Gas Chromatography-Mass Spectrometry (GC-MS) Assay of Bio- Active Compounds and Phytochemical Analyses in Three Species of Apocynaceae

Willie, Peggy; Uyoh, Edak Aniedi; Aikpokpodion, Peter O.

doi:10.5530/pj.2021.13.49

Cited by 11 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, phytol and squalene also showed the various biological activities as reported for Coldenia procumbens Linn [2]. Research work also revealed GC-MS profiling of some other Apocyanaceae family namely Gongronema latifolium, Vincetoxicum rossicum and Marsdenia edulis species revealed biologically functional compounds with therapeutic properties including linoleic acid, phytol, neophytadiene, nhexadecanoic acid, squalene, transfarnesol, 5pentadecen-7-yne, and mercaptoacetic acid [49]. Squalene, another constituent identified in GCMS is a natural triterpene known to decrease immobility time in FST [50].…”

Section: Resultsmentioning

confidence: 66%

GC-MS Analysis of the Rauwolfia vomitoria Ethanol Extracts

Asoro

Ebuehi

Igwo-Ezikpe

2021

EJMP

View full text Add to dashboard Cite

Bioactive compounds are the frontline potent agents in both nutraceuticals and pharmaceutical industries. The bioactive compounds are gaining much importance for their ability in enhancing resistance to various diseases and to improve the health of people both by traditional and modern ways of administrations. R. vomitoria is one of the medicinal plants used traditionally to manage hypertension, diabetes and mental disorder. This present study sought to characterize the bioactive components of R. vomitoria leaf and root ethanol extracts using Gas-Chromatography-Mass Spectrophotometry (GC-MS). The results of the GC-MS analysis provide different peaks indicating the presence of 22 phytochemicals in the plant leaf and 16 phytochemicals in the root. The major bioactive compounds in the leaf were squalene (18.69%), phytol (16.47%), n-hexadecanoic acid (15.68%), 7-tetradecenal, (Z) (12.90%), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z)-, (9.56%) and others, while the roots contains; cis-vaccenic acid (32.13%), n-hexadecanoic acid (15.41%), (E)-9-octadecenoic acid ethyl ester (9.83), cyclohexanecarbonitrile 1-(-4- chlorophenyl (9.45%), 8H-azeceno[5,4-b] indol-8-one, 5-ethylidene (7.66%) and other minor compounds. Pharmacological activities of these compounds indicated that the compounds present in the leaf of the plant can be used as a crude drug which could be developed into a novel drug. Some of these compounds have antimicrobial, antioxidant, hepatoprotective, hypocholesterolemic as well as cancer preventive activities amongst others. The findings suggest that there is an indication that both R. vomitoria leaves and roots contain potent bioactive compounds that may be linked to its beneficial effects on health, with the leaf taking the lead. It is therefore recommended as a plant of phytopharmaceutical significance.

show abstract

Section: Resultsmentioning

confidence: 66%

GC-MS Analysis of the Rauwolfia vomitoria Ethanol Extracts

Asoro

Ebuehi

Igwo-Ezikpe

2021

EJMP

View full text Add to dashboard Cite

show abstract

“…On the other hand, the production of 1-chlorononadecane can be due to the degradation of stable chlorine compounds and the reactivity of chlorine with carbon compounds in the mill over time. 52 It is noteworthy that impact from the balls could create compounds and re-reactions due to the splitting of C-C bonds, so lower molecular mass compounds are produced. 53,54 corresponding to chlorine compounds (peaks below 750 cm À1 ) were significantly reduced and almost disappeared.…”

Section: Influence Of MC Parameters On De-cl Of Petrochemical Wastewa...mentioning

confidence: 99%

“…However, there is a very weak peak at approximately 751 cm À1 which, according to GC/MS analysis results, could be due to the presence of 1-chlorononadecane in the purified sample. 10,30,48,52 A new absorption band that appeared at 860 cm À1 could be referred to =C-H bonds or intermediate compounds such as benzocycloheptatriene.…”

