Gas gangrene-associated gliding motility is regulated by the Clostridium perfringens CpAL/VirSR system

Valeriani, Renzo G.; Beard, LaMonta L.; Moller, Abraham G; Ohtani, Kaori; Vidal, Jorge E.

doi:10.1016/j.anaerobe.2020.102287

Cited by 5 publications

(2 citation statements)

References 64 publications

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“…Additionally, the presence of other plasmid-borne resistance genes fexA , erm (Q), aac(6′)-aph(2 ″), erm (B), and lnu (P), particularly erm (A) and fexA that are located on a segment flanked by IS element IS Vlu1 or IS 1216E together with optrA , may contribute to the co-selection of optrA. C. perfringens is one of the main causes of gas gangrene and foodborne diseases in humans ( 45 ), which can transfer between food animals and humans through animal products. AMR of C. perfringens from food animals can pose serious threats to food safety and public health.…”

Section: Discussionmentioning

confidence: 99%

Genome characteristics of the optrA -positive Clostridium perfringens strain QHY-2 carrying a novel plasmid type

Wu,

Li,

Fang

et al. 2023

mSystems

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Clostridium perfringens is a bacterial species of importance to both public and animal health. The gene optrA is the first gene that confers resistance to the tedizolid, a last-resort antimicrobial agent in human medicine. Herein, we whole-genome sequenced and analyzed one optrA -positive C. perfringens strain QHY-2 from Tibetan sheep in Qinghai province and identified one optrA plasmid pQHY-2. The plasmid shared similar structure with the optrA -positive plasmids p2C45 and p21-D-5b previously identified in C. perfringens , demonstrating the potential horizontal transmission of the optrA plasmids among C. perfringens strains. Annotation of the optrA -positive plasmids showed optrA and erm (A) located on a segment flanked by IS element IS 1216E , and fexA , optrA, and erm (A) located on a segment flanked by IS element IS Vlu1 , which revealed the possible dissemination mechanism. Additionally, a Tn 6218 -like transposon carrying aac(6′)-aph(2″ ) and erm (B) was also detected on pQHY-2, demonstrating the transposition of Tn 6218 and spread of antibiotic resistance among Clostridium bacteria. Molecular analysis indicated the optrA -positive plasmids belonged to a plasmid type distinct from the pCW3-like plasmids, pCP13-like plasmids, or pIP404-like plasmids. Further structure analysis showed they might be formed by inserting segments into plasmid pCPCPI53k-r1_1, which coexist with two pCW3-like plasmids and one pCP13-like plasmid in C. perfringens strain CPI 53k-r1 isolated from a healthy human in Finland. IMPORTANCE Antimicrobial resistance is now a global concern posing threats to food safety and public health. The pCW3-like plasmids can encode several main toxin genes and three antibiotic resistance genes (ARGs), including tetA (P), tetB (P), and erm (B), which used to be considered as the main carrier of ARGs in Clostridium perfringens . In this study, we found the optrA plasmids, which belonged to a novel plasmid type, could also harbor many other ARGs, indicating this type of plasmid might be the potential repository of ARGs in C. perfringens . Additionally, this type of plasmid could coexist with the pCW3-like plasmids and pCP13-like plasmids that encoded toxin genes associated with gastrointestinal diseases, which showed the potential threat to public health.

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Section: Discussionmentioning

confidence: 99%

Genome characteristics of the optrA -positive Clostridium perfringens strain QHY-2 carrying a novel plasmid type

Wu,

Li,

Fang

et al. 2023

mSystems

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“…The pathogenicity is mainly mediated by secreting various extracellular toxins and enzymes, such as B-like toxin (NetB), CPA, perfringolysin O (PFO), collagenase (ColA) and sialidases, which play irreplaceable roles in the cytotoxicity to host cells and the development of NE in chickens ( Mehdizadeh Gohari et al., 2021 ; Fathima and Hakeem, 2022 ). In addition, virulence-associated traits such as adhesion, gliding motility and biofilm formation should also be conducive to the survival and integrated virulence mechanism of C. perfringens ( Valeriani et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens

Liu,

Wu,

Zhao

et al. 2023

Front. Cell. Infect. Microbiol.

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Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi. The present study investigated the effects of baicalin-aluminum on the virulence-associated traits and virulence genes expression of C. perfringens CVCC2030, it also evaluated the in vivo therapeutic effect on NE. The results showed that baicalin-aluminum inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity to Caco-2 cells, downregulated the expression of genes encoding for clostridial toxins and extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), and sialidases (NanI, NanJ). Additionally, baicalin-aluminum was found to negatively regulate the expression of genes involved in quorum sensing (QS) communication, including genes of Agr QS system (agrB, agrD) and genes of VirS/R two-component regulatory system (virS, virR). In vivo experiments, baicalin-aluminum lightened the intestinal lesions and histological damage, it inhibited pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) expression in the jejunal and ileal tissues. Besides, baicalin-aluminum alleviated the upregulation of C. perfringens and Escherichia coli and raised the relative abundance of Lactobacillus in the ileal digesta. This study suggests that baicalin-aluminum may be a potential candidate against C. perfringens infection by inhibiting the virulence-associated traits and virulence genes expression.

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Effect of allyl isothiocyanate on the growth and virulence of Clostridium perfringens and its application on cooked pork

Lin

Addo

et al. 2023

Food Research International

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Gas gangrene-associated gliding motility is regulated by the Clostridium perfringens CpAL/VirSR system

Cited by 5 publications

References 64 publications

Genome characteristics of the optrA -positive Clostridium perfringens strain QHY-2 carrying a novel plasmid type

Genome characteristics of the optrA -positive Clostridium perfringens strain QHY-2 carrying a novel plasmid type

Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens

Effect of allyl isothiocyanate on the growth and virulence of Clostridium perfringens and its application on cooked pork

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