Gas chromatography–mass spectrometric analysis of bonded long chain fatty acids in a single zebrafish egg by ultrasound-assisted one-step transmethylation and extraction

Assessment of the fatty acid composition of different parts of zebrafish fed diets incorporated with linseed and sunflower oils

“…A total of 21 fatty acids were identified. Among it, the most abundant fatty acids were: 16:0 (saturated fatty acid; SFA), 18:1n-9 (monounsaturated fatty acid; MUFA), 18:2n-6, and 18:3n-3 (polyunsaturated fatty acid; PUFA), coherently with results published by other researchers (Li et al, 2009;Monroig et al, 2012). However, comparing the results, some fatty acids were significantly altered, proving the efficiency of the bioactive incorporation, according to the different compositions of the feeds.…”

Chemical and genetic probes for analysis of protein palmitoylation

“…1(B)) [71,72]. The iFAT approach integrates extraction, transmethylation and stable isotope labeling into a single step with the aid of ultrasound sonication.…”

Effects of a high fat diet on long-chain fatty acids composition in rats serum and liver

“…Although a variety of methods, including flow injection titrimetric (Saad, Ling, & Jab, 2007), colorimetric (Guianvarc'h, Drujon, Leang, & Courtois, 2006), enzymatic (Bychkov & Shekhovtsova, 2003) and X-ray spectrometry (Sitko, Zawisza, Kowalewska, Kocot, & Polowniak, 2011), have been employed to assess the total content of FA in biological material, capillary gas chromatography (Cazzola & Cestaro, in press;Hubbard, Blum, Bickel, & Heller, 2003;Li, Yue, Li, Hu, & Zhong, 2009;Politi, Mari, Furlanetto, Bravo, & Bertol, 2011;Zhao, Liu, Nyman, & Jönsson, 2007) and high performance liquid chromatography (HPLC) (Bayer, Mosandl, & Thaçi, 2005;Bravi, Perretti, & Montanari, 2006;Li et al, 2011) should be the first choice to analyze both the total content and the fatty acyl composition of the FA pool.…”