Section: Influence Of MC Parameters On De-cl Of Petrochemical Wastewa...mentioning

confidence: 99%

“…The peak around 900 cm À1 could be linked to the =C-H bending vibration and the peak around 2000 cm À1 could be linked to the carbon triple bond. 48,52,58 It seems there was a defect in the carbon structure of organic pollutants, disrupting their original regular structure. Moreover, according to the intermediate products and the results of FTIR and GC/MS, it can be concluded that the formation of carbonaceous materials was due to side reactions that occurred after the degradation of stable organic pollutants.…”

Section: Influence Of MC Parameters On De-cl Of Petrochemical Wastewa...mentioning

confidence: 99%

See 1 more Smart Citation

Mechanochemical dechlorination of petrochemical sludge through a planetary ball mill and using industrial wastes as additives

Alavi

Adabi

Sadani

et al. 2022

Env Prog and Sustain Energy

View full text Add to dashboard Cite

Mechanochemical treatment causes the formation of mineral Cl compounds during the degradation of polychlorinated compounds by planetary ball mill. In the present study, the three types of industrial waste comprising of sludge from petrochemical wastewater treatment plants as source of pollutants, scrap iron and petrochemical water treatment plant sludge as co‐milling reagents were used. The raw samples and the milled products were analyzed by gas chromatography–mass spectrometry, Fourier transform infrared spectroscopy, Raman Spectroscopy, and X‐ray diffraction. Further analyses such as ion chromatography and X‐ray fluorescence were performed. The analysis results confirmed that the milling process degraded persistent organic pollutants and showed that major changes occurred in the organic phases, carbon and chlorine bonds. The yield of chloride ions was investigated using Ion Chromatography. The highest percentage of de‐Cl products (95.6%) was achieved after 6 h in equal proportions of reagents. Furthermore, CaCl2 and FeCl2 were present in the final milling product.

show abstract

“…Antioxidants, such as flavonoids, are among them. To date, a number of analytical methods have been developed for the determination of flavonoids, such as HPLC [13][14][15][16][17][18], gas chromatography [19,20], capillary electrophoresis [21][22][23], voltammetry [24][25][26], and spectrophotometry [27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin

Apyari

Furletov

Kalinin

et al. 2022

Sensors

View full text Add to dashboard Cite

Microfluidic paper-based analytical devices (µPADs) represent one of the promising green analytical strategies for low-cost and simple determination of various analytes. The actual task is the development of such devices for quantitation of antioxidants, e.g., flavonoids. In this paper, possibilities of a novel three-reagent µPAD including silver nitrate, 4-nitrophenyldiazonium tetrafluoroborate, and iron(III) chloride as reagents are assessed with respect to the determination of dihydroquercetin. It is shown that all the three reagents produce different colorimetric responses that can be detected by a mini-spectrophotometer–monitor calibrator or by a smartphone. The method is applicable to direct measuring high contents of dihydroquercetin (the linearity range is 0.026–1 mg mL−1, and the limit of detection is 7.7 µg mL−1), which is favorable for many dietary supplements. The analysis of a food supplement was possible with the relative standard deviations of 9–26%, which is satisfactory for quantitative and semiquantitative determinations. It was found that plotting a calibration graph in 3D space of the three reagents’ responses allows us to distinguish dihydroquercetin from its close structural analogue, quercetin.

show abstract

Gas Chromatography-Mass Spectrometry (GC-MS) Assay of Bio- Active Compounds and Phytochemical Analyses in Three Species of Apocynaceae

Cited by 11 publications

References 0 publications

GC-MS Analysis of the Rauwolfia vomitoria Ethanol Extracts

GC-MS Analysis of the Rauwolfia vomitoria Ethanol Extracts

Mechanochemical dechlorination of petrochemical sludge through a planetary ball mill and using industrial wastes as additives

A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin

Contact Info

Product

Resources

About