Gas chromatography-mass spectrometry of lipopolysaccharide 3-hydroxy fatty acids: comparison of pentafluorobenzoyl and trimethylsilyl methyl ester derivatives

Mielniczuk, Zbigniew; Alugupalli, Srinivas; Mielniczuk, Eugenia; Larsson, Lennart

doi:10.1016/0021-9673(92)85305-d

Cited by 22 publications

(17 citation statements)

References 10 publications

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“…The compounds were identified by their fragmentation patterns and by com par iso n with authentic samples (The Wiley, 1989). Hydroxylated compounds present in the crude ex tracts were either methylated with diazomethane or silylated with bis-(trimethylsilyl)-acetamide (Mielniczuk, 1992), followed by GC/MS. For methylation, 10 [il of a solution of diazomethane in pentane were added to 10 |il of the crude extract, concentrated (Klimetzek et al, 1989), and subm it ted to GC/MS analysis.…”

Section: Chemical Analysesmentioning

confidence: 99%

The Exocrinology of the Queen Bumble Bee Bombus terrestris (Hymenoptera: Apidae, Bombini)

Hefetz

Taghizadehr

Francke

1996

Zeitschrift Für Naturforschung C

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Bumble Bee, Queen, Glands, Volatile Secretions, Identification Chemical analyses are presented of prominent exocrine glands of queen B om bus terrestris including mandibular, labial and hypopharyngeal glands in the head, Dufour's gland and tarsal glands. A plethora o f about 500 substances were identified belonging to various ali phatic compounds including hydrocarbons, various classes of esters, alcohols, methyl ketones and fatty acids. A group of chiral hydroxy acids and their butanoic acid esters are reported for the first time in bees. 0 939-5075/96/0500-0409 $ 06.00

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Section: Chemical Analysesmentioning

confidence: 99%

The Exocrinology of the Queen Bumble Bee Bombus terrestris (Hymenoptera: Apidae, Bombini)

Hefetz

Taghizadehr

Francke

1996

Zeitschrift Für Naturforschung C

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“…More attention should be paid on the quantitative data evaluation possibilities offered by the GC-MS. Several efforts have been made that , involve fragmentation in qualitative terms, and this has been applied mostly to aliphatic carboxylic acids with few exceptions [2,3,24,27,31,[45][46][47]51]. Acids have been investigated, both in their free form [4][5][6][7][8][9][10] and as their methyl [11][12][13][14], pentafluorobenzoyl [14,15], TMS [16][17][18][19][20][21][22][23], and tert.-butyldimethylsilyl (TBDMS) [24][25][26][27] esters. Mainly quadrupole type mass detectors, in few cases also ITD [1-3, 5-7, 10, 26, 51] have been used.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

1998

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SummaryFragmentation patterns and quantitation possibilities of gas chromatography-mass spectrometry (GC-MS) with Ion Trap Detection (ITD) are reported for the trimethylsilyl (TMS) derivatives of selected aliphatic and aromatic/cyclohexanoic, mono-, di-and polyhydroxy/ methoxy carboxylic acids, o-phosphoric acid, proline and 5-hydroxymethylfurfurol (HMF) -(common in natural matrices, such as fruits, honey etc.). In order to maintain stability of derivatives, their stock solutions were diluted with hexamethyldisilazane. Quantitation was carried out simultaneously on the basis both of the total ion current (TIC) and selective fragment ion (SFI) values. Data obtained proved that (i) the fragmentation of different TMS acids provided very informative, utilizable characteristics, that were also suitable for quantitation; (

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“…The presence of 3-hydroxy fatty acids (derived from LPS) in organic dust has been measured by high performance liquid chromatography (Morris et al, 1988) and GC-MS (Sonesson et al, 1988;Sonesson et a1.,1990). Although liquid chromatography (LC) and gas chromatography (GC) both provide excellent separations, conventional LC and GC detectors do not provide the exquisite selectivity of mass spectrometry often necessary for trace analysis of complex samples such as dust (Mielniczuk et al, 1992;Mielniczuk et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Quantification of Muramic Acid, a Marker for Bacterial Peptidoglycan, in Dust Collected from Hospital and Home Air-Conditioning Filters using Gas Chromatography - Mass Spectrometry

Fox

Rosario

1994

Indoor Air

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The development of standardized non‐culture‐based approaches capable of assessing microbial contarnination of airborne dust is sorely needed. Direct chemical analysis has previously been successfully used for measuring components unique to Gram‐negative bacteria. In the present study, dust from primary filters of hospital air‐conditioning intake systems (which filter incoming outdoor and recirculated air) and dust from secondary room filters (filtering primarily indoor air) were analyzed. Dust from home air‐condition‐ing filters (which also filter outdoor air, with recirculation) were also analyzed. Muramic acid is an aminosugar present in Gram‐positive and Gram‐negative bacterial cell walls and can serve as a measure of bacterial contamination in dust. Samples were analyzed by gas chromatography‐mass spectrometry after hydrolysis and conversion of released sugars (including muramic acid) to alditol acetates. Primary hospital filters contained 26.3 ± 10.0 ng of muramic acid/mg dust while secondary filters contained 5.3 ± 5.4 ng/mg. The level of inuramic acid in home air‐conditioner dust was 31.7 ± 13.4 ng/mg. This study of dust collected from air‐conditioners demonstrates the feasibility of chemical assessment of the microbial contamination of indoor air.

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Gas chromatography-mass spectrometry of lipopolysaccharide 3-hydroxy fatty acids: comparison of pentafluorobenzoyl and trimethylsilyl methyl ester derivatives

Cited by 22 publications

References 10 publications

The Exocrinology of the Queen Bumble Bee Bombus terrestris (Hymenoptera: Apidae, Bombini)

The Exocrinology of the Queen Bumble Bee Bombus terrestris (Hymenoptera: Apidae, Bombini)

Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

Quantification of Muramic Acid, a Marker for Bacterial Peptidoglycan, in Dust Collected from Hospital and Home Air-Conditioning Filters using Gas Chromatography - Mass Spectrometry

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