Gas-Liquid Chromatography of Amino Acid Derivatives

Johnson, D.; Scott, S. J.; Meister, Alton

doi:10.1021/ac60174a007

Cited by 88 publications

(16 citation statements)

References 10 publications

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“…Synthesis of derivative8for gas-liquid chromatography Amino acids. The N-acetyl-n-pentyl esters of the amino acids were produced according to the method of Johnson, Scott & Meister (1961).…”

Section: Methodsmentioning

confidence: 99%

Mechanism of conversion of aspartate into glutamate in cerebral-cortex slices

Simon¹,

Drori²,

Cohen³

1967

Biochemical Journal

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1. When slices of guinea-pig cerebral-cortex slices are incubated with [U-(14)C]-aspartate and non-radioactive glucose as substrates, the specific radioactivities of the citric acid-cycle intermediates are lower than that of the glutamate isolated from the same vessels. 2. Glutamate was significantly labelled when [1-(14)C]-aspartate and glucose were present in the incubation medium. These results would not be expected on the basis of simple conversion of aspartate into glutamate through the citric acid cycle, since the C-1 position of oxaloacetate is decarboxylated in the conversion of isocitrate into alpha-oxoglutarate. 3. It appears that aspartate is converted into glutamate by citric acid-cycle mechanisms; however, the carbon ;skeleton' is not immediately condensed with acetyl-CoA to form citrate but first follows the cycle in a reverse direction to fumarate or succinate and then proceeds in the forward direction. 4. The conversion of aspartate into glutamate appears to be compartmentalized.

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Section: Methodsmentioning

confidence: 99%

Mechanism of conversion of aspartate into glutamate in cerebral-cortex slices

Simon¹,

Drori²,

Cohen³

1967

Biochemical Journal

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“…The developed gas-chromatographic method of analysis offers the following: less initial instrument cost, more instrument versatility, more rapid analysis, and greater sensitivity. 15. Quantitative reference standard solutions of N-trifluoroacetyl n-butyl amino acid esters available from Regis Chemical Co., 1101 N. FrankIin St., Chicago, Ill. 60610.…”

Section: Removalmentioning

confidence: 99%

“…One of the first to recognize the advantages of N-substituted esters of amino acids as derivatives for gas chromatography was Youngs (38), who in 1959 reported that chromatographic peaks had been obtained for the N-acetyl n-butyl esters of glycine, alanine, valine, leucine, isoleucine, and proline. Johnson et al (15,16) investigated the preparation and gas-chromatographic separation of the N-acetyl n-amyl esters. Chromatographic peaks were obtained for the derivatives of 36 amino acids, including 18 of the common protein amino acids, but difficulties were experienced with tryptophan, histidine, cystine, and arginine.…”

mentioning

confidence: 99%

Quantitative Analysis of the Twenty Natural Protein Amino Acids by Gas-Liquid Chromatography

1967

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“…1C1/water, 65.0 : 1.6.6 : 18.4 were _ '1. 4 used as solvent systems according to the method of Fink, Klein and Fink. Serine, cystine, leucine, giutamic acid, phenylalanine, alanine, and tyrosJ.te 'were found present.…”

Section: Piro C Ed1) R Ementioning

confidence: 99%

Differentiation of Amino Acids by Gas-Liquid Chromatography of Their Pyrolysis Products

Winter¹,

Albro²

1963

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Gas-Liquid Chromatography of Amino Acid Derivatives

Cited by 88 publications

References 10 publications

Mechanism of conversion of aspartate into glutamate in cerebral-cortex slices

Mechanism of conversion of aspartate into glutamate in cerebral-cortex slices

Quantitative Analysis of the Twenty Natural Protein Amino Acids by Gas-Liquid Chromatography

Differentiation of Amino Acids by Gas-Liquid Chromatography of Their Pyrolysis Products

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