Gas chromatography applied to the lactulose—mannitol intestinal permeability test

Dumas, Frédéric; Aussel, Christian; Pernet, Pascal; Martin, C; Giboudeau, J

doi:10.1016/0378-4347(94)00041-7

Cited by 23 publications

(18 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative standard deviation (RSD) for D-mannitol and lactulose were 5.48 and 11.14%, respectively. In comparison, the published RSDs for the determination of D-mannitol and lactulose in human urine were 8.9 and 9.3% (Dumas et al, 1994).…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test

Klein¹,

Moravcová²,

Kleinová³

et al. 2007

J. Anim. Feed Sci.

View full text Add to dashboard Cite

The aim of this study was to utilize the lactulose/mannitol test for assessment of intestinal permeability (IP) in preruminant calves. IP was increased experimentally by administration of Indomethacin. Both sugar markers were determined simultaneously as silyl-derivates in 5 h urinary production using the gas liquid chromatography technique. The index of IP was determined as the ratio between urinary recovery (%) of 10 g lactulose to 5 g D-mannitol. The IP index in control calves was 0.242±0.048. Doses of 20 and 60 mg Indomethacin did not significantly affect IP, but the IP index was significantly enhanced in calves receiving 120 mg of drug (0.541±0.091; P<0.01). Results of this study show that ratio of lactulose to D-mannitol in urine reflected the treatment by the highest dose of Indomethacin and that the test may be used for determination of IP in preruminant calves.

show abstract

Section: Discussionmentioning

confidence: 91%

“…The GLC method for the determination of trimethylsilyl ethers of lactulose and D-mannitol was slightly modified from that of Dumas et al (1994). In the our modification, the silylation was carried out at ambient temperature and xylitol was used as internal standard for both analytes.…”

Section: Discussionmentioning

confidence: 99%

Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test

Klein¹,

Moravcová²,

Kleinová³

et al. 2007

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…Results were below the lowest point of the calibration curves defined in our method (25 and 500 mg/l for lactulose and mannitol, respectively) [20]. Hence, the doses of 66 mg lactulose and 50 mg mannitol were chosen in all further experiments as they allowed the same chromatographic conditions as those used in human tests.…”

Section: Choice Of the Dose Of Lactulose And Mannitol And Effect Of Omentioning

confidence: 99%

“…Mannitol and lactulose were separated and quantitated by a capillary gas chromatography method, developed by us for tests in humans, as previously described [20]. Briefly, Tri-Sil TBT [TMS-imidazole, bis TMS-acetamide and trimethyl chlorosilane (3:3:2, v/v/v), Pierce, Rockford, Ill., USA] was used for sugar derivatization.…”

Section: Intestinal Permeability Testmentioning

confidence: 99%

Standardization of the Lactulose-Mannitol Test in Rats: Application to Burn Injury

et al. 1998

Self Cite

View full text Add to dashboard Cite

Intestinal permeability can be assessed by measuring urinary mannitol and lactulose excretion after oral administration. This test may be useful as a tool in experimental studies to explore the effects of specific diets intended to promote the repair of the integrity of the gut barrier. In this study we standardized the lactulose-mannitol test in rats and applied it to a burned-rat model. The conditions were: oral administration of an isotonic mixture of 50 mg of mannitol and 66 mg of lactulose, followed by aseptic collection of urine over 4 h. The increase in the lactulose/mannitol ratio in burned rats was due to higher lactulose excretion. These results corroborate those obtained in burn patients and show that our model is suitable for further experiments on nutritional manipulation.

show abstract

“…Urine lactulose and mannitol concentrations were measured using gas chromatography (Dumas et al 1994). The urine samples were first desalinated with Amberlite MB8 resin (Sigma-Aldrich, St. Louis, MO) and then washed three times with milliQ water.…”

Section: Laboratory Analysismentioning

confidence: 99%

The efficacy of anti-mycotoxin feed additives in preventing the adverse effects of wheat naturally contaminated with Fusarium mycotoxins on performance, intestinal barrier function and nutrient digestibility and retention in weanling pigs

et al. 2015

View full text Add to dashboard Cite

. 2015. The efficacy of anti-mycotoxin feed additives in preventing the adverse effects of wheat naturally contaminated with Fusarium mycotoxins on performance, intestinal barrier function and nutrient digestibility and retention in weanling pigs. Can. J. Anim. Sci. 95: 197Á209. This study was designed to determine the effect of feeding deoxynivalenol (DON) contaminated wheat to growing pigs on growth performance, digestibility and retention of nitrogen (N), phosphorus (P) and calcium (Ca). Secondly, we wished to evaluate the potential of four commercial anti-mycotoxin additives in preventing the adverse effects of DON. Sixty piglets (6.0 kg body weight) were assigned to six different corn-soybean meal-wheat diets (control diet: B0.5 mg kg(1 DON; contaminated diet (DONcontaminated): 4 mg kg (1 ; and four contaminated diets supplemented with a different anti-mycotoxin additive: DON' GLUC (glucomannan), DON'YBP (yeast, live bacteria, enzymes, plant extracts), DON'ALU (aluminosilicate) and DON'PC (preservation components). Piglets were housed individually in pens for 7 d and then transferred to metabolic crates for urine and feces collection for 7 d. At the end of these 2 wk, mannitol and lactulose doses were given by oral administration. Urine was collected for 24 h for an evaluation of barrier integrity of intestinal mucosa. The piglets were then euthanatized and intestinal samples collected for morphology studies. Feeding DON-contaminated wheat reduced average daily feed intake, average daily gain and G:F ratio compared with the control diet (PB0.05). Only DON'PC diet restored the growth performance of piglets fed DON-contaminated diet. Daily retention of N and P was not affected by DON contamination or anti-mycotoxin additives, but retention of Ca was higher in piglets fed the DON-contaminated diets than the control diet. DON-contaminated diet reduced digestibility of dry matter, gross energy and fat, villi height in jejunum, and recovery of mannitol and lactulose compared with the control diet (PB0.05). Finally, DON concentrations in serum from piglets fed the DON-contaminated diets were higher than in the control diet (PB0.05). Piglets fed DONcontaminated wheat had reduced growth possibly caused by impaired jejunal morphology and decreased digestibility of energy and fat.

show abstract

Gas chromatography applied to the lactulose—mannitol intestinal permeability test

Cited by 23 publications

References 13 publications

Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test

Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test

Standardization of the Lactulose-Mannitol Test in Rats: Application to Burn Injury

The efficacy of anti-mycotoxin feed additives in preventing the adverse effects of wheat naturally contaminated with Fusarium mycotoxins on performance, intestinal barrier function and nutrient digestibility and retention in weanling pigs

Contact Info

Product

Resources

About