Gas Chromatography/Mass Spectrometry-Based Metabolite Profiling of Nutrients and Antinutrients in Eight Lens and Lupinus Seeds (Fabaceae)

Abstract

Lens culinaris and several Lupinus species are two legumes regarded as potential protein resources aside from their richness in phytochemicals. Consequently, characterization of their metabolite composition seems warranted to be considered as a sustainable commercial functional food. This study presents a discriminatory holistic approach for metabolite profiling in accessions of four lentil cultivars and four Lupinus species via gas chromatography/mass spectrometry. A total of 107 metabolites were identified, encompassing organic and amino acids, sugars, and sterols, along with antinutrients, viz., alkaloids and sugar phosphates. Among the examined specimens, four nutritionally valuable accessions ought to be prioritized for future breeding to include Lupinus hispanicus, enriched in organic ( ca. 11.7%) and amino acids ( ca. 5%), and Lupinus angustifolius, rich in sucrose ( ca. 40%), along with two dark-colored lentil cultivars 'verte du Puy' and 'Black Beluga' enriched in peptides. Antinutrient chemicals were observed in Lupinus polyphyllus, owing to its high alkaloid content. Several species-specific markers were also revealed using multivariate data analyses.

Effect of grilling as processing method on Zea mays (corn) metabolites composition as analyzed via SPME GC‐MS and chemometrics

“…Pyruvic acid ( 2 ) a ketocarboxylic acid was found more in grilled corn due to thermal degradation of furan molecules (De Rovira, ). It should be noted that the absence of phytic acid, an antinutrient forming chelates with mineral and protein decreasing their bioavailability, in investigated corn variety suggest its good quality (Farag, Khattab, et al, ).…”

Nutrient and Sensory Metabolites Profiling of Averrhoa Carambola L. (Starfruit) in the Context of Its Origin and Ripening Stage by GC/MS and Chemometric Analysis

“…Analysis of primary metabolites followed the exact protocol detailed in our previous work [24,[70][71][72]. 100 µL of 50% aqueous extract (prepared by extracting 100 mg of fruit skin and pulp in 5 mL 50% MeOH) was evaporated under nitrogen till dryness.…”

Importance of Lupinus albescens in agricultural and food-related areas: A review

“…The bioactive compounds are formed by secondary plant metabolism and may cause different pathologies (Wink 1992). In a recent study, Farag et al (2018) identified 107 metabolites from different species of Lupinus, comprising organic compounds, sugars, and sterols. However, different approaches must be carried out to explore other factors related to the composition such as collection site, seasonal variation, and extraction conditions.…”