Gas-liquid chromatographic method for the determination of peracids in the presence of a large excess of hydrogen peroxide

Abstract

The oxidation of organic sulphides is very fast with peracids and very slow with hydrogen peroxide. Thus, addition of an excess of methyl p-tolyl sulphide to a mixture of an organic peracid and hydrogen peroxide results in the formation of methyl p-tolyl sulphoxide equimolar with the peracid. The gas-chromatographic determination of the residual sulphide, or of the produced sulphoxide, affords a quantitative evaluation of the peracid present in the mixture. The method has been applied to the determination of m-chloroperbenzoic acid and peracetic and perpropionic acids.

Fluorescent Assay for Directed Evolution of Perhydrolases

“…1-3 [17][18][19] ), chromatography methods ( Table 1: no. 4-6 [20][21][22] ), absorbance ( Table 1: no. 7-11 [23][24][25][26][27] ), and fluorescence ( 28 ) detection.…”

A High-Throughput Microtiter Plate Based Method for the Determination of Peracetic Acid and Hydrogen Peroxide

“…Methods for the separation, derivatization and detection of these peroxides, such as gas chromatography [14] , [15] , [16] and HPLC [17] , [18] , [19] have been developed. While these chromatographic methods are able to quantitate both peracetic acid and hydrogen peroxide, like nearly all chromatographic methods, large numbers of solutions cannot be rapidly processed.…”

Photometric and Gas-Chromatographic Determination of Hydrogen Peroxide and Peroxybutanoic Acid in Oxidized Butanoic Acid

“…The observed decrease in the total concentration of peroxide compounds is detected by conventional methods, most often, by iodometry [2, 10]. It was proposed that peroxy acids be determined from the yield of a sulfur-containing reagent, sulfoxide and (or) sulfone [11][12][13].H 2 O 2 and peroxy acids are difficult to determine quantitatively by available methods in organic compounds oxidized by molecular oxygen or by a mixture of H 2 O 2 and carboxylic acid, because the composition of the products of these reactions is rather complex. It is known that peroxide and oxygen-containing compounds can distort the results of analytical determinations of the products of liquid-phase oxidation [4].…”

“…The molecular mass of catalase was taken equal to 250 000 [2]. The method for obtaining peroxydodecanoic acid is reported in [12]. We used a preparation that contained 99.3 ± 0.3% of the peroxy acid.…”

“…The observed decrease in the total concentration of peroxide compounds is detected by conventional methods, most often, by iodometry [2,10]. It was proposed that peroxy acids be determined from the yield of a sulfur-containing reagent, sulfoxide and (or) sulfone [11][12][13].…”