Gas Diffusion Electrodes for Use in an Amperometric Enzyme Biosensor

Hämmerle, Martin; Achmann, Sabine; Moos, Ralf

doi:10.1002/elan.200804321

Cited by 21 publications

(17 citation statements)

References 25 publications

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“…It is clearly seen that electron transfer rate did not increase at higher NQS concentrations since 1 mmol L −1 NQS was enough to accelerate the reaction sufficiently. This result is similar to that with a previously reported NQS mediated biosensor …”

Section: Resultssupporting

confidence: 93%

“…Bareket et al obtained a sensitivity value of 600 nA mmol L −1 using a screen printed electrode modified with carbon nanotubes . Other reported sensitivity values were 2 nA mmol L −1 , approximately 1000 nA mmol L −1 , 5000 nA mmol L −1 . The enhanced sensor sensitivity we obtained gives the possibility of the creation of fast electron transfer between enzyme and electrode via the mediator NQS.…”

Section: Resultsmentioning

confidence: 50%

“…Sensitivity was calculated from the slope of the calibration curve and found to be 15 000 nA mmol L −1 (500 nA ppm −1 ). Even the amount of immobilized enzyme (240 µg) was lower than most of formaldehyde biosensor studies, higher sensitivity was observed for the prepared electrode in comparison with the other papers , , , , , , , , . In those papers, immobilized enzymes amounts were at mg level and electrode surface areas were extremely large.…”

Section: Resultsmentioning

confidence: 80%

“…The enzyme FDH catalyzes the oxidation of formaldehyde to formic acid, while NAD + is reduced to NADH. The mediator NQS is reduced to NQSH 2 while NAD + is recycled , . Finally, the mediator is electrochemically re‐oxidized to NQS on − 0.23 V vs. Ag/AgCl on the working electrode, and the measured current is related to the formaldehyde concentration of the rain water samples.…”

Section: Resultsmentioning

confidence: 99%

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Electrochemical biosensor for detection of formaldehyde in rain water

Ozoner

Erhan

Yılmaz

et al. 2012

J of Chemical Tech & Biotech

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BACKGROUD: This study describes the construction of an electrochemical formaldehyde biosensor based on poly(glycidyl methacrylate‐co‐3‐methylthienyl methacrylate)/formaldehyde dehydrogenase/polypyrrole [poly(GMA‐co‐MTM)/FDH/PPy] composite film electrode. Formaldehyde dehydrogenase (FDH) was chemically immobilized via the epoxy groups of the glycidyl methacrylate (GMA) side chain of the polymer. Formaldehyde measurements were conducted in 0.1 mol L−1, pH 8 phosphate buffer solution (PBS) including 0.1 mol L−1 KCl, 0.5 mmol L−1 of NAD+ (cofactor of the enzyme) and 1 mmol L−1 of 1,2‐napthoquinone‐4‐sulfonic acid sodium salt (NQS) as mediator with an applied potential of − 0.23 V (vs. Ag/AgCl, 3 mol L−1 NaCl). Analytical parameters of the biosensor were calculated and discussed. The biosensor was tested in rain water samples. RESULTS: Sensitivity was found to be 15 000 per mmol L−1 (500 nA ppm−1) in a linear range between 0.1 ppm and 3 ppm (3.3–100 µmol L−1). A minimum detectable concentration of 4.5 ppb (0.15 µmol L−1) (S/N = 3) with a relative standard deviation (RSD) of 0.73% (n = 5) was obtained from the biosensor. Response time of the biosensor was very short, reaching 99% of its maximum response in about 4 s. The biosensor was also tested for formaldehyde measurements in rain water samples. Formaldehyde concentrations in samples were calculated using the proposed biosensor with recovery values ranged between 92.2 and 97.7% in comparison with the colorimetric Nash method. CONCLUSION: The poly(GMA‐co‐MTM)/FDH/PPy) electrode showed excellent measurement sensitivity in comparison with other formaldehyde biosensor studies. Strong chemical bonding between the enzyme and the copolymer was created via the epoxy groups of the composite film. The proposed biosensor could be used successfully in rain waters without a pretreatment step. © 2012 Society of Chemical Industry

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical biosensor for detection of formaldehyde in rain water

Ozoner

Erhan

Yılmaz

et al. 2012

J of Chemical Tech & Biotech

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“…Most work currently intends to develop amperometric enzyme biosensors that detect a gaseous analyte. 6 However, biosensors that could detect a specific antibody or antigen in blood are a much-needed application with respect to energy generator integration.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor Using a Gas Diffusion Layer as an Immobilization Matrix

Kim¹,

Oh²,

Kim³

et al. 2011

Bulletin of the Korean Chemical Society

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The modification of a gas diffusion layer (GDL), a vital component in polymer electrolyte fuel cells, is described here for use in the electrochemical detection of antibody-antigen biosensors. Compared to other substrates (gold foil and graphite), mouse anti-rHBsAg monoclonal antibody immobilized on gold-coated GDL (G-GDL) detected analytes of goat anti-mouse IgG antibody-ALP using a relatively low potential (−0.0021 V vs. Ag/AgCl 3 M NaCl), indicating that undesired by-reactions during electrochemical sensing should be avoided with G-GDL. The dependency of the signal against the concentration of analytes was observed, demonstrating the possibility of quantitative electrochemical biosensors based on G-GDL substrates. When a sandwich method was employed, target antigens of rHBsAg with a concentration as low as 500 ng/mL were clearly measured. The detection limit of rHBsAg was significantly improved to 10 ng/mL when higher concentrations of the 4-aminophenylphosphate monosodium salt (APP) acting on substrates were used for generating a redox-active product. Additionally, it was shown that a BSA blocking layer was essential in improving the detection limit in the G-GDL biosensor.

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Application of gas diffusion electrodes in bioelectrochemical syntheses and energy conversion

Horst

Mangold

Holtmann

2015

Biotech & Bioengineering

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Combining the advantages of biological components (e.g., reaction specificity, self-replication) and electrochemical techniques in bioelectrochemical systems offers the opportunity to develop novel efficient and sustainable processes for the production of a number of valuable products. The choice of electrode material has a great impact on the performance of bioelectrochemical systems. In addition to the redox process at the electrodes, interactions of biocatalysts with electrodes (e.g., enzyme denaturation or biofouling) need to be considered. In recent years, gas diffusion electrodes (GDEs) have proved to be very attractive electrodes for bioelectrochemical purposes. GDEs are porous electrodes, that posses a large three-phase boundary surface. At this interface, a solid catalyst supports the electrochemical reaction between gaseous and liquid phase. This mini-review discusses the application of GDEs in microbial and enzymatic fuel cells, for microbial electrolysis, in biosensors and for electroenzymatic synthesis reactions.

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Gas Diffusion Electrodes for Use in an Amperometric Enzyme Biosensor

Cited by 21 publications

References 25 publications

Electrochemical biosensor for detection of formaldehyde in rain water

Electrochemical biosensor for detection of formaldehyde in rain water

Electrochemical Immunosensor Using a Gas Diffusion Layer as an Immobilization Matrix

Application of gas diffusion electrodes in bioelectrochemical syntheses and energy conversion

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