Gas chromatography profile of estrogens: Application to pregnancy urine

Adessi, G. L.; Eichenberger, D.; Nhuan, Tran Quang; Jayle, M F

doi:10.1016/0039-128x(75)90031-8

Cited by 32 publications

(2 citation statements)

References 7 publications

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“…Following pH adjustment the oestrogens are re-extracted into an organic solvent which must be washed free of base prior to further processing. In the more usual case, acidic contaminants are removed from steroid extracts by washing with a base, thereby leaving the steroids in the organic phase (Adessi et al, 1975;Adlercreutz and Jarvenpaa, 1982;Ballerini et al, 1980;Barron et al, 1979;Numazawa et al, 1985).…”

Section: Cleanup and Fractionationmentioning

confidence: 97%

“…Hence, when total steroid concentrations (i.e. conjugated plus unconjugated steroid) are required (Adessi et al, 1982;Unnerus, 1979), the hydrolysis of ether and ester linkages is an obligatory step. Although considerable variation exists between procedures (Barron et al, 1979), it is usual to hydrolyze the steroid conjugates before extraction (Purdy et al, 1980).…”

Section: Hydrolysismentioning

confidence: 99%

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Chromatography as a reference technique for the determination of clinically important steroids

Robards

Towers

1990

Biomedical Chromatography

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Chromatographic methods (paper chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry) for the determination of clinically important steroids in biological specimens are reviewed. The emphasis is on the use of gas chromatography, gas chromatography-mass spectrometry and high performance liquid chromatography as reference rather than routine techniques. Chromatographic methods are compared with colorimetric, fluorimetric and radioimmunoassay procedures in terms of simplicity of operation, cost and ability to analyse large numbers of specimens. The importance of correct specimen collection and storage are discussed. Sample preparation techniques for the various analytical methods are described. These include extraction of free and conjugated steroids from serum, plasma, urine and saliva by solvent partition, with polymer-based resins such as Amberlite XAD-2, DEAE-Sephadex and Sephadex resins bonded with various other function groups and, more recently, with chemically bonded reversed-phase silicas.

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Section: Cleanup and Fractionationmentioning

confidence: 97%

Section: Hydrolysismentioning

confidence: 99%