the identify of the minor fatty acids, especially those occurring at concentrations of 0.1 % or less, was by chemical tests. The presence of unsaturated fatty acids was confirmed by hydrogenation of methyl esters for 15 min by procedures previously described (Moss 1981). Under these conditions, the unsaturated acids disappeared and the equivalent saturated methyl esters increased proportionally.…”

Section: Gas-liquid Chromatography and Mass Spectrometrymentioning

confidence: 92%

Fatty Acid Composition of Pseudomonas syringae pv. savastanoi

Wells

Casano

Surico

1991

Journal of Phytopathology

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Over 85 % of total cellular fatty acids of 30 strains of P. syringae pv. savastanoi, grown for one day at 28 °C on King's medium B (KB) agar, were 12 : 0 (5.0 %), 16 : 0 (27.5 %), 16 : 1 (36.7 %) and 18 : 1 (16.8 %). Three hydroxy-substituted fatty acids comprised 7.2 % of the total and 22 other minor components, each occurring at concentrations of less than 1 %, comprised an additional 4 %. Three percent were unidentified components. Cells grown for 3 and 6 days on KB agar contained lower concentrations of the unsaturated 16 : 1 (30.4 and 21.1 %, respectively), and higher concentrations of branched-chain and cyclopropane fatty acids than one-day old cells. No consistent differences in fatty acid composition could be detected between virulent and avirulent strains, nor between pv. savastanoi and other pathovars of P. syringae. However, when cells were grown on a chemicallydefined medium for 6 days, concentrations of 16 : 0 and a tentatively-identified 17-carbon hydroxy fatty acid were higher, and those of 12 : 0 and 16 : 1 were lower in strains from Fraxinus than from Olea. P. fluorescens (7 strains) and P. viridiflava (6 strains) could be differentiated from each other but not from P. syringae. ZusammenfassungFettsaurezusammensetzung von Pseudomonas syringae pv. savastanoi tJber 85 % der gesamten zelluiaren Fettsauren bei 30 Stammen von Pseudomonas syringae pv. savastanoi, die einen Tag auf King's-medium-B (KB)-Agar bei 28 °C kultiviert worden waren, waren 12 : 0 (5,0 %), 16 : 0 (27,5 %), 16 :1 (36,7 %) und 18 : 1 (16,8 %). Drei Hydroxy-substitutierte Fettsauren trugen weitere 7,2 % dem gesamten Bild bei, und weitere 4 % bestanden aus 22 unbedeutenden Komponenten, alle bei Konzentrationen unter 1 %. Nicht bestimmte Komponenten kamen auf 3 %. Zellen, die 3 bzw. 6 Ts^e auf KB-Agar wuchsen, enthielten niedrigere Konzentrationen der ungesattigten 16 :1 (30,4 bzw. 21,1 %), und hohere Konzentrationen der verzweigt-kettigen U.S. Copyright Cleaiance Center Code Statement: 0931-1785/91/3302-0152$02.50/0 Fatty Acid Composition of Pseudomonas syringae pv. savastanoi 153 und Cyclopropan-Fettsauren als einen Tag alte Zellen. Es konnten keine konstanten Unterschiede der Fettsaurezusammensetzungen von virulenten und avirulenten Stammen festgestellt werden, das gleiche gilt zwischen pv. savastanoi und anderen P. syringae-'P&ikovzTen. Wenn die Zellen 6 Tage lang auf einem chemisch definierten Nahrmedium wuchsen, wurden hohere Konzentrationen von 16 : 0und einer zunachst als 17-Kohlenstoffhydroxy bestimmten Fettsaure ermittelt, dagegen waren die Konzentrationen der 12 : 0-und 16 : 1-Fettsauren niedriger in Stammen von Fraxinus als von Olea. Eine Differenzienmg zwischen P. fluorescens (7 Stamme) und P. viridiflava (6 Stamme) konnte ermittelt werden, jedoch nicht zwischen P. syringae und den anderen Spezies.

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Janthinobacterium

Gillis¹,

Logan²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Jan.thin.o.bac.te' ri.um . L. adj. janthinus violet‐colored; Gr. n. bakterion a small rod; M.L. neut. n. Janthinobacterium a small, violet‐colored rod. Proteobacteria / Betaproteobacteria / Burkholderiales / Oxalobacteraceae / Janthinobacterium Round‐ended , straight rods , sometimes slightly curved , 0 . 8–1 . 5 × 1 . 8–6 . 0 μ m , occurring singly and sometimes in pairs or short chains. Definite capsules are not evident, but intercellular slime may be produced. No resting stages are known. Gram negative, occasionally showing bipolar or barred staining and lipid inclusions. Motile . Strict aerobes . Growth occurs from 4 ° C to about 30 ° C , with optimum growth at around 25 ° C . Optimal pH 7–8, with no growth below pH 5. Many strains produce the violet pigment violacein , but strains producing partly pigmented or nonpigmented colonies are often encountered. Colonies on routine solid media are low convex and round. Aerobic , having a strictly respiratory metabolism with oxygen as the terminal electron acceptor . Oxidase and catalase positive . Chemoorganotrophs. Growth occurs on ordinary peptone media. Citrate and ammonium ions can be used as sole carbon and nitrogen sources, respectively. Small amounts of acid, but no gas, are produced from glucose and some other carbohydrates. The genus is characterized by the following major phospholipids : phosphatidyl ethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as polar lipids. The major fatty acids are C 16:0 , C 16:1 ω7c , and C 17:0 cyclo . Only C 10:0 3OH and C 12:0 2OH are synthesized; the C 10:0 3OH are ester‐ and (presumably) amide‐linked, and the C 12:0 2OH fatty acids are (presumptively) ester‐linked. Q‐8 is the major respiratory lipoquinone. Occur in soil and water and are common in temperate climates and cold climates, but can also be isolated from diseased mushroom tissue. The mol % G + C of the DNA is : 61–67. Type species : Janthinobacterium lividum (Eisenberg 1891) De Ley, Segers and Gillis 1978, 164 ( Chromobacterium lividum Bergey, Harrison, Breed, Hammer and Huntoon 1923a, 119; Bacillus lividus Eisenberg 1891, 81.)

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Gas-liquid chromatography of bacterial fatty acids with a fused-silica capillary column

Cited by 56 publications

References 8 publications

Differentiation of the Soft‐Rotting Erwinias (the Carotovora Group) by Fatty Acid Composition

Differentiation of the Soft‐Rotting Erwinias (the Carotovora Group) by Fatty Acid Composition

Fatty Acid Composition of Pseudomonas syringae pv. savastanoi

Janthinobacterium

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