Gas Chromatography - Mass Spectrometry Analysis and In vitro Antioxidant Activity of the Ethanolic Extract of the Leaves of Tabernaemontana divaricata

Abstract

Objective: To identify phytoconstituents present in an ethanolic extract of the leaves of Tabernaemontana divaricata and to evaluate its in-vitro antioxidant potential. Methods: The extract was subjected to gas chromatography-mass spectrometry analysis to identify phytoconstituents, and screened for hydroxyl, superoxide and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and metal-chelating activity as a measure of potential antioxidant activity. Results: GC-MS analysis of the extract revealed the presence of 96 phytoconstituents, of which 17 are reported to be bioactive and 11 of these to possess antioxidant potential. When tested in-vitro, the extract exhibited the most potent radical-scavenging activity at a maximum concentration of 10 mg/ml, scavenging effects of 64%, 67% and 69% and corresponding half maximal inhibitory concentration (IC 50 ) values of 6.7 mg/ml, 6.8 mg/ml and 6.2 mg/ml on hydroxyl, superoxide and DPPH radicals, respectively. Ascorbic acid used as a standard (10 mg/ml) showed scavenging effects of 73%, 73% and 75% and corresponding IC 50 values of 5.3 mg/ml, 5.8 mg/ml and 5.2 mg/ml, respectively, on hydroxyl, superoxide and DPPH radicals. At 10 mg/ml, the extract and an ethylenediaminetetraacetic acid standard exhibited 68% and 78%, respectively, chelation of ferrous ions; at the same concentration, the reducing power of the extract and that of a butylated hydroxytoluene standard was found to be 3.855 and 4.308, respectively. Conclusion: These observations strongly suggest that the ethanolic extract of T. divaricata leaves has potent in-vitro antioxidant activity and thereby could act as a possible therapeutic agent for oxidative stressinduced pathological states.

Ethnopharmacology, phytochemistry, and biotechnological advances of family Apocynaceae: A review

“…Using the same methods, the antioxidative potential of Wrightia tinctoria was also evaluated and the ethanolic flower extract showed maximum scavenging potential against DPPH radicals with IC 50 of 43.16 μg/ml (Ramalakshmi et al, 2012). Another member of the family, Tabernaemontana divaricata, was also studied for its antioxidant activity using metal chelating, DPPH, and superoxide radical scavenging methods (Anbukkarasi, Thomas, Sundararajan, & Geraldine, 2016). Qualitative and quantitative DPPH scavenging assays were studied to determine the antioxidative potential of Tabernaemontana divaricata leaves extract (Rumzhum, Rahman, & Kazal, 2012).…”

Review on the Pharmacognostical, Phytochemical and Pharmacological aspects of Tabernaemontana coronaria

“…The extract exhibited the most potent radical scavenging activity at a maximum concentration of 10 mg/ml. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the extract reveals the presence 96 phytoconstituents, 17 are reported bioactive and 11 possess antioxidant potential [64] .…”

Characterization of the complete chloroplast genome of Tabernaemontana divaricate (Apocynaceae), a valuable and endangered plant

“…2012 ; Anbukkarasi et al. 2016 ). In recent years, researches on T. divaricate have mainly focused on pharmacological properties (Naidoo et al.…”