Gas-liquid chromatographic determination of cocaine and benzoylecgonine in urine

Abstract

ures shown in Table VII are obtained (C13, Cn, C9 cardanols have not been taken into account). The total derivable cardanol (83.56%) is slightly higher than if only the C15 component had been considered. Expressed in terms of the same % cardol (14.34%), the total (C15 and C17) cardanol is 85.41%.Pillay (20) thought that "a thorough diagnosis of CNSL by all known instrumental methods is still to come". The stationary phase PEGA used in the present method for the component phenols and also in the TLC/GLC method for the unsaturated constituents (13) provides a general method for the total analysis of CNSL and for the study of component phenols and their unsaturated constituents in other phenolic species. LITERATURE CITED

Determination of cocaine and benzoylecgonine by direct injection of human urine into a column-switching liquid chromatography system with diode-array detection

“…Several methods have been reported for the identification and quantification of COC and its metabolite in urine samples. These include immunoassay [1][2][3], gas chromatography [4][5][6][7], gas chromatography-mass spectrometry (GC-MS) [2,[8][9][10][11][12][13][14][15][16][17], and time-of-flight secondary ion mass spectrometry (TOF-SIMS) [18]. Currently, immunoassays are frequently employed for the identification of cocaine and its metabolites in biological fluids but it cannot be considered as a reliable quantitative assay because normally all positive results must be confirmed by other quantitative methods.…”

Reliability of the Cloned-Enzyme Donor Immunoassay (CEDIA) for Cocaine in Human Serum in the Range between the Detection Limit and the Cut-Off

“…Gas chromatographic techniques for the analysis of cocaine and its metabolites in urine require derivatization of the polar metabolites and have used flame ionization, electron capture or nitrogen-phosphorus detection (Javaid et al [66], Wallace et al [67], Jain et al [68], Kogan et al [69], von Minden and D'Amato [70]). Gas chromatography / mass spectrometry (GC/MS) is the method of choice for many analysts (Isenschmid et al [71], Hime et al [72], Corburt and Koves [73], de la Torre et al [74], Smirnow and Logan [75], Spanbauer et al [76]).…”

Chromatographic Methodologies for Analysis of Cocaine and Its Metabolites in Biological Matrices

“…On the other hand, isolation of amphoteric compounds, including BE, is more complex and requires a careful choice of the appropriate solvent and regulation of the pH. Wallace et al (1976) described a method for cocaine and BE determination in urine samples of patients who undergone surgery with cocaine anaesthesia. After extraction into a chloroform-ethanol solution (80/20%), the organic phase was evaporated to dryness at 55 ºC, under a stream of filtered air.…”