Gas-chromatography and UV-spectroscopy of Hymenoptera venoms obtained by trivial centrifugation

Fox, Eduardo Gonçalves Paterson; Xu, Meng; Wang, Lei; Chen, Li; Lu, Yongyue

doi:10.1016/j.dib.2018.03.101

Cited by 6 publications

(3 citation statements)

References 2 publications

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“…These insect alkaloids can be extracted efficiently by centrifugation of live ants or by solvent immersion of the live fire ants (23) followed by purification using silica-gel chromatography. Dosing and tracking the extracted solenopsin has been described as likely based on their absorbance peak near 232 nm (24). Crystals of piperine are extracted from the black pepper.…”

Section: Alkaloids Extractionmentioning

confidence: 99%

The Biochemistry, Toxicology, and Uses of the Pharmacologically Active Phytochemicals: Alkaloids, Terpenes, Polyphenols, and Glycosides

Awuchi

2019

J. Food Pharm. Sci

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The research focused on the biochemistry and the medicinal uses of alkaloids, terpenes,polyphenols, and glycosides. Several parts of plants such as bark, root, leaf, stem, etc. are being used torevert abnormalities back to normal, prevent illnesses, or allay symptoms. These plant parts containbioactive compounds such as terpenes, glycosides, polyphenols, and alkaloids. Alkaloids have a rangeof pharmacological activities such as antiasthma (e.g. ephedrine), anticancer (e.g. homoharringtonine),antimalarial (e.g. quinine). Other alkaloids possess stimulant (e.g. cocaine, caffeine, theobromine, nicotine)and psychotropic activities (e.g. psilocin), and have been widely used as recreational drugs orin entheogenic rituals. Alkaloids can be toxic too (e.g. tubocurarine, atropine). Although alkaloids act ondiverse metabolic systems in humans and animals, they almost uniformly induce a bitter taste. Thecharacteristics and number of the structures of phenol in polyphenols underlie the unique chemical,physical, and biological (metabolic, therapeutic, toxic, etc.) properties of specific members of the class;e.g. ellagitannin and tannic acid. Generally, foods often contain complex mixtures of polyphenols. Severalpolyphenolic extracts, such as from grape seeds, olive pulp, maritime pine bark, or grape skin, are sold asingredients for functional foods, cosmetics, and dietary supplements. Terpenoids and terpenes are theprimary components of the essential oils of most types of flowers plants. Essential oils are used widely asscents and fragrances in perfumery and also in traditional medicine, such as aromatherapy. Similarly,many plant glycosides are used as medicines. Amygdalin and laetrile (a synthetic derivative) wereinvestigated as potential drugs for cancer treatment and were promoted as alternative medicine;nevertheless, they are ineffective and dangerous. Pharmacologically active phytochemicals can haveuseful applications, as well as safety concerns.

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Section: Alkaloids Extractionmentioning

confidence: 99%

The Biochemistry, Toxicology, and Uses of the Pharmacologically Active Phytochemicals: Alkaloids, Terpenes, Polyphenols, and Glycosides

Awuchi

2019

J. Food Pharm. Sci

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“…There are currently well defined protocols for separating and analysing fire ant venom alkaloids (Fox et al 2013;Yu et al 2014) as well as a number of published raw chromatograms available as chemical references (e.g. Fox et al 2018a, Fox et al 2018b) allowing for reproducible results.…”

Section: Introductionmentioning

confidence: 99%

Chemical blueprints to identifying fire ants: overview on venom alkaloids

Fox¹

2018

Preprint

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Solenopsis fire ants are remarkably difficult to identify using morphological characters, particularly from the most abundant minor workers. The present manuscript introduces a set of chemical tools to facilitate species diagnosis from field-collected fire ant samples, herein focusing on minor workers.Fire ants from different localities (native and invaded) were analysed using gas-chromatography.Samples were collected from the field into organic solvents; sampling effort included 14 species, and a suspected hybrid. A total of 32 piperidine alkaloids were spotted and tentatively identified and representative relative chemical proportions for minor workers are presented for the first time for a number of species. It is hoped that the provided info will prove useful to researchers working on fire ants in future studies. Further compounds are being analysed for additional auxiliary tools.

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“…cytolytic, hemolytic, allergenic, proin ammatory, insecticidal, antimicrobial, and nontoxic functions such as roles in chemical communication involving trail and sex pheromones, deterrents, and aggregators [5,6,9,10]. Reactions of human beings to the sting of red imported re ants vary from a burning sensation to severe anaphylactic shock and even death [11].…”

mentioning

confidence: 99%

Identification and Diversity of Bacterial Communities Associated with the Venom Glands of the Fire Ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

Yang

Chen

et al. 2020

Preprint

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Background Ant-microbe interactions that naturally occur in the venom microenvironment remain mostly unknown. To best of our knowledge, no research exists that shows the identity and diversity of bacterial communities in the fires ants, and what adaptive advantages that venom-associated microbes might offer to their hosts or that hosts might provide to venom-associated bacteria. This study assessed the diversity and identity of bacteria associated with the venom glands of Solenopsis invicta and compared this community with the bacterial communities of two other stinging ants, Solenopsis geminata and Diacamma rugosum. Results The major phylum associated with the venom glands of S. invicta (workers, alates, and queens) and S. geminata (workers) was Proteobacteria, while Firmicutes were abundant in the venom glands of D. rugosum (workers). Tenericutes were also more abundant in the venom glands of S. invicta queens than in those of workers and alates. The relative abundance of Spiroplasma in S. geminata was significantly higher than those in D. rugosum and S. invicta. A microbial diversity analysis of venom glands of worker ants of Solenopsis species showed that the relative abundances of Bacillus and Lactococcus were higher in Guangxi S. invicta workers than in workers collected from Guangzhou. However, the abundance of Lactococcus in workers of D. rugosum was higher than those in workers of S. geminata from Guangxi and S. invicta collected from Guangzhou. Conclusions This study provides the first insight into the microbiota in the venom glands of S. invicta, S. geminata, and D. rugosum, which might contribute to a more comprehensive view of the role of bacteria in the synthesis or degradation of active venom components in the host. We hypothesized that the differences in bacterial communities of three ant species could be due to the local adaptation of insects along with the coevolution of bacteria and hosts.

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Gas-chromatography and UV-spectroscopy of Hymenoptera venoms obtained by trivial centrifugation

Cited by 6 publications

References 2 publications

The Biochemistry, Toxicology, and Uses of the Pharmacologically Active Phytochemicals: Alkaloids, Terpenes, Polyphenols, and Glycosides

The Biochemistry, Toxicology, and Uses of the Pharmacologically Active Phytochemicals: Alkaloids, Terpenes, Polyphenols, and Glycosides

Chemical blueprints to identifying fire ants: overview on venom alkaloids

Identification and Diversity of Bacterial Communities Associated with the Venom Glands of the Fire Ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

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