Gas Cluster Ion Beams for Secondary Ion Mass Spectrometry

Abstract

Gas cluster ion beams (GCIBs) provide new opportunities for bioimaging and molecular depth profiling with secondary ion mass spectrometry (SIMS). These beams, consisting of clusters containing thousands of particles, initiate desorption of target molecules with high yield and minimal fragmentation. This review emphasizes the unique opportunities for implementing these sources, especially for bioimaging applications. Theoretical aspects of the cluster ion/solid interaction are developed to maximize conditions for successful mass spectrometry. In addition, the history of how GCIBs have become practical laboratory tools is reviewed. Special emphasis is placed on the versatility of these sources, as size, kinetic energy, and chemical composition can be varied easily to maximize lateral resolution, hopefully to less than 1 micron, and to maximize ionization efficiency. Recent examples of bioimaging applications are also presented.

Applications of mass spectrometry imaging and top-down proteomic techniques for clinical and biomedical research

“…sphingomyelin) and is found in white matter regions. 10 Mouse brain lipids have been well characterized through MSI and our results were in general agreement with lipids previously identified. [11][12][13] For this data, absorption mode processing (AMP) was applied for a faster acquisition in the FTICR; this allows the samples to be acquired at a lower resolution (512k) but processed at a higher resolution (1 M).…”

“…9 Validation, with 100+ samples (all handled the same way), multi-center studies, and testing in multiple organisms are some of the ways to confirm a biomarker. 10 The infamous 2002 publication in the Lancet Petricoin et al left an major impact upon the field of mass spectrometry (MS), 11 and as a result statisticians and computer programmers have made significant contributions to the identification of disease biomarkers. 12,13…”

“…8,9 Due to their unique strengths and weaknesses, SIMS, IMC, and MALDI are considered complementary techniques. 10,11 IMC is the only current technique that can analyze the proteome of single cells to sufficient depth-as many as 36 proteins simultaneously-to identify cell-types in situ. 12 The sensitivity and dynamic range of IMC is enhanced by signal amplification (multiple reporter ions per antibody), reporter ions (rare earth metals and mass filtering to limit background), and the selectivity of reporter-labeled antibodies.…”

“…9 Matrix assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) data provides the mass and spatial distribution molecules, particularly for the analysis of tissue. 10 MSI has proven to be a valuable tool for clinical diagnostics along with expertise from a trained pathologist to distinguish between healthy and diseased tissue. 11 However, there are some challenges to MSI that limit its widespread use.…”

Воздействие Наноразмерных Анионов Кремний-Молибденовой Кислоты На Плазматическую Мембрану Фибробластов Эмбриона Человека

“…Такая информация доступна современным вариантам метода времяпролетной масс-спектрометрии вторичных ионов (ToF-SIMS). В последнее десятилетие этот аналитический метод стал часто применяться для химического картирования биологических структур [24][25][26][27][28][29][30][31][32]. Однако для решения задач, связанных с изучением природы элементарных актов начального воздействия анионов ПОМ на плазматические мембраны, метод ToF-SIMS до настоящей работы не применялся.…”